Reviewed, UniProtKB/Swiss-Prot Q9PIK8 (LPXB_CAMJE)

Names and origin

Protein names

Recommended name:
    Lipid-A-disaccharide synthase
    EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	Cj0288c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity.
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP MF_00392
Pathway	Glycolipid biosynthesis; lipid (IV)A biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-D-glucosamine: step 5/6. HAMAP MF_00392
Sequence similarities	Belongs to the lpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Lipid-A-disaccharide synthase HAMAP MF_00392		PRO_0000190156

Sequences

	Sequence		Length	Mass (Da)
	Q9PIK8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 53DA0DA1979A2C5F	FASTA	364	41,581
	10 20 30 40 50 60 MKTFLVCALE PSANLHLKEV LKAYKKDFGE FELHGIYDES LCKEFDLNSK PLYSSHEFSA 70 80 90 100 110 120 MGFIEVLPLI FKAKKAIKEL ANLSFTQKIN GILCIDSPAF NIPFAKALKK AGSKIPRIYY 130 140 150 160 170 180 ILPQVWAWKK GRIPIIESHF DILASILPFD NQFFNKSTYI GHPLLDEIKE FKNQEDINHT 190 200 210 220 230 240 FSKKDDEKTI AFLPGSRRSE IRRLMPIFKE LSQKFKGKKI LCVPSFNLEK LEVYGDISEF 250 260 270 280 290 300 KIESNTPKVL KKADFAFICS GTATLEAALV GTPFVLAYKA KAIDIFIAKL FVKLKHIGLA 310 320 330 340 350 360 NIFCDFAGKE ALNPEFLQDK VNVLNLYEAY NKYDYKAFFA KVDFLKEYLQ FGSAKNLAKI LNEI

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34441.1.
PIR	F81447.
RefSeq	YP_002343729.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Genome annotation databases
GeneID	904612.
GenomeReviews	Gene locus Cj0288c in contig AL111168_GR.
KEGG	cje:Cj0288c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PIK8. FMPGSRK.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0288C-MON.
BRENDA	2.4.1.182. 255835.
Family and domain databases
HAMAP	MF_00392. [Tree]
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_CAMJE

Accession

Primary (citable) accession number: Q9PIK8
Secondary accession number(s): Q0PBL8

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)