Q9PHN5 (ILVC_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	Cj0632

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliY	Q0PC74	1	EBI-1194802,EBI-1191111

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_0000151297
Sites
	Active site	109	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9PHN5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: DA556ED90C86C780	FASTA	340	36,935
	10 20 30 40 50 60 MAITVYYDKD CDLNLIKSKK VAIIGFGSQG HAHAMNLRDN GVNVTIGLRE GSVSAVKAKN 70 80 90 100 110 120 AGFEVVSVSE ASKIADVIMI LAPDEIQADI FNVEIKPNLS EGKAIAFAHG FNIHYGQIVV 130 140 150 160 170 180 PKGVDVIMIA PKAPGHTVRN EFTLGGGTPC LIAIHQDESK NAKNLALSYA SAIGGGRTGI 190 200 210 220 230 240 IETTFKAETE TDLFGEQAVL CGGLSALIQA GFETLVEAGY EPEMAYFECL HEMKLIVDLI 250 260 270 280 290 300 YQGGIADMRY SISNTAEYGD YITGPKIITE ETKKAMKGVL KDIQNGVFAK DFILERRAGF 310 320 330 340 ARMHAERKNM NDSLIEKTGR NLRAMMPWIS AKKLVDKDKN

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34777.1.
PIR	G81411.
RefSeq	YP_002344061.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PHN5.
SMR	Q9PHN5. Positions 5-329.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PHN5. 43 interactions.
PTM databases
PhosSite	Q9PHN5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904961.
GenomeReviews	Gene locus Cj0632 in contig AL111168_GR.
KEGG	cje:Cj0632.
PATRIC	20058209. VBICamJej33762_0624.
Phylogenomic databases
HOGENOM	HBG286525.
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0632-MONOMER.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_CAMJE

Accession

Primary (citable) accession number: Q9PHN5
Secondary accession number(s): Q0PAN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 29, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program