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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei192 – 1921ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. tryptophan-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. tryptophanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-364-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:Cj0388
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 319319Tryptophan--tRNA ligasePRO_0000136613Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PIB4. 3 interactions.
STRINGi192222.Cj0388.

Structurei

Secondary structure

1
319
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 75Combined sources
Helixi15 – 206Combined sources
Helixi22 – 276Combined sources
Turni28 – 314Combined sources
Beta strandi35 – 384Combined sources
Helixi40 – 434Combined sources
Turni44 – 463Combined sources
Helixi50 – 6617Combined sources
Turni71 – 733Combined sources
Beta strandi74 – 785Combined sources
Helixi79 – 813Combined sources
Helixi84 – 929Combined sources
Helixi93 – 953Combined sources
Helixi98 – 1025Combined sources
Helixi105 – 1128Combined sources
Helixi119 – 13416Combined sources
Beta strandi138 – 1414Combined sources
Helixi144 – 1463Combined sources
Helixi147 – 16418Combined sources
Beta strandi172 – 1743Combined sources
Beta strandi187 – 1893Combined sources
Helixi192 – 1943Combined sources
Beta strandi199 – 2013Combined sources
Helixi204 – 2129Combined sources
Helixi227 – 2293Combined sources
Helixi231 – 2366Combined sources
Helixi237 – 2393Combined sources
Helixi242 – 25413Combined sources
Helixi259 – 27315Combined sources
Helixi275 – 28612Combined sources
Helixi288 – 31730Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3M5WX-ray2.32A/B1-319[»]
3TZLX-ray2.15A/B1-319[»]
ProteinModelPortaliQ9PIB4.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9PIB4.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi9 – 179"HIGH" region
Motifi189 – 1935"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.
OrthoDBiEOG686NJQ.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PIB4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVLTGLQPS GDLHIGNYFG AIKQMVDAQE KSQMFMFIAN YHAMTSSQDG
60 70 80 90 100
EKLKQNSLKA AAAFLSLGID PQKSVFWLQS DVKEVMELYW ILSQFTPMGL
110 120 130 140 150
LERAHSYKDK VAKGLSASHG LFSYPVLMAA DILLFDTRIV PVGKDQIQHV
160 170 180 190 200
EIARDIALKV NNEWGEIFTL PEARVNEEVA VVVGTDGAKM SKSYQNTIDI
210 220 230 240 250
FSSEKTLKKQ ISSIVTDSTA LEDPKDHENC NIFKIAKLFL DESGQKELQI
260 270 280 290 300
RYEKGGEGYG HFKIYLNELV NAYFKEAREK YNELLEKPSH LKEILDFGAT
310
KARKIAQEKM QKIYEKIGL
Length:319
Mass (Da):36,084
Last modified:October 1, 2000 - v1
Checksum:iCB620B2B67AA6666
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34538.1.
PIRiB81382.
RefSeqiYP_002343825.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34538; CAL34538; Cj0388.
GeneIDi904711.
KEGGicje:Cj0388.
PATRICi20057691. VBICamJej33762_0379.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34538.1.
PIRiB81382.
RefSeqiYP_002343825.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3M5WX-ray2.32A/B1-319[»]
3TZLX-ray2.15A/B1-319[»]
ProteinModelPortaliQ9PIB4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PIB4. 3 interactions.
STRINGi192222.Cj0388.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34538; CAL34538; Cj0388.
GeneIDi904711.
KEGGicje:Cj0388.
PATRICi20057691. VBICamJej33762_0379.

Phylogenomic databases

eggNOGiCOG0180.
HOGENOMiHOG000059939.
KOiK01867.
OMAiWYLSCFF.
OrthoDBiEOG686NJQ.

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-364-MONOMER.

Miscellaneous databases

EvolutionaryTraceiQ9PIB4.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00140_B. Trp_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERiPTHR10055. PTHR10055. 1 hit.
PfamiPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSiPR01039. TRNASYNTHTRP.
TIGRFAMsiTIGR00233. trpS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiSYW_CAMJE
AccessioniPrimary (citable) accession number: Q9PIB4
Secondary accession number(s): Q0PBC2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2000
Last modified: January 7, 2015
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.