SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q9PI05

- SYA_CAMJE

UniProt

Q9PI05 - SYA_CAMJE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Alanine--tRNA ligase
Gene
alaS, Cj0506
Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain By similarity.UniRule annotation

Catalytic activityi

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi549 – 5491Zinc Reviewed prediction
Metal bindingi553 – 5531Zinc Reviewed prediction
Metal bindingi650 – 6501Zinc Reviewed prediction
Metal bindingi654 – 6541Zinc Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. alanine-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: UniProtKB-KW
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. alanyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, RNA-binding, tRNA-binding, Zinc

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-491-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alanine--tRNA ligase (EC:6.1.1.7)
Alternative name(s):
Alanyl-tRNA synthetase
Short name:
AlaRS
Gene namesi
Name:alaS
Ordered Locus Names:Cj0506
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000000799: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 842842Alanine--tRNA ligaseUniRule annotation
PRO_0000075083Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi192222.Cj0506.

Structurei

3D structure databases

ProteinModelPortaliQ9PI05.

Family & Domainsi

Domaini

Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0013.
HOGENOMiHOG000156965.
KOiK01872.
OMAiYDIDIFQ.
OrthoDBiEOG6Q2SQ2.

Family and domain databases

HAMAPiMF_00036_B. Ala_tRNA_synth_B.
InterProiIPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view]
PfamiPF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view]
PRINTSiPR00980. TRNASYNTHALA.
SMARTiSM00863. tRNA_SAD. 1 hit.
[Graphical view]
SUPFAMiSSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsiTIGR00344. alaS. 1 hit.
PROSITEiPS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PI05-1 [UniParc]FASTAAdd to Basket

« Hide

MDIRKAYLDF FASKGHEITP SSPLVPDDAT LLFTNAGMVP FKSIFTGEIP    50
RPNPPRKTSC QTCIRAGGKH NDLDNVGYTA RHHTFFEMLG NFSFGDYFKE 100
QAIAYAWEFV TEVLKLPKDR LYVTVHENDD EAFNLWQKHI QKERIYKFGD 150
KDNFWQMGDT GPCGPCSEIF YDQGQEHFNS SEDYMGGDGD RFLEIWNLVF 200
MQYERSADGV LSPLPKPSID TGMGLERVTA IKEGKFSNFD SSLFMPIINE 250
ISKLCNKTYV YESGASFRVI ADHIRSSVFL LAQGVSFDKE GRGYVLRRIL 300
RRALRHGYLL GFKQAFMYKL VDVVCDLMGG HYTYLNEKKD FIKEQIRLEE 350
ERFLSTIENG IEIFNEELKN TKEIFSGEVA FKLYDTYGFP LDLTADMLRE 400
KNLKVDEEKF ELFMNEQKAR AKASWKGSGD KTASGDFKNL LEKFGENHFV 450
GYEKAECESK ILALLDEDFK EVSTLKDAGW VMLENTPFYA TSGGQSADSG 500
FIAKREVLDT QKFFNLNLSF IKAGEELKVN DIVHARIDTE KREQIARHHS 550
ATHLLHHALR EILGSHVSQA GSLVESNKLR FDFTHHKALN KEELESIEKR 600
VNKMIINSSE AILENMPLEE AKKSGAIALF NEKYQGNVRV LTLGESKELC 650
GGTHVKNTAQ IGSFYIVKES GVSAGVRRIE AVVSKAALEF VKNQLEELSK 700
VKDELKNNDI LSGIKKLKNE ILSLKNELKN SSKTELDSKN IQGVEICVKR 750
IDNGDIKAMI DDFKNKFAKA VILLIQVKDE KITLAAGVKD VPLKAGALVK 800
EAAQILGGNG GGRDDFATAG GKDLSKINEA LKQSLETIEK AL 842
Length:842
Mass (Da):94,906
Last modified:October 1, 2000 - v1
Checksum:iF571EA5A2B60A8B7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL34653.1.
PIRiC81396.
RefSeqiYP_002343938.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34653; CAL34653; Cj0506.
GeneIDi904835.
KEGGicje:Cj0506.
PATRICi20057954. VBICamJej33762_0499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL111168 Genomic DNA. Translation: CAL34653.1 .
PIRi C81396.
RefSeqi YP_002343938.1. NC_002163.1.

3D structure databases

ProteinModelPortali Q9PI05.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 192222.Cj0506.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAL34653 ; CAL34653 ; Cj0506 .
GeneIDi 904835.
KEGGi cje:Cj0506.
PATRICi 20057954. VBICamJej33762_0499.

Phylogenomic databases

eggNOGi COG0013.
HOGENOMi HOG000156965.
KOi K01872.
OMAi YDIDIFQ.
OrthoDBi EOG6Q2SQ2.

Enzyme and pathway databases

BioCyci CJEJ192222:GJTS-491-MONOMER.

Family and domain databases

HAMAPi MF_00036_B. Ala_tRNA_synth_B.
InterProi IPR002318. Ala-tRNA-lgiase_IIc.
IPR018162. Ala-tRNA-ligase_IIc_anticod-bd.
IPR018165. Ala-tRNA-synth_IIc_core.
IPR018164. Ala-tRNA-synth_IIc_N.
IPR023033. Ala_tRNA_ligase_euk/bac.
IPR003156. DHHA1_dom.
IPR018163. Thr/Ala-tRNA-synth_IIc_edit.
IPR009000. Transl_B-barrel.
IPR012947. tRNA_SAD.
[Graphical view ]
Pfami PF02272. DHHA1. 1 hit.
PF01411. tRNA-synt_2c. 1 hit.
PF07973. tRNA_SAD. 1 hit.
[Graphical view ]
PRINTSi PR00980. TRNASYNTHALA.
SMARTi SM00863. tRNA_SAD. 1 hit.
[Graphical view ]
SUPFAMi SSF101353. SSF101353. 1 hit.
SSF50447. SSF50447. 1 hit.
SSF55186. SSF55186. 1 hit.
TIGRFAMsi TIGR00344. alaS. 1 hit.
PROSITEi PS50860. AA_TRNA_LIGASE_II_ALA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: NCTC 11168.

Entry informationi

Entry nameiSYA_CAMJE
AccessioniPrimary (citable) accession number: Q9PI05
Secondary accession number(s): Q0PB09
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: October 1, 2000
Last modified: September 3, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi