Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Probable cytosol aminopeptidase

Gene

pepA

Organism
Xylella fastidiosa (strain 9a5c)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides.UniRule annotation

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.UniRule annotation
Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 2 manganese ions per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi264 – 2641Manganese 2UniRule annotation
Metal bindingi269 – 2691Manganese 1UniRule annotation
Metal bindingi269 – 2691Manganese 2UniRule annotation
Active sitei276 – 2761UniRule annotation
Metal bindingi287 – 2871Manganese 2UniRule annotation
Metal bindingi346 – 3461Manganese 1UniRule annotation
Metal bindingi348 – 3481Manganese 1UniRule annotation
Metal bindingi348 – 3481Manganese 2UniRule annotation
Active sitei350 – 3501UniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Aminopeptidase, Hydrolase, Protease

Keywords - Ligandi

Manganese, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable cytosol aminopeptidaseUniRule annotation (EC:3.4.11.1UniRule annotation)
Alternative name(s):
Leucine aminopeptidaseUniRule annotation (EC:3.4.11.10UniRule annotation)
Short name:
LAPUniRule annotation
Leucyl aminopeptidaseUniRule annotation
Gene namesi
Name:pepAUniRule annotation
Ordered Locus Names:XF_0138
OrganismiXylella fastidiosa (strain 9a5c)
Taxonomic identifieri160492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella
ProteomesiUP000000812 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Probable cytosol aminopeptidasePRO_0000165818Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi160492.XF0138.

Structurei

3D structure databases

ProteinModelPortaliQ9PH08.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0260.
KOiK01255.
OMAiTAWRMPL.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9PH08-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALQFQLNQT TPQTVTTDCV IVGIYADKTL SPTAKTLDAA SGGRITALTA
60 70 80 90 100
RGDLTGKSGT SALLHDLNGV TAPRVLVVGL GEADKFGPGQ YIKAVGDAVR
110 120 130 140 150
ALKDAPVTHA LLTLSELPVK DRNAAWNIHQ AVIAADHAAY RYTATLGTSR
160 170 180 190 200
KKAAESGLIT LAIHGQESSG LTLGQAIAEG VEYARALGNL PPNICTPVYL
210 220 230 240 250
AETTAHFAAT HPGATCEILD ESKMEALGMG ALLAVARGSA NRPRLIVLKW
260 270 280 290 300
NGGGDARPYV LVGKGITFDT GGVNLKTQGG IEEMKYDMCG GAAVIGTFVA
310 320 330 340 350
AVKVRLPLNL IVIVPAVENA IDGNAYRPSD VITSMSGKTI EVGNTDAEGR
360 370 380 390 400
LILCDALTYA ERFKPEALID VATLTGACMI ALGRAATGLM THHDDLANEL
410 420 430 440 450
LTAGEHVHDR AWRLPLWDEY QNLLDSTFAD VYNIGGRWGG AITAGCFLSR
460 470 480 490
FTEGQRWAHL DIAGSASNEG KRGMATGRPV GLLTQWLVDR C
Length:491
Mass (Da):51,723
Last modified:October 1, 2000 - v1
Checksum:i3D0576F8A7CAF3EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003849 Genomic DNA. Translation: AAF82951.1.
PIRiF82843.
RefSeqiNP_297431.1. NC_002488.3.
WP_010892683.1. NC_002488.3.

Genome annotation databases

EnsemblBacteriaiAAF82951; AAF82951; XF_0138.
GeneIDi1125656.
KEGGixfa:XF0138.
PATRICi24130178. VBIXylFas578_0145.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003849 Genomic DNA. Translation: AAF82951.1.
PIRiF82843.
RefSeqiNP_297431.1. NC_002488.3.
WP_010892683.1. NC_002488.3.

3D structure databases

ProteinModelPortaliQ9PH08.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi160492.XF0138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAF82951; AAF82951; XF_0138.
GeneIDi1125656.
KEGGixfa:XF0138.
PATRICi24130178. VBIXylFas578_0145.

Phylogenomic databases

eggNOGiCOG0260.
KOiK01255.
OMAiTAWRMPL.
OrthoDBiEOG6FV8B3.

Family and domain databases

HAMAPiMF_00181. Cytosol_peptidase_M17.
InterProiIPR011356. Leucine_aapep/pepB.
IPR000819. Peptidase_M17_C.
IPR023042. Peptidase_M17_leu_NH2_pept.
IPR008283. Peptidase_M17_N.
[Graphical view]
PfamiPF00883. Peptidase_M17. 1 hit.
PF02789. Peptidase_M17_N. 1 hit.
[Graphical view]
PRINTSiPR00481. LAMNOPPTDASE.
PROSITEiPS00631. CYTOSOL_AP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of the plant pathogen Xylella fastidiosa."
    Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A.
    , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.
    Nature 406:151-159(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 9a5c.

Entry informationi

Entry nameiAMPA_XYLFA
AccessioniPrimary (citable) accession number: Q9PH08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 8, 2002
Last sequence update: October 1, 2000
Last modified: May 27, 2015
This is version 91 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.