Q9PHY2 (MDH_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable malate dehydrogenase
EC=1.1.1.37

Gene names

Name:	mdh
Ordered Locus Names:	Cj0532

Organism

Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168) [Reference proteome] [HAMAP]

Taxonomic identifier

192222 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter › Campylobacter jejuni › Campylobacter jejuni subsp. jejuni

Protein attributes

Sequence length	300 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487
Catalytic activity	(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487
Sequence similarities	Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-malate dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 300	300	Probable malate dehydrogenase HAMAP-Rule MF_00487		PRO_0000113445
Regions
	Nucleotide binding	7 – 12	6	NAD By similarity
	Nucleotide binding	117 – 119	3	NAD By similarity
Sites
	Active site	174	1	Proton acceptor By similarity
	Binding site	32	1	NAD By similarity
	Binding site	81	1	Substrate By similarity
	Binding site	87	1	Substrate By similarity
	Binding site	94	1	NAD By similarity
	Binding site	119	1	Substrate By similarity
	Binding site	150	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PHY2 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 45DABA8AAA648A9A	FASTA	300	33,513
	10 20 30 40 50 60 MKITVIGAGN VGSSVAYALI LREIANEIVL VDINEDLLYA KELELTQSIA ALNLNIDLLC 70 80 90 100 110 120 TKDYTHTKNS DIVLFSAGFA RKDGQSREEL LQLNTSIMLD CAKKIKDFTE DPLFIILTNP 130 140 150 160 170 180 VDFLLNTLYE SGIFSSKKII AMAGVLDNAR FKYELAKKLN VKMSRVDTRL IGFHNDDMVL 190 200 210 220 230 240 VKSYASVKNK NISEFLNEEE FEDLENEVKT GGAKVIKHLK TSAYLAPASA CIRMLESIRS 250 260 270 280 290 300 GEFLPMSVIL HGEFGVQNKA LGVMARLGLE GVIEIMKMDL SLQEKDKLEK SLIKYQYKGE

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34678.1.
PIR	D81399.
RefSeq	YP_002343963.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PHY2.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PHY2. 1 interaction.
STRING	192222.Cj0532.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	CAL34678; CAL34678; Cj0532.
GeneID	904860.
KEGG	cje:Cj0532.
PATRIC	20058004. VBICamJej33762_0524.
Phylogenomic databases
eggNOG	COG0039.
HOGENOM	HOG000213794.
KO	K00024.
OMA	NYKDIEG.
ProtClustDB	CLSK878824.
Enzyme and pathway databases
BioCyc	CJEJ192222:GJTS-516-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit. 3.90.110.10. 1 hit.
HAMAP	MF_00487. Malate_dehydrog_3. Divergent sequence.
InterPro	IPR001557. L-lactate/malate_DH. IPR022383. Lactate/malate_DH_C. IPR001236. Lactate/malate_DH_N. IPR015955. Lactate_DH/Glyco_Ohase_4_C. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11540. PTHR11540. 1 hit.
Pfam	PF02866. Ldh_1_C. 1 hit. PF00056. Ldh_1_N. 1 hit. [Graphical view]
PIRSF	PIRSF000102. Lac_mal_DH. 1 hit.
PRINTS	PR00086. LLDHDRGNASE.
SUPFAM	SSF56327. Lactate_DH/Glyco_hydro_4_C. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MDH_CAMJE

Accession

Primary (citable) accession number: Q9PHY2
Secondary accession number(s): Q0PAY4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 16, 2004
Last sequence update:	October 1, 2000
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program