Reviewed, UniProtKB/Swiss-Prot Q9PIZ3 (KHSE_CAMJE)

Names and origin

Protein names

Recommended name:
    Homoserine kinase
      Short name=HSK
      Short name=HK
    EC=2.7.1.39

Gene names

Name:	thrB
Ordered Locus Names:	Cj0134

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-homoserine = ADP + O-phospho-L-homoserine. HAMAP MF_00384
Pathway	Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5. HAMAP MF_00384
Subcellular location	Cytoplasm Potential HAMAP MF_00384.
Sequence similarities	Belongs to the GHMP kinase family. Homoserine kinase subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphorylation Inferred from electronic annotation. Source: InterPro threonine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP homoserine kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 292	292	Homoserine kinase HAMAP MF_00384		PRO_0000156558
Regions
	Nucleotide binding	84 – 94	11	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PIZ3-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 187D5770641B99ED	FASTA	292	32,501
	10 20 30 40 50 60 MKILVPATSA NLGPGFDCLG LSLKLFNETQ IQKSGVFSIS IGGEGSDNIF LKKNNIFVNI 70 80 90 100 110 120 FYEIYEKLSG KKDNFRFIFQ NNIPLSRGLG SSSAVIVGAI ASAYYMSGFK VEKECILDEA 130 140 150 160 170 180 LIYENHPDNI APATLGGFVC SLVEKNKVYS IKKEIDKDLA AVVVIPNLAM STEQSRQALA 190 200 210 220 230 240 KNLSFNDAVF NLSHASFLTA CFLEKKYEFL KFASQDKLHE INRMKNLPEL FEVQKFALEN 250 260 270 280 290 KALMSTLSGS GSSFFSLAFK DDALALAKKI QTKFKDFRVQ YLEFDDNGFE IC

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34305.1.
PIR	F81430.
RefSeq	YP_002343594.1.
3D structure databases
SMR	Q9PIZ3. Positions 2-275.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PIZ3. 1 interaction.
Genome annotation databases
GeneID	904490.
GenomeReviews	Gene locus Cj0134 in contig AL111168_GR.
KEGG	cje:Cj0134.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646290.
OMA	NNALMST.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0134-MONOMER.
BRENDA	2.7.1.39. 255835.
Family and domain databases
HAMAP	MF_00384. Homoser_kinase. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR006203. GHMP_knse_ATP_bd_CS. IPR000870. Homoserine_kin. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
Pfam	PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF000676. Homoser_kin. 1 hit.
PRINTS	PR00958. HOMSERKINASE.
TIGRFAMs	TIGR00191. thrB. 1 hit.
PROSITE	PS00627. GHMP_KINASES_ATP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KHSE_CAMJE

Accession

Primary (citable) accession number: Q9PIZ3
Secondary accession number(s): Q0PC03

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)