Q9PIR8 (ARGB_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylglutamate kinase
EC=2.7.2.8

Alternative name(s):
N-acetyl-L-glutamate 5-phosphotransferase
NAG kinase
Short name=AGK

Gene names

Name:	argB
Ordered Locus Names:	Cj0226

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate. HAMAP MF_00082_B
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4. HAMAP MF_00082_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00082_B.
Sequence similarities	Belongs to the acetylglutamate kinase family.
Sequence caution	The sequence AAF21804.1 differs from that shown. Reason: Erroneous initiation. The sequence CAL34381.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetylglutamate kinase activity Inferred from electronic annotation. Source: EC glutamate 5-kinase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Acetylglutamate kinase HAMAP MF_00082_B		PRO_0000112602
Regions
	Region	64 – 65	2	Substrate binding By similarity
Sites
	Binding site	86	1	Substrate By similarity
	Binding site	177	1	Substrate By similarity
	Site	29	1	Transition state stabilizer By similarity
	Site	238	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	62	1	H → R in AAF21804. Ref.1
	Sequence conflict	73 – 76	4	EKLG → ARLF in AAF21804. Ref.1
	Sequence conflict	83	1	N → D in AAF21804. Ref.1
	Sequence conflict	93	1	I → T in AAF21804. Ref.1
	Sequence conflict	172	1	N → D in AAF21804. Ref.1
	Sequence conflict	210	1	N → S in AAF21804. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9PIR8 [UniParc]. Last modified August 16, 2005. Version 2. Checksum: 06770EBBC3E3C96D	FASTA	279	30,519
	10 20 30 40 50 60 MQKYLEKANV LIEALPYIRK FNSKIILIKY GGSAMENEEL KHCVMQDIAL LKLVGLKPII 70 80 90 100 110 120 VHGGGKDISA MCEKLGVKSE FKNGLRVSDK ATIEVASMVL NHINKNLVHS LQNLGVKAIG 130 140 150 160 170 180 LCGKDGALLE CVKKDENLAF VGTIQKVNSK ILEELLEKDF LPIIAPIGMD ENFNTYNINA 190 200 210 220 230 240 DDAACAIAKA LRAEKLAFLT DTAGLYEDFN DKNSLISKIS LEQAKILAPK IEGGMHVKLK 250 260 270 SCIDACENGV KKVHILDGRV KHSLLLEFFT DEGIGTLVG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Arginine biosynthesis in Campylobacter jejuni TGH9011: determination of the argCOBD cluster." Hani E.K., Ng D., Chan V.-L. Can. J. Microbiol. 45:959-969(1999) [PubMed: 10588044] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 43431 / TGH 9011 / Serotype O:3.
[2]	"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF093219 Genomic DNA. Translation: AAF21804.1. Different initiation. AL111168 Genomic DNA. Translation: CAL34381.1. Different initiation.
PIR	B81440.
RefSeq	YP_002343669.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PIR8.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PIR8. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904554.
GenomeReviews	Gene locus Cj0226 in contig AL111168_GR.
KEGG	cje:Cj0226.
PATRIC	20057373. VBICamJej33762_0220.
Phylogenomic databases
HOGENOM	HBG497643.
OMA	GGNAMID.
ProtClustDB	PRK00942.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0226-MONOMER.
Family and domain databases
HAMAP	MF_00082_B. ArgB_B. [Tree]
InterPro	IPR004662. AcgluKinase. IPR001048. Asp/Glu/Uridylate_kinase. IPR001057. Glu/AcGlu_kinase. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit.
KO	K00930.
Pfam	PF00696. AA_kinase. 1 hit. [Graphical view]
PIRSF	PIRSF000728. NAGK. 1 hit.
PRINTS	PR00474. GLU5KINASE.
SUPFAM	SSF53633. Aa_kinase. 1 hit.
TIGRFAMs	TIGR00761. ArgB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGB_CAMJE

Accession

Primary (citable) accession number: Q9PIR8
Secondary accession number(s): Q0PBS8, Q9RGZ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	August 16, 2005
Last modified:	January 25, 2012
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program