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Q9PI05

- SYA_CAMJE

UniProt

Q9PI05 - SYA_CAMJE

Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 82 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

    Catalytic activityi

    ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi549 – 5491ZincUniRule annotation
    Metal bindingi553 – 5531ZincUniRule annotation
    Metal bindingi650 – 6501ZincUniRule annotation
    Metal bindingi654 – 6541ZincUniRule annotation

    GO - Molecular functioni

    1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-HAMAP
    3. tRNA binding Source: UniProtKB-KW
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

    Enzyme and pathway databases

    BioCyciCJEJ192222:GJTS-491-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
    Alternative name(s):
    Alanyl-tRNA synthetaseUniRule annotation
    Short name:
    AlaRSUniRule annotation
    Gene namesi
    Name:alaSUniRule annotation
    Ordered Locus Names:Cj0506
    OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
    Taxonomic identifieri192222 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000000799: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 842842Alanine--tRNA ligasePRO_0000075083Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi192222.Cj0506.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9PI05.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

    Sequence similaritiesi

    Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0013.
    HOGENOMiHOG000156965.
    KOiK01872.
    OMAiYDIDIFQ.
    OrthoDBiEOG6Q2SQ2.

    Family and domain databases

    HAMAPiMF_00036_B. Ala_tRNA_synth_B.
    InterProiIPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view]
    PfamiPF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view]
    PRINTSiPR00980. TRNASYNTHALA.
    SMARTiSM00863. tRNA_SAD. 1 hit.
    [Graphical view]
    SUPFAMiSSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsiTIGR00344. alaS. 1 hit.
    PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9PI05-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP    50
    RPNPPRKTSC QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE 100
    QAIAYAWEFV TEVLKLPKDR LYVTVHENDD EAFNLWQKHI QKERIYKFGD 150
    KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS SEDYMGGDGD RFLEIWNLVF 200
    MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD SSLFMPIINE 250
    ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL 300
    RRALRHGYLL GFKQAFMYKL VDVVCDLMGG HYTYLNEKKD FIKEQIRLEE 350
    ERFLSTIENG IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE 400
    KNLKVDEEKF ELFMNEQKAR AKASWKGSGD KTASGDFKNL LEKFGENHFV 450
    GYEKAECESK ILALLDEDFK EVSTLKDAGW VMLENTPFYA TSGGQSADSG 500
    FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE KREQIARHHS 550
    ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR 600
    VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC 650
    GGTHVKNTAQ IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK 700
    VKDELKNNDI LSGIKKLKNE ILSLKNELKN SSKTELDSKN IQGVEICVKR 750
    IDNGDIKAMI DDFKNKFAKA VILLIQVKDE KITLAAGVKD VPLKAGALVK 800
    EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL 842
    Length:842
    Mass (Da):94,906
    Last modified:October 1, 2000 - v1
    Checksum:iF571EA5A2B60A8B7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL111168 Genomic DNA. Translation: CAL34653.1.
    PIRiC81396.
    RefSeqiYP_002343938.1. NC_002163.1.

    Genome annotation databases

    EnsemblBacteriaiCAL34653; CAL34653; Cj0506.
    GeneIDi904835.
    KEGGicje:Cj0506.
    PATRICi20057954. VBICamJej33762_0499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL111168 Genomic DNA. Translation: CAL34653.1 .
    PIRi C81396.
    RefSeqi YP_002343938.1. NC_002163.1.

    3D structure databases

    ProteinModelPortali Q9PI05.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 192222.Cj0506.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAL34653 ; CAL34653 ; Cj0506 .
    GeneIDi 904835.
    KEGGi cje:Cj0506.
    PATRICi 20057954. VBICamJej33762_0499.

    Phylogenomic databases

    eggNOGi COG0013.
    HOGENOMi HOG000156965.
    KOi K01872.
    OMAi YDIDIFQ.
    OrthoDBi EOG6Q2SQ2.

    Enzyme and pathway databases

    BioCyci CJEJ192222:GJTS-491-MONOMER.

    Family and domain databases

    HAMAPi MF_00036_B. Ala_tRNA_synth_B.
    InterProi IPR002318. Ala-tRNA-lgiase_IIc.
    IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
    IPR018165. Ala-tRNA-synth_IIc_core.
    IPR018164. Ala-tRNA-synth_IIc_N.
    IPR023033. Ala_tRNA_ligase_euk/bac.
    IPR003156. DHHA1_dom.
    IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
    IPR009000. Transl_B-barrel.
    IPR012947. tRNA_SAD.
    [Graphical view ]
    Pfami PF02272. DHHA1. 1 hit.
    PF01411. tRNA-synt_2c. 1 hit.
    PF07973. tRNA_SAD. 1 hit.
    [Graphical view ]
    PRINTSi PR00980. TRNASYNTHALA.
    SMARTi SM00863. tRNA_SAD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF101353. SSF101353. 1 hit.
    SSF50447. SSF50447. 1 hit.
    SSF55186. SSF55186. 1 hit.
    TIGRFAMsi TIGR00344. alaS. 1 hit.
    PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: NCTC 11168.

    Entry informationi

    Entry nameiSYA_CAMJE
    AccessioniPrimary (citable) accession number: Q9PI05
    Secondary accession number(s): Q0PB09
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 10, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 82 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3