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Protein

Argininosuccinate synthase

Gene

argG

Organism
Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate.UniRule annotation

Pathwayi: L-arginine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase (argF)
  2. Argininosuccinate synthase (argG)
  3. Argininosuccinate lyase (argH)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Binding sitei91CitrullineUniRule annotation1
Binding sitei96CitrullineUniRule annotation1
Binding sitei121ATP; via amide nitrogenUniRule annotation1
Binding sitei123AspartateUniRule annotation1
Binding sitei127AspartateUniRule annotation1
Binding sitei127CitrullineUniRule annotation1
Binding sitei128AspartateUniRule annotation1
Binding sitei131CitrullineUniRule annotation1
Binding sitei181CitrullineUniRule annotation1
Binding sitei190CitrullineUniRule annotation1
Binding sitei266CitrullineUniRule annotation1
Binding sitei278CitrullineUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 19ATPUniRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCJEJ192222:GJTS-624-MONOMER.
UniPathwayiUPA00068; UER00113.

Names & Taxonomyi

Protein namesi
Recommended name:
Argininosuccinate synthaseUniRule annotation (EC:6.3.4.5UniRule annotation)
Alternative name(s):
Citrulline--aspartate ligaseUniRule annotation
Gene namesi
Name:argGUniRule annotation
Ordered Locus Names:Cj0665c
OrganismiCampylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC 11168)
Taxonomic identifieri192222 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
Proteomesi
  • UP000000799 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001485801 – 406Argininosuccinate synthaseAdd BLAST406

Proteomic databases

PaxDbiQ9PHK7.

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Protein-protein interaction databases

IntActiQ9PHK7. 42 interactors.
STRINGi192222.Cj0665c.

Structurei

Secondary structure

1406
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi7 – 11Combined sources5
Helixi16 – 28Combined sources13
Beta strandi32 – 41Combined sources10
Helixi47 – 55Combined sources9
Helixi60 – 62Combined sources3
Beta strandi63 – 67Combined sources5
Helixi69 – 75Combined sources7
Helixi77 – 81Combined sources5
Turni82 – 84Combined sources3
Turni88 – 90Combined sources3
Helixi94 – 113Combined sources20
Beta strandi116 – 119Combined sources4
Helixi128 – 139Combined sources12
Beta strandi144 – 146Combined sources3
Helixi148 – 151Combined sources4
Helixi158 – 166Combined sources9
Beta strandi179 – 184Combined sources6
Beta strandi189 – 193Combined sources5
Helixi194 – 197Combined sources4
Helixi205 – 207Combined sources3
Turni214 – 216Combined sources3
Beta strandi222 – 229Combined sources8
Beta strandi232 – 236Combined sources5
Helixi243 – 256Combined sources14
Beta strandi261 – 267Combined sources7
Beta strandi269 – 279Combined sources11
Helixi281 – 297Combined sources17
Helixi300 – 319Combined sources20
Beta strandi322 – 324Combined sources3
Helixi325 – 336Combined sources12
Turni337 – 340Combined sources4
Beta strandi343 – 350Combined sources8
Beta strandi353 – 360Combined sources8
Helixi382 – 401Combined sources20

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NZPX-ray2.31A1-406[»]
ProteinModelPortaliQ9PHK7.
SMRiQ9PHK7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the argininosuccinate synthase family. Type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PHK7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKNEVKKVVL AYSGGLDTSI ILKWLQDEYN CEVVTFTADI GQGEELEPAR
60 70 80 90 100
KKALSLGIKE ENIFIKDLRD EFVKDYVFPM FRANAIYEGE YLLGTSIARP
110 120 130 140 150
LIAKTQAQIA LQTGADAVSH GATGKGNDQV RFELGYLAFS PDLKIIAPWR
160 170 180 190 200
EWDLNSREKL LAYAQKHGID ISKKKGKSPY SMDANLLHIS YEGLVLEDPA
210 220 230 240 250
HAPEEDMWRW SKSPKDAPNE SEIIELDFQK GDLVAINGEK LSPAGLLTKL
260 270 280 290 300
NELGCKHGIG RLDIVENRYV GMKSRGCYET PGGTILLKAH RALESITLDR
310 320 330 340 350
EAAHLKDELM PKYASLIYNG YWFSPERMML QALIDESQIH ANGRVKLELY
360 370 380 390 400
KGNVMVIGRE SANDSLFNAA YCTFEEDEVY NQKDAAGFIK LNALRFIIAG

KNGRKF
Length:406
Mass (Da):45,578
Last modified:October 1, 2000 - v1
Checksum:i8A1E137AF30EC77F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34806.1.
PIRiC81415.
RefSeqiWP_002852200.1. NC_002163.1.
YP_002344089.1. NC_002163.1.

Genome annotation databases

EnsemblBacteriaiCAL34806; CAL34806; Cj0665c.
GeneIDi904988.
KEGGicje:Cj0665c.
PATRICi20058275. VBICamJej33762_0657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL111168 Genomic DNA. Translation: CAL34806.1.
PIRiC81415.
RefSeqiWP_002852200.1. NC_002163.1.
YP_002344089.1. NC_002163.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4NZPX-ray2.31A1-406[»]
ProteinModelPortaliQ9PHK7.
SMRiQ9PHK7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9PHK7. 42 interactors.
STRINGi192222.Cj0665c.

Proteomic databases

PaxDbiQ9PHK7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAL34806; CAL34806; Cj0665c.
GeneIDi904988.
KEGGicje:Cj0665c.
PATRICi20058275. VBICamJej33762_0657.

Phylogenomic databases

eggNOGiENOG4105CDH. Bacteria.
COG0137. LUCA.
HOGENOMiHOG000230093.
KOiK01940.
OMAiQCEVVTF.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00113.
BioCyciCJEJ192222:GJTS-624-MONOMER.

Family and domain databases

CDDicd01999. Argininosuccinate_Synthase. 1 hit.
Gene3Di3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPiMF_00005. Arg_succ_synth_type1. 1 hit.
InterProiIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00032. argG. 1 hit.
PROSITEiPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASSY_CAMJE
AccessioniPrimary (citable) accession number: Q9PHK7
Secondary accession number(s): Q0PAK8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 23, 2003
Last sequence update: October 1, 2000
Last modified: November 2, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.