Reviewed, UniProtKB/Swiss-Prot Q9PHK7 (ASSY_CAMJE)

Names and origin

Protein names

Recommended name:
    Argininosuccinate synthase
    EC=6.3.4.5
Alternative name(s):
    Citrulline--aspartate ligase

Gene names

Name:	argG
Ordered Locus Names:	Cj0665c

Organism

Campylobacter jejuni [Complete proteome] [HAMAP]

Taxonomic identifier

197 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP MF_00005
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP MF_00005
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP argininosuccinate synthase activity Inferred from electronic annotation. Source: HAMAP protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
fliM	Q0PC73	1	EBI-1195029,EBI-1191095
fliN	O32370	1	EBI-1195029,EBI-1191320

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 406	406	Argininosuccinate synthase HAMAP MF_00005		PRO_0000148580
Regions
	Nucleotide binding	11 – 19	9	ATP By similarity
Sites
	Binding site	38	1	ATP; via amide nitrogen and carbonyl oxygen By similarity
	Binding site	91	1	Citrulline By similarity
	Binding site	96	1	Citrulline By similarity
	Binding site	121	1	ATP; via amide nitrogen By similarity
	Binding site	123	1	Aspartate By similarity
	Binding site	127	1	Aspartate By similarity
	Binding site	127	1	Citrulline By similarity
	Binding site	128	1	Aspartate By similarity
	Binding site	131	1	Citrulline By similarity
	Binding site	181	1	Citrulline By similarity
	Binding site	190	1	Citrulline By similarity
	Binding site	266	1	Citrulline By similarity
	Binding site	278	1	Citrulline By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PHK7-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8A1E137AF30EC77F	FASTA	406	45,578
	10 20 30 40 50 60 MKNEVKKVVL AYSGGLDTSI ILKWLQDEYN CEVVTFTADI GQGEELEPAR KKALSLGIKE 70 80 90 100 110 120 ENIFIKDLRD EFVKDYVFPM FRANAIYEGE YLLGTSIARP LIAKTQAQIA LQTGADAVSH 130 140 150 160 170 180 GATGKGNDQV RFELGYLAFS PDLKIIAPWR EWDLNSREKL LAYAQKHGID ISKKKGKSPY 190 200 210 220 230 240 SMDANLLHIS YEGLVLEDPA HAPEEDMWRW SKSPKDAPNE SEIIELDFQK GDLVAINGEK 250 260 270 280 290 300 LSPAGLLTKL NELGCKHGIG RLDIVENRYV GMKSRGCYET PGGTILLKAH RALESITLDR 310 320 330 340 350 360 EAAHLKDELM PKYASLIYNG YWFSPERMML QALIDESQIH ANGRVKLELY KGNVMVIGRE 370 380 390 400 SANDSLFNAA YCTFEEDEVY NQKDAAGFIK LNALRFIIAG KNGRKF

References

[1]

"The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences." Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M., Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S., Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A., Rajandream M.A., Rutherford K.M. , van Vliet A.H.M., Whitehead S., Barrell B.G. Nature 403:665-668(2000) [PubMed: 10688204] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: NCTC 11168 / Serotype O:2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AL111168 Genomic DNA. Translation: CAL34806.1.
PIR	C81415.
RefSeq	YP_002344089.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9PHK7. 43 interactions.
Genome annotation databases
GeneID	904988.
GenomeReviews	Gene locus Cj0665c in contig AL111168_GR.
KEGG	cje:Cj0665c.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	Q9PHK7. YVFPMFR.
Enzyme and pathway databases
BioCyc	CJEJ192222:CJ0665C-MON.
BRENDA	6.3.4.5. 255835.
Family and domain databases
HAMAP	MF_00005. [Tree]
InterPro	IPR001518. Arginosuc_synth. IPR018223. Arginosuc_synth_CS. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR11587. Arginosuc_synth. 1 hit.
Pfam	PF00764. Arginosuc_synth. 1 hit. [Graphical view]
TIGRFAMs	TIGR00032. argG. 1 hit.
PROSITE	PS00564. ARGININOSUCCIN_SYN_1. 1 hit. PS00565. ARGININOSUCCIN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASSY_CAMJE

Accession

Primary (citable) accession number: Q9PHK7
Secondary accession number(s): Q0PAK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)