Q9PHK9 (HSLV_CAMJE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent protease subunit HslV
EC=3.4.25.2

Gene names

Name:	hslV
Ordered Locus Names:	Cj0663c

Organism

Campylobacter jejuni

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease subunit of a proteasome-like degradation complex believed to be a general protein degrading machinery By similarity. HAMAP MF_00248
Catalytic activity	ATP-dependent cleavage of peptide bonds with broad specificity. HAMAP MF_00248
Enzyme regulation	Allosterically activated by HslU binding By similarity. HAMAP MF_00248
Subunit structure	A double ring-shaped homohexamer of HslV is capped on each side by a ring-shaped HslU homohexamer. The assembly of the HslU/HslV complex is dependent on binding of ATP By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00248.
Sequence similarities	Belongs to the peptidase T1B family. HslV subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding Sodium
Molecular function	Hydrolase Protease Threonine protease
Technical term	Allosteric enzyme Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	proteolysis involved in cellular protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	HslUV protease complex Inferred from electronic annotation. Source: InterPro proteasome core complex Inferred from electronic annotation. Source: InterPro
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 180	180	ATP-dependent protease subunit HslV HAMAP MF_00248		PRO_0000148099
Sites
	Active site	5	1	By similarity
	Metal binding	161	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	164	1	Sodium; via carbonyl oxygen By similarity
	Metal binding	167	1	Sodium; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PHK9 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 4FA4E4BA86A7D3F1	FASTA	180	19,592
	10 20 30 40 50 60 MFHATTILAY KGKNKSVIGG DGQVSFGNTV LKGNAVKIRK LNNGKVLAGF AGSTADAFNL 70 80 90 100 110 120 FDMFENLLQS SKGDLLKAAI DFSKEWRKDK YLRKLEAMML VLDRNHIFLL SGTGDVVEPE 130 140 150 160 170 180 DGQIAAIGSG GNYALSAARA LAKHTDLDEE ELVKSSLQIA GEICIYTNTN IKTYVIEDEK

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL111168 Genomic DNA. Translation: CAL34804.1.
PIR	A81415.
RefSeq	YP_002344087.1. NC_002163.1.
3D structure databases
ProteinModelPortal	Q9PHK9.
SMR	Q9PHK9. Positions 5-170.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PHK9. 90 interactions.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	904986.
GenomeReviews	Gene locus Cj0663c in contig AL111168_GR.
KEGG	cje:Cj0663c.
PATRIC	20058271. VBICamJej33762_0655.
Phylogenomic databases
HOGENOM	HBG288822.
OMA	WRTDKYL.
PhylomeDB	Q9PHK9.
ProtClustDB	PRK05456.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0663C-MONOMER.
Family and domain databases
HAMAP	MF_00248. HslV. [Tree]
InterPro	IPR022281. ATP-dep_Prtase_HsIV_su. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K01419.
PANTHER	PTHR11599:SF38. PTHR11599:SF38. 1 hit.
Pfam	PF00227. Proteasome. 1 hit. [Graphical view]
TIGRFAMs	TIGR03692. ATP_dep_HslV. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HSLV_CAMJE

Accession

Primary (citable) accession number: Q9PHK9
Secondary accession number(s): Q0PAL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program