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Protein

Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase

Gene

lpxA

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell.UniRule annotation

Catalytic activityi

(R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = [acyl-carrier-protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine.UniRule annotation

Pathwayi: lipid IV(A) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase (lpxA)
  2. UDP-3-O-acyl-N-acetylglucosamine deacetylase (lpxC)
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Lipid-A-disaccharide synthase (lpxB)
  6. Tetraacyldisaccharide 4'-kinase (lpxK)
This subpathway is part of the pathway lipid IV(A) biosynthesis, which is itself part of Glycolipid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine, the pathway lipid IV(A) biosynthesis and in Glycolipid biosynthesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Lipid A biosynthesis, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-246-MONOMER.
UniPathwayiUPA00359; UER00477.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseUniRule annotation (EC:2.3.1.129UniRule annotation)
Short name:
UDP-N-acetylglucosamine acyltransferaseUniRule annotation
Gene namesi
Name:lpxAUniRule annotation
Ordered Locus Names:Cj0274
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001880381 – 263Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferaseAdd BLAST263

Proteomic databases

PaxDbiQ9PIM1.

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIM1. 29 interactors.
STRINGi192222.Cj0274.

Structurei

Secondary structure

1263
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi63 – 66Combined sources4
Beta strandi80 – 83Combined sources4
Beta strandi94 – 96Combined sources3
Turni100 – 103Combined sources4
Beta strandi104 – 108Combined sources5
Beta strandi182 – 185Combined sources4
Beta strandi190 – 194Combined sources5
Helixi196 – 202Combined sources7
Helixi205 – 219Combined sources15
Beta strandi220 – 222Combined sources3
Helixi224 – 232Combined sources9
Helixi238 – 249Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R0SX-ray2.30A1-263[»]
ProteinModelPortaliQ9PIM1.
SMRiQ9PIM1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the transferase hexapeptide repeat family. LpxA subfamily.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105DAF. Bacteria.
COG1043. LUCA.
HOGENOMiHOG000294326.
KOiK00677.
OMAiMAYCHLG.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 3 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PIM1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIHPSAVI EEGAQLGDDV VIEAYAYVSK DAKIGNNVVI KQGARILSDT
60 70 80 90 100
TIGDHSRVFS YAIVGDIPQD ISYKEEQKSG VVIGKNATIR EFATINSGTA
110 120 130 140 150
KGDGFTRIGD NAFIMAYCHI AHDCLLGNNI ILANNATLAG HVELGDFTVV
160 170 180 190 200
GGLTPIHQFV KVGEGCMIAG ASALSQDIVP FCLAEGNRAS IRSLNLVGIR
210 220 230 240 250
RRFDKDEVDR LSRAFKTLFR QGDLKENAKN LLENQESENV KKMCHFILET
260
KRGIPVYRGK NNA
Length:263
Mass (Da):28,651
Last modified:October 1, 2000 - v1
Checksum:iC3D17D1BE12F9F01
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34428.1.
PIRiA81446.
RefSeqiWP_002851756.1. NC_002163.1.
YP_002343716.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34428; CAL34428; Cj0274.
GeneIDi904599.
KEGGicje:Cj0274.
PATRICi20057469. VBICamJej33762_0268.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34428.1.
PIRiA81446.
RefSeqiWP_002851756.1. NC_002163.1.
YP_002343716.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3R0SX-ray2.30A1-263[»]
ProteinModelPortaliQ9PIM1.
SMRiQ9PIM1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PIM1. 29 interactors.
STRINGi192222.Cj0274.

Proteomic databases

PaxDbiQ9PIM1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34428; CAL34428; Cj0274.
GeneIDi904599.
KEGGicje:Cj0274.
PATRICi20057469. VBICamJej33762_0268.

Phylogenomic databases

eggNOGiENOG4105DAF. Bacteria.
COG1043. LUCA.
HOGENOMiHOG000294326.
KOiK00677.
OMAiMAYCHLG.

Enzyme and pathway databases

UniPathwayiUPA00359; UER00477.
BioCyciCJEJ192222:GJTS-246-MONOMER.

Family and domain databases

CDDicd03351. LbH_UDP-GlcNAc_AT. 1 hit.
Gene3Di1.20.1180.10. 1 hit.
HAMAPiMF_00387. LpxA. 1 hit.
InterProiIPR029098. Acetyltransf_C.
IPR001451. Hexapep.
IPR018357. Hexapep_transf_CS.
IPR010137. Lipid_A_LpxA.
IPR011004. Trimer_LpxA-like.
[Graphical view]
PfamiPF13720. Acetyltransf_11. 1 hit.
PF00132. Hexapep. 3 hits.
[Graphical view]
PIRSFiPIRSF000456. UDP-GlcNAc_acltr. 1 hit.
SUPFAMiSSF51161. SSF51161. 1 hit.
TIGRFAMsiTIGR01852. lipid_A_lpxA. 1 hit.
PROSITEiPS00101. HEXAPEP_TRANSFERASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLPXA_CAMJE
AccessioniPrimary (citable) accession number: Q9PIM1
Secondary accession number(s): Q0PBN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.