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Protein

Tryptophan synthase alpha chain

Gene

trpA

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate.UniRule annotation

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.UniRule annotation
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Anthranilate phosphoribosyltransferase (trpD (trpGD))
  3. N-(5'-phosphoribosyl)anthranilate isomerase (trpF)
  4. Indole-3-glycerol phosphate synthase (trpC)
  5. Tryptophan synthase beta chain (trpB), Tryptophan synthase alpha chain (trpA)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei43 – 431Proton acceptorUniRule annotation
Active sitei54 – 541Proton acceptorUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-326-MONOMER.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase alpha chainUniRule annotation (EC:4.2.1.20UniRule annotation)
Gene namesi
Name:trpAUniRule annotation
Ordered Locus Names:Cj0349
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 249249Tryptophan synthase alpha chainPRO_0000098762Add
BLAST

Proteomic databases

PaxDbiQ9PIF1.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIF1. 2 interactions.
STRINGi192222.Cj0349.

Structurei

Secondary structure

1
249
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 63Combined sources
Beta strandi9 – 1810Combined sources
Helixi24 – 329Combined sources
Helixi33 – 364Combined sources
Beta strandi40 – 456Combined sources
Helixi57 – 6711Combined sources
Helixi72 – 8110Combined sources
Beta strandi85 – 917Combined sources
Helixi94 – 1007Combined sources
Helixi102 – 11110Combined sources
Beta strandi114 – 1185Combined sources
Helixi124 – 1263Combined sources
Helixi128 – 1369Combined sources
Beta strandi143 – 1464Combined sources
Helixi151 – 1588Combined sources
Beta strandi165 – 1684Combined sources
Beta strandi173 – 1753Combined sources
Helixi178 – 19215Combined sources
Beta strandi199 – 2046Combined sources
Helixi208 – 2147Combined sources
Turni215 – 2173Combined sources
Beta strandi218 – 2236Combined sources
Helixi225 – 2306Combined sources
Helixi236 – 24611Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3THAX-ray2.37A/B1-249[»]
ProteinModelPortaliQ9PIF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpA family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223816.
KOiK01695.
OMAiPVFICPP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PIF1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVDFRKFYKE NANVAYTVLG YPNLQTSEAF LQRLDQSPID ILELGVAYSD
60 70 80 90 100
PIADGEIIAD AAKIALDQGV DIHSVFELLA RIKTKKALVF MVYYNLIFSY
110 120 130 140 150
GLEKFVKKAK SLGICALIVP ELSFEESDDL IKECERYNIA LITLVSVTTP
160 170 180 190 200
KERVKKLVKH AKGFIYLLAS IGITGTKSVE EAILQDKVKE IRSFTNLPIF
210 220 230 240
VGFGIQNNQD VKRMRKVADG VIVGTSIVKC FKQGNLDIIM KDIEEIFKK
Length:249
Mass (Da):27,967
Last modified:October 1, 2000 - v1
Checksum:i79FF358CD7CAC987
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34500.1.
PIRiD81377.
RefSeqiWP_002854645.1. NC_002163.1.
YP_002343787.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34500; CAL34500; Cj0349.
GeneIDi904673.
KEGGicje:Cj0349.
PATRICi20057615. VBICamJej33762_0341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34500.1.
PIRiD81377.
RefSeqiWP_002854645.1. NC_002163.1.
YP_002343787.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3THAX-ray2.37A/B1-249[»]
ProteinModelPortaliQ9PIF1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PIF1. 2 interactions.
STRINGi192222.Cj0349.

Proteomic databases

PaxDbiQ9PIF1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34500; CAL34500; Cj0349.
GeneIDi904673.
KEGGicje:Cj0349.
PATRICi20057615. VBICamJej33762_0341.

Phylogenomic databases

eggNOGiENOG4105F6H. Bacteria.
COG0159. LUCA.
HOGENOMiHOG000223816.
KOiK01695.
OMAiPVFICPP.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciCJEJ192222:GJTS-326-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRPA_CAMJE
AccessioniPrimary (citable) accession number: Q9PIF1
Secondary accession number(s): Q0PBG0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.