Reviewed, UniProtKB/Swiss-Prot Q9PIG7 (NDK_CAMJE)

Names and origin

Protein names

Recommended name:
    Nucleoside diphosphate kinase
      Short name=NDK
      Short name=NDP kinase
    EC=2.7.4.6
Alternative name(s):
    Nucleoside-2-P kinase

Gene names

Name:	ndk
Ordered Locus Names:	Cj0332c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	137 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. HAMAP MF_00451
Catalytic activity	ATP + nucleoside diphosphate = ADP + nucleoside triphosphate. HAMAP MF_00451
Cofactor	Magnesium By similarity.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the NDK family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	CTP biosynthetic process Inferred from electronic annotation. Source: HAMAP GTP biosynthetic process Inferred from electronic annotation. Source: HAMAP UTP biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside diphosphate kinase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliM	Q0PC73	1	EBI-1194881,EBI-1191095

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 137	137	Nucleoside diphosphate kinase HAMAP MF_00451		PRO_0000136960
Sites
	Active site	115	1	Pros-phosphohistidine intermediate By similarity
	Binding site	9	1	ATP By similarity
	Binding site	57	1	ATP By similarity
	Binding site	85	1	ATP By similarity
	Binding site	91	1	ATP By similarity
	Binding site	102	1	ATP By similarity
	Binding site	112	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PIG7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 53EDA6F707B45C32	FASTA	137	15,142
	10 20 30 40 50 60 MEKTLSIIKP DAVKKGVIGK ILDRFESNGL RIAAMKKVQL SKEQAENFYA VHKERPFFKD 70 80 90 100 110 120 LVEFMISGPV VVSILEGEGA VLKNRDLMGA TNPKEAKAGT IRADFAESID ANAVHGSDSL 130 ENAKIEIEFF FKPNEIC

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34483.1.
PIR	H81452.
RefSeq	YP_002343770.1.
3D structure databases
HSSP	HSSP built from PDB template 1NHK based on UniProtKB P15266.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PIG7. 47 interactions.
Genome annotation databases
GeneID	904656.
GenomeReviews	Gene locus Cj0332c in contig AL111168_GR.
KEGG	cje:Cj0332c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PIG7. NLTGAIT.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0332C-MON.
BRENDA	2.7.4.6. 255835.
Family and domain databases
HAMAP	MF_00451. [Tree]
InterPro	IPR001564. Nuc_diP_kinase_core. [Graphical view]
Gene3D	G3DSA:3.30.70.141. NDK. 1 hit.
PANTHER	PTHR11349. Nuc_diP_kinase_core. 1 hit.
Pfam	PF00334. NDK. 1 hit. [Graphical view]
PRINTS	PR01243. NUCDPKINASE.
ProDom	PD001018. NDK. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00562. NDK. 1 hit. [Graphical view]
PROSITE	PS00469. NDP_KINASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NDK_CAMJE

Accession

Primary (citable) accession number: Q9PIG7
Secondary accession number(s): Q0PBH7

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 26, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)