Q9PIS0 (ARGC_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetyl-gamma-glutamyl-phosphate reductase
Short name=AGPR
EC=1.2.1.38

Alternative name(s):
N-acetyl-glutamate semialdehyde dehydrogenase
Short name=NAGSA dehydrogenase

Gene names

Name:	argC
Ordered Locus Names:	Cj0224

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	N-acetyl-L-glutamate 5-semialdehyde + NADP+ + phosphate = N-acetyl-5-glutamyl phosphate + NADPH. HAMAP MF_00150
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4. HAMAP MF_00150
Subcellular location	Cytoplasm Probable HAMAP MF_00150.
Sequence similarities	Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyl-gamma-glutamyl-phosphate reductase activity Inferred from electronic annotation. Source: EC NAD binding Inferred from electronic annotation. Source: InterPro protein dimerization activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
flaG	Q0PAW9	1	EBI-1195272,EBI-1191365

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	N-acetyl-gamma-glutamyl-phosphate reductase HAMAP MF_00150		PRO_0000112393
Sites
	Active site	147	1	By similarity
Experimental info
	Sequence conflict	76	1	K → E in AAF21802. Ref.1
	Sequence conflict	119	1	E → D in AAF21802. Ref.1
	Sequence conflict	168	1	S → N in AAF21802. Ref.1
	Sequence conflict	194	1	V → I in AAF21802. Ref.1
	Sequence conflict	202	1	N → G in AAF21802. Ref.1
	Sequence conflict	205	1	L → S in AAF21802. Ref.1
	Sequence conflict	296	1	V → I in AAF21802. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9PIS0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D5CE90039C63179C	FASTA	342	38,896
	10 20 30 40 50 60 MKIKVGILGA SGYAGNELVR ILLNHPKVEI SYLGSSSSVG QNYQDLYPNT PLNLCFENKN 70 80 90 100 110 120 LDELELDLLF LATPHKFSAK LLNENLLKKM KIIDLSADFR LKNPKDYELW YKFTHPNQEL 130 140 150 160 170 180 LQNAVYGLCE LYKEEIKKAS LVANPGCYTT CSILSLYPLF KEKIIDFSSV IIDAKSGVSG 190 200 210 220 230 240 AGRSAKVENL FCEVNENIKA YNLALHRHTP EIEEHLSYAA KEKITLQFTP HLVPMQRGIL 250 260 270 280 290 300 ISAYANLKED LQEQDIRDIY TKYYQNNKFI RLLPPQSLPQ TRWVKSSNFA DINFSVDQRT 310 320 330 340 KRVIVLGAID NLIKGAAGQA VQNMNLMFDF DEDEGLKFFA NL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arginine biosynthesis in Campylobacter jejuni TGH9011: determination of the argCOBD cluster." Hani E.K., Ng D., Chan V.-L. Can. J. Microbiol. 45:959-969(1999) [PubMed: 10588044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093219 Genomic DNA. Translation: AAF21802.1. AL111168 Genomic DNA. Translation: CAL34379.1.
PIR	H81439.
RefSeq	YP_002343667.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PIS0.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PIS0. 35 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	906012.
GenomeReviews	Gene locus Cj0224 in contig AL111168_GR.
KEGG	cje:Cj0224.
PATRIC	20057369. VBICamJej33762_0218.
Phylogenomic databases
HOGENOM	HBG294213.
OMA	VCRIAVH.
ProtClustDB	PRK00436.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0224-MONOMER.
Family and domain databases
HAMAP	MF_00150. ArgC_type1. [Tree]
InterPro	IPR023013. AGPR_AS. IPR000706. AGPR_type-1. IPR016040. NAD(P)-bd_dom. IPR000534. Semialdehyde_DH_NAD-bd. IPR012280. Semialdhyde_DH_dimer_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K00145.
Pfam	PF01118. Semialdhyde_dh. 1 hit. PF02774. Semialdhyde_dhC. 1 hit. [Graphical view]
SMART	SM00859. Semialdhyde_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01850. ArgC. 1 hit.
PROSITE	PS01224. ARGC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGC_CAMJE

Accession

Primary (citable) accession number: Q9PIS0
Secondary accession number(s): Q0PBT0, Q9RH00

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program