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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the decarboxylation of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP).UniRule annotation

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.UniRule annotation

Pathwayi: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei8SubstrateUniRule annotation1
Binding sitei30SubstrateUniRule annotation1
Active sitei60Proton donorUniRule annotation1
Binding sitei117SubstrateUniRule annotation1
Binding sitei177SubstrateUniRule annotation1
Binding sitei186SubstrateUniRule annotation1
Binding sitei206Substrate; via amide nitrogenUniRule annotation1
Binding sitei207SubstrateUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-349-MONOMER.
UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylaseUniRule annotation (EC:4.1.1.23UniRule annotation)
Alternative name(s):
OMP decarboxylaseUniRule annotation
Short name:
OMPDCaseUniRule annotation
Short name:
OMPdecaseUniRule annotation
Gene namesi
Name:pyrFUniRule annotation
Ordered Locus Names:Cj0381c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001345351 – 279Orotidine 5'-phosphate decarboxylaseAdd BLAST279

Proteomic databases

PaxDbiQ9PIC1.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIC1. 23 interactors.
STRINGi192222.Cj0381c.

Structurei

Secondary structure

1279
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 6Combined sources5
Helixi12 – 21Combined sources10
Turni22 – 24Combined sources3
Beta strandi28 – 31Combined sources4
Helixi33 – 39Combined sources7
Helixi41 – 50Combined sources10
Beta strandi54 – 61Combined sources8
Helixi65 – 76Combined sources12
Turni77 – 79Combined sources3
Beta strandi81 – 86Combined sources6
Helixi87 – 89Combined sources3
Helixi91 – 101Combined sources11
Beta strandi104 – 106Combined sources3
Beta strandi109 – 113Combined sources5
Helixi121 – 128Combined sources8
Helixi132 – 145Combined sources14
Beta strandi149 – 152Combined sources4
Turni155 – 157Combined sources3
Helixi158 – 164Combined sources7
Beta strandi169 – 173Combined sources5
Helixi192 – 197Combined sources6
Beta strandi201 – 205Combined sources5
Helixi207 – 210Combined sources4
Helixi215 – 226Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RU6X-ray1.80A/B/C/D1-279[»]
ProteinModelPortaliQ9PIC1.
SMRiQ9PIC1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 67Substrate bindingUniRule annotation10

Sequence similaritiesi

Belongs to the OMP decarboxylase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4106EG9. Bacteria.
COG0284. LUCA.
HOGENOMiHOG000226071.
KOiK01591.
OMAiNFKIFLD.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01200_B. OMPdecase_type1_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PIC1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLCVALDLS TKEECLQLAK ELKNLDIWLK VGLRAYLRDG FKFIEELKKV
60 70 80 90 100
DDFKIFLDLK FHDIPNTMAD ACEEVSKLGV DMINIHASAG KIAIQEVMTR
110 120 130 140 150
LSKFSKRPLV LAVSALTSFD EENFFSIYRQ KIEEAVINFS KISYENGLDG
160 170 180 190 200
MVCSVFESKK IKEHTSSNFL TLTPGIRPFG ETNDDQKRVA NLAMARENLS
210 220 230 240 250
DYIVVGRPIY KNENPRAVCE KILNKIHRKN ISENDIEQNY EVIQQKEWDM
260 270
CNHFEEWIKT RPDKEHALKE FYAKCGIKY
Length:279
Mass (Da):32,331
Last modified:October 1, 2000 - v1
Checksum:iC395A4569A7338A8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34531.1.
PIRiC81381.
RefSeqiWP_002858700.1. NC_002163.1.
YP_002343818.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34531; CAL34531; Cj0381c.
GeneIDi904704.
KEGGicje:Cj0381c.
PATRICi20057677. VBICamJej33762_0372.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34531.1.
PIRiC81381.
RefSeqiWP_002858700.1. NC_002163.1.
YP_002343818.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3RU6X-ray1.80A/B/C/D1-279[»]
ProteinModelPortaliQ9PIC1.
SMRiQ9PIC1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PIC1. 23 interactors.
STRINGi192222.Cj0381c.

Proteomic databases

PaxDbiQ9PIC1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34531; CAL34531; Cj0381c.
GeneIDi904704.
KEGGicje:Cj0381c.
PATRICi20057677. VBICamJej33762_0372.

Phylogenomic databases

eggNOGiENOG4106EG9. Bacteria.
COG0284. LUCA.
HOGENOMiHOG000226071.
KOiK01591.
OMAiNFKIFLD.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.
BioCyciCJEJ192222:GJTS-349-MONOMER.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01200_B. OMPdecase_type1_B. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR014732. OMPdecase.
IPR018089. OMPdecase_AS.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR01740. pyrF. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPYRF_CAMJE
AccessioniPrimary (citable) accession number: Q9PIC1
Secondary accession number(s): Q0PBC9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.