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Q9PGI7 (NUOI_XYLFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 84. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
NADH-quinone oxidoreductase subunit I
EC=1.6.99.5
Alternative name(s):
NADH dehydrogenase I subunit I
NDH-1 subunit I
Gene names
Name:nuoI
Ordered Locus Names:XF_0313
OrganismXylella fastidiosa [Complete proteome] [HAMAP]
Taxonomic identifier2371 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length163 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient By similarity. HAMAP MF_01351

Catalytic activity

NADH + quinone = NAD+ + quinol. HAMAP MF_01351

Cofactor

Binds 2 4Fe-4S clusters per subunit By similarity. HAMAP MF_01351

Subunit structure

NDH-1 is composed of 14 different subunits. Subunits NuoA, H, J, K, L, M, N constitute the membrane sector of the complex By similarity.

Subcellular location

Cell inner membrane; Peripheral membrane protein Potential HAMAP MF_01351.

Sequence similarities

Belongs to the complex I 23 kDa subunit family.

Contains 2 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 163163NADH-quinone oxidoreductase subunit I HAMAP MF_01351
PRO_0000250956

Regions

Domain54 – 84314Fe-4S ferredoxin-type 1
Domain94 – 123304Fe-4S ferredoxin-type 2

Sites

Metal binding641Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding671Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding701Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding741Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1031Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1061Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1091Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1131Iron-sulfur 1 (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PGI7 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FAC9A36C4527C948

FASTA16318,990
        10         20         30         40         50         60 
MMNRVMHYFK SLLLLELLAG LWLTLKYTFR PKYTVLYPME KFPQSPRFRG LHALRRYPNG 

        70         80         90        100        110        120 
EERCIACKLC EAVCPALAIT IDSAKREDGT RRTTRYDIDL FKCIFCGFCE ESCPVDSIVE 

       130        140        150        160 
THILEYHFEK RGENIVTKPQ LLAIGDRFEA EIAERRAADA AFR

« Hide

References

[1]"The genome sequence of the plant pathogen Xylella fastidiosa."
Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. expand/collapse author list , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.
Nature 406:151-159(2000) [PubMed: 10910347] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9a5c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003849 Genomic DNA. Translation: AAF83124.1.
PIRC82822.
RefSeqNP_297604.1. NC_002488.3.

3D structure databases

HSSPHSSP built from PDB template 2FDN based on UniProtKB P00198.
ProteinModelPortalQ9PGI7.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1125841.
GenomeReviewsGene locus XF_0313 in contig AE003849_GR.
KEGGxfa:XF0313.
PATRIC24130589. VBIXylFas578_0341.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG724359.
OMACPAMAIT.
ProtClustDBPRK05888.

Enzyme and pathway databases

BioCycXFAS160492:XF0313-MONOMER.

Family and domain databases

HAMAPMF_01351. NDH1_NuoI.
[Tree]
InterProIPR001450. 4Fe4S-bd_dom.
IPR017896. 4Fe4S_Fe-S-bd.
IPR017900. 4Fe4S_Fe_S_CS.
IPR012285. Fum_reductase_C.
IPR010226. NADH_quinone_OxRdtase_chainI.
[Graphical view]
Gene3DG3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
KOK00338.
PfamPF12838. Fer4_7. 1 hit.
[Graphical view]
TIGRFAMsTIGR01971. NuoI. 1 hit.
PROSITEPS00198. 4FE4S_FER_1. 2 hits.
PS51379. 4FE4S_FER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNUOI_XYLFA
AccessionPrimary (citable) accession number: Q9PGI7
Entry history
Integrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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