Reviewed, UniProtKB/Swiss-Prot Q9PI73 (MURD_CAMJE)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoylalanine--D-glutamate ligase
    EC=6.3.2.9
Alternative name(s):
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase
    D-glutamic acid-adding enzyme

Gene names

Name:	murD
Ordered Locus Names:	Cj0432c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	402 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity.
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the murCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 402	402	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000108989
Regions
	Nucleotide binding	97 – 103	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PI73-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 21889399DB049720	FASTA	402	45,329
	10 20 30 40 50 60 MKISLFGYGK TTRAIAENLV DKFGPFDIYD DHFTETKKDT LGNLLLNPND FDDNLSDIEI 70 80 90 100 110 120 PSPGFPPKHK LIQKAKNLQS EYDFFYDIMP KSVWISGTNG KTTTTQMATH LLSHIGAVMG 130 140 150 160 170 180 GNVGTPLAEL NPYAKLWILE TSSFTLHYTH KAKPEIYALL PISPDHLSWH GSFDNYVQDK 190 200 210 220 230 240 LSVLKRMNEC DVVILPKIYA NTPTKAHKIS YEDEKDLAVK FGIDTEKISF KSPFLLDAIM 250 260 270 280 290 300 ALAIEKILLD TLSYKLLNSF VMEKNKLEEL KDSQNRLWVN DTKATNESAV MAALSRYKDK 310 320 330 340 350 360 KIHLIIGGDD KGVDLSNLFD FMKGFDIELY AIGISTEKML DYAKKANLKA YKCEVLSKAV 370 380 390 400 NEISNHLRVN EVALLSPACA SLDQFNSYAE RGKVFKECVN KI

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34582.1.
PIR	F81387.
RefSeq	YP_002343869.1.
3D structure databases
HSSP	HSSP built from PDB template 2UAG based on UniProtKB P14900.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PI73. 38 interactions.
Genome annotation databases
GeneID	904757.
GenomeReviews	Gene locus Cj0432c in contig AL111168_GR.
KEGG	cje:Cj0432c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PI73. LWVNDTK.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0432C-MON.
BRENDA	6.3.2.9. 255835.
Family and domain databases
HAMAP	MF_00639. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01087. murD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CAMJE

Accession

Primary (citable) accession number: Q9PI73
Secondary accession number(s): Q0PB78

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)