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Q9PI05

- SYA_CAMJE

UniProt

Q9PI05 - SYA_CAMJE

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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi549 – 5491ZincUniRule annotation
Metal bindingi553 – 5531ZincUniRule annotation
Metal bindingi650 – 6501ZincUniRule annotation
Metal bindingi654 – 6541ZincUniRule annotation

GO - Molecular functioni

  1. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-491-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:Cj0506
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Alanine--tRNA ligasePRO_0000075083Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192222.Cj0506.

Structurei

3D structure databases

ProteinModelPortaliQ9PI05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiYDIDIFQ.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PI05-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP
60 70 80 90 100
RPNPPRKTSC QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE
110 120 130 140 150
QAIAYAWEFV TEVLKLPKDR LYVTVHENDD EAFNLWQKHI QKERIYKFGD
160 170 180 190 200
KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS SEDYMGGDGD RFLEIWNLVF
210 220 230 240 250
MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD SSLFMPIINE
260 270 280 290 300
ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL
310 320 330 340 350
RRALRHGYLL GFKQAFMYKL VDVVCDLMGG HYTYLNEKKD FIKEQIRLEE
360 370 380 390 400
ERFLSTIENG IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE
410 420 430 440 450
KNLKVDEEKF ELFMNEQKAR AKASWKGSGD KTASGDFKNL LEKFGENHFV
460 470 480 490 500
GYEKAECESK ILALLDEDFK EVSTLKDAGW VMLENTPFYA TSGGQSADSG
510 520 530 540 550
FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE KREQIARHHS
560 570 580 590 600
ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR
610 620 630 640 650
VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC
660 670 680 690 700
GGTHVKNTAQ IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK
710 720 730 740 750
VKDELKNNDI LSGIKKLKNE ILSLKNELKN SSKTELDSKN IQGVEICVKR
760 770 780 790 800
IDNGDIKAMI DDFKNKFAKA VILLIQVKDE KITLAAGVKD VPLKAGALVK
810 820 830 840
EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL
Length:842
Mass (Da):94,906
Last modified:October 1, 2000 - v1
Checksum:iF571EA5A2B60A8B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL34653.1.
PIRiC81396.
RefSeqiYP_002343938.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34653; CAL34653; Cj0506.
GeneIDi904835.
KEGGicje:Cj0506.
PATRICi20057954. VBICamJej33762_0499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL34653.1 .
PIRi C81396.
RefSeqi YP_002343938.1. NC_002163.1.

3D structure databases

ProteinModelPortali Q9PI05.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192222.Cj0506.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL34653 ; CAL34653 ; Cj0506 .
GeneIDi 904835.
KEGGi cje:Cj0506.
PATRICi 20057954. VBICamJej33762_0499.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi YDIDIFQ.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci CJEJ192222:GJTS-491-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiSYA_CAMJE
AccessioniPrimary (citable) accession number: Q9PI05
Secondary accession number(s): Q0PB09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: October 29, 2014
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3