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Protein

Alanine--tRNA ligase

Gene

alaS

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi549 – 5491ZincUniRule annotation
Metal bindingi553 – 5531ZincUniRule annotation
Metal bindingi650 – 6501ZincUniRule annotation
Metal bindingi654 – 6541ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-491-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligaseUniRule annotation (EC:6.1.1.7UniRule annotation)
Alternative name(s):
Alanyl-tRNA synthetaseUniRule annotation
Short name:
AlaRSUniRule annotation
Gene namesi
Name:alaSUniRule annotation
Ordered Locus Names:Cj0506
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Alanine--tRNA ligasePRO_0000075083Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192222.Cj0506.

Structurei

3D structure databases

ProteinModelPortaliQ9PI05.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.UniRule annotation

Sequence similaritiesi

Belongs to the class-II aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiPCSEIHI.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PI05-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP
60 70 80 90 100
RPNPPRKTSC QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE
110 120 130 140 150
QAIAYAWEFV TEVLKLPKDR LYVTVHENDD EAFNLWQKHI QKERIYKFGD
160 170 180 190 200
KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS SEDYMGGDGD RFLEIWNLVF
210 220 230 240 250
MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD SSLFMPIINE
260 270 280 290 300
ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL
310 320 330 340 350
RRALRHGYLL GFKQAFMYKL VDVVCDLMGG HYTYLNEKKD FIKEQIRLEE
360 370 380 390 400
ERFLSTIENG IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE
410 420 430 440 450
KNLKVDEEKF ELFMNEQKAR AKASWKGSGD KTASGDFKNL LEKFGENHFV
460 470 480 490 500
GYEKAECESK ILALLDEDFK EVSTLKDAGW VMLENTPFYA TSGGQSADSG
510 520 530 540 550
FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE KREQIARHHS
560 570 580 590 600
ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR
610 620 630 640 650
VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC
660 670 680 690 700
GGTHVKNTAQ IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK
710 720 730 740 750
VKDELKNNDI LSGIKKLKNE ILSLKNELKN SSKTELDSKN IQGVEICVKR
760 770 780 790 800
IDNGDIKAMI DDFKNKFAKA VILLIQVKDE KITLAAGVKD VPLKAGALVK
810 820 830 840
EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL
Length:842
Mass (Da):94,906
Last modified:October 1, 2000 - v1
Checksum:iF571EA5A2B60A8B7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34653.1.
PIRiC81396.
RefSeqiWP_002858583.1. NC_002163.1.
YP_002343938.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34653; CAL34653; Cj0506.
GeneIDi904835.
KEGGicje:Cj0506.
PATRICi20057954. VBICamJej33762_0499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34653.1.
PIRiC81396.
RefSeqiWP_002858583.1. NC_002163.1.
YP_002343938.1. NC_002163.1.

3D structure databases

ProteinModelPortaliQ9PI05.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi192222.Cj0506.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34653; CAL34653; Cj0506.
GeneIDi904835.
KEGGicje:Cj0506.
PATRICi20057954. VBICamJej33762_0499.

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiPCSEIHI.
OrthoDBiEOG6Q2SQ2.

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-491-MONOMER.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiSYA_CAMJE
AccessioniPrimary (citable) accession number: Q9PI05
Secondary accession number(s): Q0PB09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.