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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotation

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD (trpGD))
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei43Proton acceptorUniRule annotation1
Active sitei54Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-318-MONOMER.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:Cj0349
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000987621 – 249Tryptophan synthase alpha chainAdd BLAST249

Proteomic databases

PaxDbiQ9PIF1.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIF1. 2 interactors.
STRINGi192222.Cj0349.

Structurei

Secondary structure

1249
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 6Combined sources3
Beta strandi9 – 18Combined sources10
Helixi24 – 32Combined sources9
Helixi33 – 36Combined sources4
Beta strandi40 – 45Combined sources6
Helixi57 – 67Combined sources11
Helixi72 – 81Combined sources10
Beta strandi85 – 91Combined sources7
Helixi94 – 100Combined sources7
Helixi102 – 111Combined sources10
Beta strandi114 – 118Combined sources5
Helixi124 – 126Combined sources3
Helixi128 – 136Combined sources9
Beta strandi143 – 146Combined sources4
Helixi151 – 158Combined sources8
Beta strandi165 – 168Combined sources4
Beta strandi173 – 175Combined sources3
Helixi178 – 192Combined sources15
Beta strandi199 – 204Combined sources6
Helixi208 – 214Combined sources7
Turni215 – 217Combined sources3
Beta strandi218 – 223Combined sources6
Helixi225 – 230Combined sources6
Helixi236 – 246Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3THAX-ray2.37A/B1-249[»]
ProteinModelPortaliQ9PIF1.
SMRiQ9PIF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223816.
KOiK01695.
OMAiPVFICPP.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PIF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDFRKFYKE NANVAYTVLG YPNLQTSEAF LQRLDQSPID ILELGVAYSD
60 70 80 90 100
PIADGEIIAD AAKIALDQGV DIHSVFELLA RIKTKKALVF MVYYNLIFSY
110 120 130 140 150
GLEKFVKKAK SLGICALIVP ELSFEESDDL IKECERYNIA LITLVSVTTP
160 170 180 190 200
KERVKKLVKH AKGFIYLLAS IGITGTKSVE EAILQDKVKE IRSFTNLPIF
210 220 230 240
VGFGIQNNQD VKRMRKVADG VIVGTSIVKC FKQGNLDIIM KDIEEIFKK
Length:249
Mass (Da):27,967
Last modified:October 1, 2000 - v1
Checksum:i79FF358CD7CAC987
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34500.1.
PIRiD81377.
RefSeqiWP_002854645.1. NC_002163.1.
YP_002343787.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34500; CAL34500; Cj0349.
GeneIDi904673.
KEGGicje:Cj0349.
PATRICi20057615. VBICamJej33762_0341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34500.1.
PIRiD81377.
RefSeqiWP_002854645.1. NC_002163.1.
YP_002343787.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3THAX-ray2.37A/B1-249[»]
ProteinModelPortaliQ9PIF1.
SMRiQ9PIF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PIF1. 2 interactors.
STRINGi192222.Cj0349.

Proteomic databases

PaxDbiQ9PIF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34500; CAL34500; Cj0349.
GeneIDi904673.
KEGGicje:Cj0349.
PATRICi20057615. VBICamJej33762_0341.

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223816.
KOiK01695.
OMAiPVFICPP.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciCJEJ192222:GJTS-318-MONOMER.

Family and domain databases

CDDicd04724. Tryptophan_synthase_alpha. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_CAMJE
AccessioniPrimary (citable) accession number: Q9PIF1
Secondary accession number(s): Q0PBG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.