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Q9PFD6 (SYFB_XYLFA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phenylalanine--tRNA ligase beta chain
EC=6.1.1.20
Alternative name(s):
Phenylalanyl-tRNA synthetase beta chain
Short name=PheRS
Gene names
Name:pheT
Ordered Locus Names:XF_0742
OrganismXylella fastidiosa [Complete proteome] [HAMAP]
Taxonomic identifier2371 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length792 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00283

Cofactor

Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00283

Subunit structure

Tetramer of two alpha and two beta chains By similarity.

Subcellular location

Cytoplasm HAMAP MF_00283.

Sequence similarities

Belongs to the phenylalanyl-tRNA synthetase beta chain family. Type 1 subfamily.

Contains 1 B5 domain.

Contains 1 FDX-ACB domain.

Contains 1 tRNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 792792Phenylalanine--tRNA ligase beta chain HAMAP MF_00283
PRO_0000126991

Regions

Domain39 – 147109tRNA-binding
Domain400 – 47576B5
Domain698 – 79194FDX-ACB

Sites

Metal binding4531Magnesium By similarity
Metal binding4591Magnesium; via carbonyl oxygen By similarity
Metal binding4621Magnesium By similarity
Metal binding4631Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PFD6 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 99D39C9860398B37

FASTA79286,233
        10         20         30         40         50         60 
MKFSENWLRN HVPIQANRDV LVATLTAIGL EVENVAVLGE ALDLIVVARI VNVVPHPESD 

        70         80         90        100        110        120 
RLQICQVDAA QDTLLQIVCG ASNVRPGLVV PLALLGAKIG ALTIKSTTLR GVESNGMLCS 

       130        140        150        160        170        180 
AKELGLDTEA SGLMELPDDA PIGTPLADYL ALPDASIEIK LTPNRADCFS VRGIAFDVAA 

       190        200        210        220        230        240 
ACASEVTPLH IDEIPAVSAR TLPVELHAGA NAPRYCGCVI EGIDPAAPTP VWMAERLRRS 

       250        260        270        280        290        300 
GIRPVSLLVD ITQYVMLELG QPMHAFDVDT LRGPIGVRLS RNDEALKLLD GRTVVLDNDF 

       310        320        330        340        350        360 
LVVTDADQPI ALAGLIGGWE TRITDTTVNV FLEAAHFAPV AIMGRGRKLG LHTDASHRFE 

       370        380        390        400        410        420 
RGVDPALPPQ AIAFATRLIL ELAGGKPGSL IRVELPEYLP APASILLRRT RIARLLGIVI 

       430        440        450        460        470        480 
DDVEVERILH ALGMQVTTQA EGWRVVAPSR RFDIAIEEDL IEELVRIRGY EHLPTALPIG 

       490        500        510        520        530        540 
ASHIAMPSET RLDMTSVRRQ LIARELQETI NYAFIDAELL RRWQLNTGQV MLMNPLSAEL 

       550        560        570        580        590        600 
AVIRPRLLPG LVAALGRNIA RQLERVRLFE LGNVFTASDE AGAAPLETQH VAAAVCGDAF 

       610        620        630        640        650        660 
ALQWGEQARK VDFYDLKGDL ESLAAASGAV LTFHSSAQPW GHPGRSADVW CDDTCIGWIG 

       670        680        690        700        710        720 
QLHPALTQTL EINVDVIAFE LALEPLVRGA LPRAHALSRF PFVRRDLACV VPEHVTWSEL 

       730        740        750        760        770        780 
AITVRDVIGS LLRDVKLFDR YVGKGIEPGF KSLAIGLILQ DDTRTLIDRD VDDIMAKVVM 

       790 
AIQQRHDVRI RS

« Hide

References

[1]"The genome sequence of the plant pathogen Xylella fastidiosa."
Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. expand/collapse author list , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.
Nature 406:151-159(2000) [PubMed: 10910347] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9a5c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AE003849 Genomic DNA. Translation: AAF83552.1.
PIRC82767.
RefSeqNP_298032.1. NC_002488.3.

3D structure databases

ProteinModelPortalQ9PFD6.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1126275.
GenomeReviewsGene locus XF_0742 in contig AE003849_GR.
KEGGxfa:XF0742.
NMPDRfig|160492.1.peg.739.
PATRIC24131481. VBIXylFas578_0782.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHBG399638.
OMAADKLRVC.
ProtClustDBPRK00629.

Enzyme and pathway databases

BioCycXFAS160492:XF0742-MONOMER.

Family and domain databases

HAMAPMF_00283. Phe_tRNA_synth_beta1.
[Tree]
InterProIPR005146. B3/B4_tRNA-bd.
IPR009061. DNA-bd_dom_put.
IPR012340. NA-bd_OB-fold.
IPR016027. NA-bd_OB-fold-like.
IPR004532. Phe-tRNA-synth_IIc_bsu_bac.
IPR020825. Phe-tRNA_synthase_B3/B4.
IPR005121. PheS_beta_Fdx_antiC-bd.
IPR002547. tRNA-bd_dom.
IPR005147. tRNA_synthase_B5-dom.
[Graphical view]
Gene3DG3DSA:3.50.40.10. B3_4. 1 hit.
G3DSA:3.30.56.20. B5. 1 hit.
G3DSA:3.30.70.380. Fdx_AntiC_bd. 1 hit.
G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KOK01890.
PfamPF03483. B3_4. 1 hit.
PF03484. B5. 1 hit.
PF03147. FDX-ACB. 1 hit.
PF01588. tRNA_bind. 1 hit.
[Graphical view]
SMARTSM00873. B3_4. 1 hit.
SM00874. B5. 1 hit.
SM00896. FDX-ACB. 1 hit.
[Graphical view]
SUPFAMSSF56037. B3_4. 1 hit.
SSF54991. Fdx_AntiC_bd. 1 hit.
SSF50249. Nucleic_acid_OB. 1 hit.
SSF46955. Putativ_DNA_bind. 1 hit.
TIGRFAMsTIGR00472. PheT_bact. 1 hit.
PROSITEPS51483. B5. 1 hit.
PS51447. FDX_ACB. 1 hit.
PS50886. TRBD. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYFB_XYLFA
AccessionPrimary (citable) accession number: Q9PFD6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 8, 2000
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

SIMILARITY comments

Index of protein domains and families

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