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Protein

4-hydroxy-tetrahydrodipicolinate reductase

Gene

dapB

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate.UniRule annotation

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.Curated

Catalytic activityi

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H.UniRule annotation

Pathwayi: L-lysine biosynthesis via DAP pathway

This protein is involved in step 4 of the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Aspartokinase (lysC)
  2. Aspartate-semialdehyde dehydrogenase (asd)
  3. 4-hydroxy-tetrahydrodipicolinate synthase (dapA)
  4. 4-hydroxy-tetrahydrodipicolinate reductase (dapB)
This subpathway is part of the pathway L-lysine biosynthesis via DAP pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-tetrahydrodipicolinate from L-aspartate, the pathway L-lysine biosynthesis via DAP pathway and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei131Proton donor/acceptorUniRule annotation1
Binding sitei132SubstrateUniRule annotation1
Active sitei135Proton donorUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 13NAD(P)UniRule annotation6
Nucleotide bindingi75 – 77NAD(P)UniRule annotation3
Nucleotide bindingi99 – 102NAD(P)UniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Diaminopimelate biosynthesis, Lysine biosynthesis
LigandNAD, NADP

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-190-MONOMER
UniPathwayiUPA00034; UER00018

Names & Taxonomyi

Protein namesi
Recommended name:
4-hydroxy-tetrahydrodipicolinate reductaseUniRule annotation (EC:1.17.1.8UniRule annotation)
Short name:
HTPA reductaseUniRule annotation
Gene namesi
Name:dapBUniRule annotation
Ordered Locus Names:Cj0197c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001414221 – 2424-hydroxy-tetrahydrodipicolinate reductaseAdd BLAST242

Proteomic databases

PaxDbiQ9PIT2
PRIDEiQ9PIT2

Interactioni

Protein-protein interaction databases

IntActiQ9PIT2, 53 interactors
STRINGi192222.Cj0197c

Structurei

3D structure databases

ProteinModelPortaliQ9PIT2
SMRiQ9PIT2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni141 – 142Substrate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the DapB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105DUK Bacteria
COG0289 LUCA
HOGENOMiHOG000227153
KOiK00215
OMAiRESFMPG

Family and domain databases

HAMAPiMF_00102 DapB, 1 hit
InterProiView protein in InterPro
IPR022663 DapB_C
IPR000846 DapB_N
IPR022664 DapB_N_CS
IPR023940 DHDPR_bac
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR20836 PTHR20836, 1 hit
PfamiView protein in Pfam
PF05173 DapB_C, 1 hit
PF01113 DapB_N, 1 hit
PIRSFiPIRSF000161 DHPR, 1 hit
SUPFAMiSSF51735 SSF51735, 2 hits
TIGRFAMsiTIGR00036 dapB, 1 hit
PROSITEiView protein in PROSITE
PS01298 DAPB, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PIT2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKIGIYGAK GRMGKQIEEC LKSETQARIS ILYDKGGNLG ELFEKSDVII
60 70 80 90 100
DFSSPSGTHE LLNYARTMPK PLVIGTTGLD EKILHLMQSA SEVMPIFYAT
110 120 130 140 150
NMSLGVAVLN YLASKASQML RNFDIEILEM HHRHKKDAPS GTAMTLAQSV
160 170 180 190 200
AKARNLELEK VRVSGRDGII GERSKDEIAV MSLRGGDIVG RHTVGFYEDG
210 220 230 240
EFLELNHTAT SRATFAKGAI KIAIWLSKQE AKMYSINDFL GI
Length:242
Mass (Da):26,696
Last modified:October 1, 2000 - v1
Checksum:i6DC65A7925B5EF8F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL34366.1
PIRiD81438
RefSeqiWP_002851754.1, NC_002163.1
YP_002343655.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL34366; CAL34366; Cj0197c
GeneIDi904539
KEGGicje:Cj0197c
PATRICifig|192222.6.peg.194

Similar proteinsi

Entry informationi

Entry nameiDAPB_CAMJE
AccessioniPrimary (citable) accession number: Q9PIT2
Secondary accession number(s): Q0PBU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 110 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health