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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi549ZincUniRule annotation1
Metal bindingi553ZincUniRule annotation1
Metal bindingi650ZincUniRule annotation1
Metal bindingi654ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase, RNA-binding, tRNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:G1G1F-486-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:Cj0506
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000750831 – 842Alanine--tRNA ligaseAdd BLAST842

Proteomic databases

PaxDbiQ9PI05
PRIDEiQ9PI05

Interactioni

Protein-protein interaction databases

STRINGi192222.Cj0506

Structurei

3D structure databases

ProteinModelPortaliQ9PI05
SMRiQ9PI05
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CIM Bacteria
COG0013 LUCA
HOGENOMiHOG000156965
KOiK01872
OMAiFEMMAHH

Family and domain databases

HAMAPiMF_00036_B Ala_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR002318 Ala-tRNA-lgiase_IIc
IPR018162 Ala-tRNA-ligase_IIc_anticod-bd
IPR018165 Ala-tRNA-synth_IIc_core
IPR018164 Ala-tRNA-synth_IIc_N
IPR023033 Ala_tRNA_ligase_euk/bac
IPR003156 DHHA1_dom
IPR018163 Thr/Ala-tRNA-synth_IIc_edit
IPR009000 Transl_B-barrel_sf
IPR012947 tRNA_SAD
PfamiView protein in Pfam
PF02272 DHHA1, 1 hit
PF01411 tRNA-synt_2c, 1 hit
PF07973 tRNA_SAD, 1 hit
PRINTSiPR00980 TRNASYNTHALA
SMARTiView protein in SMART
SM00863 tRNA_SAD, 1 hit
SUPFAMiSSF101353 SSF101353, 1 hit
SSF50447 SSF50447, 1 hit
SSF55186 SSF55186, 1 hit
TIGRFAMsiTIGR00344 alaS, 1 hit
PROSITEiView protein in PROSITE
PS50860 AA_TRNA_LIGASE_II_ALA, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PI05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP
60 70 80 90 100
RPNPPRKTSC QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE
110 120 130 140 150
QAIAYAWEFV TEVLKLPKDR LYVTVHENDD EAFNLWQKHI QKERIYKFGD
160 170 180 190 200
KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS SEDYMGGDGD RFLEIWNLVF
210 220 230 240 250
MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD SSLFMPIINE
260 270 280 290 300
ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL
310 320 330 340 350
RRALRHGYLL GFKQAFMYKL VDVVCDLMGG HYTYLNEKKD FIKEQIRLEE
360 370 380 390 400
ERFLSTIENG IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE
410 420 430 440 450
KNLKVDEEKF ELFMNEQKAR AKASWKGSGD KTASGDFKNL LEKFGENHFV
460 470 480 490 500
GYEKAECESK ILALLDEDFK EVSTLKDAGW VMLENTPFYA TSGGQSADSG
510 520 530 540 550
FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE KREQIARHHS
560 570 580 590 600
ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR
610 620 630 640 650
VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC
660 670 680 690 700
GGTHVKNTAQ IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK
710 720 730 740 750
VKDELKNNDI LSGIKKLKNE ILSLKNELKN SSKTELDSKN IQGVEICVKR
760 770 780 790 800
IDNGDIKAMI DDFKNKFAKA VILLIQVKDE KITLAAGVKD VPLKAGALVK
810 820 830 840
EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL
Length:842
Mass (Da):94,906
Last modified:October 1, 2000 - v1
Checksum:iF571EA5A2B60A8B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA Translation: CAL34653.1
PIRiC81396
RefSeqiWP_002858583.1, NC_002163.1
YP_002343938.1, NC_002163.1

Genome annotation databases

EnsemblBacteriaiCAL34653; CAL34653; Cj0506
GeneIDi904835
KEGGicje:Cj0506
PATRICifig|192222.6.peg.499

Similar proteinsi

Entry informationi

Entry nameiSYA_CAMJE
AccessioniPrimary (citable) accession number: Q9PI05
Secondary accession number(s): Q0PB09
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: May 23, 2018
This is version 99 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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