Reviewed, UniProtKB/Swiss-Prot Q9PHU4 (RIBB_CAMJE)

Names and origin

Protein names

Recommended name:
    3,4-dihydroxy-2-butanone 4-phosphate synthase
      Short name=DHBP synthase
    EC=4.1.99.12

Gene names

Name:	ribB
Ordered Locus Names:	Cj0572

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate By similarity. HAMAP MF_00180
Catalytic activity	D-ribulose 5-phosphate = formate + L-3,4-dihydroxybutan-2-one 4-phosphate. HAMAP MF_00180
Cofactor	Binds 2 divalent metal cations per subunit. Magnesium or manganese By similarity. HAMAP MF_00180
Pathway	Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1. HAMAP MF_00180
Sequence similarities	In the N-terminal section; belongs to the DHBP synthase family. In the C-terminal section; belongs to the GTP cyclohydrolase II family.

Ontologies

Keywords
Biological process	Riboflavin biosynthesis
Ligand	Magnesium Manganese Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	riboflavin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3,4-dihydroxy-2-butanone-4-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP GTP cyclohydrolase II activity Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliG	Q0PBJ0	1	EBI-1195310,EBI-1191336

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	3,4-dihydroxy-2-butanone 4-phosphate synthase HAMAP MF_00180		PRO_0000151722
Regions
	Region	1 – 206	206	DHBP synthase HAMAP MF_00180
	Region	27 – 28	2	Substrate binding By similarity
	Region	139 – 143	5	Substrate binding By similarity
	Region	207 – 339	133	GTP cyclohydrolase II-like HAMAP MF_00180
Sites
	Metal binding	28	1	Magnesium or manganese 1 By similarity
	Metal binding	28	1	Magnesium or manganese 2 By similarity
	Metal binding	142	1	Magnesium or manganese 2 By similarity
	Binding site	32	1	Substrate By similarity
	Binding site	163	1	Substrate By similarity
	Site	125	1	Essential for catalytic activity By similarity
	Site	163	1	Essential for catalytic activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PHU4-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 6D90CF5F6A3F3DD9	FASTA	339	37,925
	10 20 30 40 50 60 MKFVSVEQAI KDLQAGKMLV MVDAEDRENE GDLIFPAQFS TQEKVNFMIK EARGVVCVAL 70 80 90 100 110 120 DETLAKKFEL PLMVPKNTSN HETAFTITVD AKDATTGVSA YERNMTIQIF ADDNAKASDF 130 140 150 160 170 180 VRPGHINPLI AKKGGVLERT GHTEGTVDLC KLAGLKGACV ICEIVKDNGD MARREDLEIF 190 200 210 220 230 240 CQKHDLNMIA VSDLIEYRLK HESLIKLEEK SQSVLAGFKA EKFIFSDHNQ TQHIAFCFKD 250 260 270 280 290 300 IKKCENVKFH ISGSDFELLT SDKFSKLLEQ IKFLSENGGV IVFMQGEKSS TTQYKNYGIG 310 320 330 AQILRYFGIE EIKLLSQSCD KDYIGLEGFG LNLKACNFN

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34718.1.
PIR	C81404.
RefSeq	YP_002344002.1.
3D structure databases
SMR	Q9PHU4. Positions 2-202, 217-334.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PHU4. 69 interactions.
Genome annotation databases
GeneID	904897.
GenomeReviews	Gene locus Cj0572 in contig AL111168_GR.
KEGG	cje:Cj0572.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG735778.
OMA	FAITHAK.
PhylomeDB	Q9PHU4.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0572-MONOMER.
BRENDA	4.1.99.12. 255835.
Family and domain databases
HAMAP	MF_00180. RibB. Fused. [Tree]
InterPro	IPR017945. DHBP_synth_RibB-like_a/b_dom. IPR000422. DHBP_synthase_RibB. IPR000926. GTP_CycHdrlase_II. IPR016299. Riboflavin_synth_RibA. [Graphical view]
Gene3D	G3DSA:3.90.870.10. DHBP_synth_RibB-like_a/b_dom. 1 hit.
Pfam	PF00926. DHBP_synthase. 1 hit. PF00925. GTP_cyclohydro2. 1 hit. [Graphical view]
PIRSF	PIRSF001259. RibA. 1 hit.
TIGRFAMs	TIGR00506. ribB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBB_CAMJE

Accession

Primary (citable) accession number: Q9PHU4
Secondary accession number(s): Q0PAU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	October 1, 2000
Last modified:	February 9, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)