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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei131L-tryptophanUniRule annotation1
Binding sitei182ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 10ATPUniRule annotation3
Nucleotide bindingi16 – 17ATPUniRule annotation2
Nucleotide bindingi143 – 145ATPUniRule annotation3
Nucleotide bindingi189 – 193ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-361-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:Cj0388
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366131 – 319Tryptophan--tRNA ligaseAdd BLAST319

Proteomic databases

PaxDbiQ9PIB4
PRIDEiQ9PIB4

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIB4, 3 interactors
STRINGi192222.Cj0388

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi15 – 20Combined sources6
Helixi22 – 27Combined sources6
Turni28 – 31Combined sources4
Beta strandi35 – 38Combined sources4
Helixi40 – 43Combined sources4
Turni44 – 46Combined sources3
Helixi50 – 66Combined sources17
Turni71 – 73Combined sources3
Beta strandi74 – 78Combined sources5
Helixi79 – 81Combined sources3
Helixi84 – 92Combined sources9
Helixi93 – 95Combined sources3
Helixi98 – 102Combined sources5
Helixi105 – 112Combined sources8
Helixi119 – 134Combined sources16
Beta strandi138 – 141Combined sources4
Helixi144 – 146Combined sources3
Helixi147 – 164Combined sources18
Beta strandi172 – 174Combined sources3
Beta strandi187 – 189Combined sources3
Helixi192 – 194Combined sources3
Beta strandi199 – 201Combined sources3
Helixi204 – 212Combined sources9
Helixi227 – 229Combined sources3
Helixi231 – 236Combined sources6
Helixi237 – 239Combined sources3
Helixi242 – 254Combined sources13
Helixi259 – 273Combined sources15
Helixi275 – 286Combined sources12
Helixi288 – 317Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M5WX-ray2.32A/B1-319[»]
3TZLX-ray2.15A/B1-319[»]
ProteinModelPortaliQ9PIB4
SMRiQ9PIB4
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PIB4

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 17"HIGH" regionUniRule annotation9
Motifi189 – 193"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059939
KOiK01867
OMAiQRMTQFK

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PIB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLTGLQPS GDLHIGNYFG AIKQMVDAQE KSQMFMFIAN YHAMTSSQDG
60 70 80 90 100
EKLKQNSLKA AAAFLSLGID PQKSVFWLQS DVKEVMELYW ILSQFTPMGL
110 120 130 140 150
LERAHSYKDK VAKGLSASHG LFSYPVLMAA DILLFDTRIV PVGKDQIQHV
160 170 180 190 200
EIARDIALKV NNEWGEIFTL PEARVNEEVA VVVGTDGAKM SKSYQNTIDI
210 220 230 240 250
FSSEKTLKKQ ISSIVTDSTA LEDPKDHENC NIFKIAKLFL DESGQKELQI
260 270 280 290 300
RYEKGGEGYG HFKIYLNELV NAYFKEAREK YNELLEKPSH LKEILDFGAT
310
KARKIAQEKM QKIYEKIGL
Length:319
Mass (Da):36,084
Last modified:October 1, 2000 - v1
Checksum:iCB620B2B67AA6666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL34538.1
PIRiB81382
RefSeqiWP_002858719.1, NC_002163.1
YP_002343825.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL34538; CAL34538; Cj0388
GeneIDi904711
KEGGicje:Cj0388
PATRICifig|192222.6.peg.379

Similar proteinsi

Entry informationi

Entry nameiSYW_CAMJE
AccessioniPrimary (citable) accession number: Q9PIB4
Secondary accession number(s): Q0PBC2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

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