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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-356-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:Cj0388
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366131 – 319Tryptophan--tRNA ligaseAdd BLAST319

Proteomic databases

PaxDbiQ9PIB4.

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIB4. 3 interactors.
STRINGi192222.Cj0388.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 7Combined sources5
Helixi15 – 20Combined sources6
Helixi22 – 27Combined sources6
Turni28 – 31Combined sources4
Beta strandi35 – 38Combined sources4
Helixi40 – 43Combined sources4
Turni44 – 46Combined sources3
Helixi50 – 66Combined sources17
Turni71 – 73Combined sources3
Beta strandi74 – 78Combined sources5
Helixi79 – 81Combined sources3
Helixi84 – 92Combined sources9
Helixi93 – 95Combined sources3
Helixi98 – 102Combined sources5
Helixi105 – 112Combined sources8
Helixi119 – 134Combined sources16
Beta strandi138 – 141Combined sources4
Helixi144 – 146Combined sources3
Helixi147 – 164Combined sources18
Beta strandi172 – 174Combined sources3
Beta strandi187 – 189Combined sources3
Helixi192 – 194Combined sources3
Beta strandi199 – 201Combined sources3
Helixi204 – 212Combined sources9
Helixi227 – 229Combined sources3
Helixi231 – 236Combined sources6
Helixi237 – 239Combined sources3
Helixi242 – 254Combined sources13
Helixi259 – 273Combined sources15
Helixi275 – 286Combined sources12
Helixi288 – 317Combined sources30

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M5WX-ray2.32A/B1-319[»]
3TZLX-ray2.15A/B1-319[»]
ProteinModelPortaliQ9PIB4.
SMRiQ9PIB4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PIB4.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi9 – 17"HIGH" region9
Motifi189 – 193"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PIB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLTGLQPS GDLHIGNYFG AIKQMVDAQE KSQMFMFIAN YHAMTSSQDG
60 70 80 90 100
EKLKQNSLKA AAAFLSLGID PQKSVFWLQS DVKEVMELYW ILSQFTPMGL
110 120 130 140 150
LERAHSYKDK VAKGLSASHG LFSYPVLMAA DILLFDTRIV PVGKDQIQHV
160 170 180 190 200
EIARDIALKV NNEWGEIFTL PEARVNEEVA VVVGTDGAKM SKSYQNTIDI
210 220 230 240 250
FSSEKTLKKQ ISSIVTDSTA LEDPKDHENC NIFKIAKLFL DESGQKELQI
260 270 280 290 300
RYEKGGEGYG HFKIYLNELV NAYFKEAREK YNELLEKPSH LKEILDFGAT
310
KARKIAQEKM QKIYEKIGL
Length:319
Mass (Da):36,084
Last modified:October 1, 2000 - v1
Checksum:iCB620B2B67AA6666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34538.1.
PIRiB81382.
RefSeqiWP_002858719.1. NC_002163.1.
YP_002343825.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34538; CAL34538; Cj0388.
GeneIDi904711.
KEGGicje:Cj0388.
PATRICi20057691. VBICamJej33762_0379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34538.1.
PIRiB81382.
RefSeqiWP_002858719.1. NC_002163.1.
YP_002343825.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3M5WX-ray2.32A/B1-319[»]
3TZLX-ray2.15A/B1-319[»]
ProteinModelPortaliQ9PIB4.
SMRiQ9PIB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PIB4. 3 interactors.
STRINGi192222.Cj0388.

Proteomic databases

PaxDbiQ9PIB4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34538; CAL34538; Cj0388.
GeneIDi904711.
KEGGicje:Cj0388.
PATRICi20057691. VBICamJej33762_0379.

Phylogenomic databases

eggNOGiENOG4105C31. Bacteria.
COG0180. LUCA.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-356-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9PIB4.

Family and domain databases

CDDicd00806. TrpRS_core. 1 hit.
Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B. 1 hit.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYW_CAMJE
AccessioniPrimary (citable) accession number: Q9PIB4
Secondary accession number(s): Q0PBC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.