Q9PI08 (HEMH_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ferrochelatase
EC=4.99.1.1

Alternative name(s):
Heme synthase
Protoheme ferro-lyase

Gene names

Name:	hemH
Ordered Locus Names:	Cj0503c

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the ferrous insertion into protoporphyrin IX. HAMAP MF_00323
Catalytic activity	Protoheme + 2 H+ = protoporphyrin + Fe2+. HAMAP MF_00323
Pathway	Porphyrin metabolism; protoheme biosynthesis; protoheme from protoporphyrin-IX: step 1/1. HAMAP MF_00323
Subcellular location	Cytoplasm By similarity HAMAP MF_00323.
Sequence similarities	Belongs to the ferrochelatase family.

Ontologies

Keywords
Biological process	Heme biosynthesis Porphyrin biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	heme biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ferrochelatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Ferrochelatase HAMAP MF_00323		PRO_0000175125
Sites
	Metal binding	185	1	Iron By similarity
	Metal binding	262	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PI08 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 3F7C864A5B833C50	FASTA	303	35,202
	10 20 30 40 50 60 MKLVLFLNMG GATNLQDCEV FLKNMFNDPY ILGIKNRFLR KFVAWIITKA RVKAMQENYK 70 80 90 100 110 120 KMGGKSPLNE LTQSLCDKLN LKQDEFKFDF VNLYVPPFAT EILQKYTLNA SDEIILFPLY 130 140 150 160 170 180 PHHSCTTVTS SLEVLQNEIS KQKIQAKVKT IDIFYKNELY NEMIVSHILA KKSKFDAKIL 190 200 210 220 230 240 IFSAHSLPQS IIDKGDLYEK HVNDHVEILK EKLKDHFDEF ILAYQSKLGP VKWLEPNTSD 250 260 270 280 290 300 VLANLNDKAL IYPISFCIDC SETIFELGME YKHLSKYNYD LISCPNDSDE FMEFILKYLS DLN

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34650.1.
PIR	H81395.
RefSeq	YP_002343935.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PI08.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PI08. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904832.
GenomeReviews	Gene locus Cj0503c in contig AL111168_GR.
KEGG	cje:Cj0503c.
PATRIC	20057948. VBICamJej33762_0496.
Phylogenomic databases
HOGENOM	HBG697135.
OMA	CSETIFE.
ProtClustDB	PRK00035.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0503C-MONOMER.
Family and domain databases
HAMAP	MF_00323. Ferrochelatase. [Tree]
InterPro	IPR001015. Ferrochelatase. IPR019772. Ferrochelatase_AS. [Graphical view]
KO	K01772.
PANTHER	PTHR11108. Ferrochelatase. 1 hit.
Pfam	PF00762. Ferrochelatase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00109. HemH. 1 hit.
PROSITE	PS00534. FERROCHELATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HEMH_CAMJE

Accession

Primary (citable) accession number: Q9PI08
Secondary accession number(s): Q0PB12

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program