Q9PEV1 (FMT_XYLFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Methionyl-tRNA formyltransferase
EC=2.1.2.9

Gene names

Name:	fmt
Ordered Locus Names:	XF_0927

Organism

Xylella fastidiosa [Complete proteome] [HAMAP]

Taxonomic identifier

2371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by: (I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-GTP By similarity. HAMAP MF_00182
Catalytic activity	10-formyltetrahydrofolate + L-methionyl-tRNA(fMet) + H2O = tetrahydrofolate + N-formylmethionyl-tRNA(fMet). HAMAP MF_00182
Sequence similarities	Belongs to the fmt family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	methionyl-tRNA formyltransferase activity Inferred from electronic annotation. Source: EC methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	Methionyl-tRNA formyltransferase HAMAP MF_00182		PRO_0000083091
Regions
	Region	108 – 111	4	Tetrahydrofolate (THF) binding By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PEV1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 06DC17F40FA5A8F7	FASTA	307	33,237
	10 20 30 40 50 60 MRIVFAGTPD FAVPSLRSVT QRADVVAVYT QPDRPAGRGR ELTPSPVKLE AVARGLPVYQ 70 80 90 100 110 120 PQTLRSPEML EQLRALRPDL IVVVAYGVIL PEAVLAIPDD GCWNVHASLL PRWRGAAPIQ 130 140 150 160 170 180 RAIEAGDTET GVCLMQMEAG LDTGPVLMSL KTPINAYETS GQLHDRLAEM GAQLLSDGLG 190 200 210 220 230 240 LLRAGLRPVP QPQLAAGVTY AHKLGKVEAQ LDWEQPAERL ACRVRAFQPW PVAEVVLCGE 250 260 270 280 290 300 RVRIHEALAL DLDHSQPPGT VLAASKEGID VACLQGALRL CRLQREGGKA ITAADYLNAR RDLQVRA

References

[1]

"The genome sequence of the plant pathogen Xylella fastidiosa." Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C. Nature 406:151-159(2000) [PubMed: 10910347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9a5c.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003849 Genomic DNA. Translation: AAF83737.1.
PIR	H82743.
RefSeq	NP_298217.1. NC_002488.3.
3D structure databases
ProteinModelPortal	Q9PEV1.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1126463.
GenomeReviews	Gene locus XF_0927 in contig AE003849_GR.
KEGG	xfa:XF0927.
NMPDR	fig|160492.1.peg.924.
PATRIC	24131908. VBIXylFas578_0993.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571560.
OMA	IMQMDEG.
ProtClustDB	PRK00005.
Enzyme and pathway databases
BioCyc	XFAS160492:XF0927-MONOMER.
Family and domain databases
HAMAP	MF_00182. Formyl_trans. [Tree]
InterPro	IPR005794. Fmt. IPR005793. Formyl_trans_C. IPR002376. Formyl_transf_N. IPR011034. Formyl_transferase_C-like. IPR001555. GART_AS. IPR015518. Met_tRNA_Form_TA-like. [Graphical view]
Gene3D	G3DSA:3.10.25.10. Formyl_trans_C. 1 hit. G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
KO	K00604.
PANTHER	PTHR11138. Met_tRNA_Form_TA-like. 1 hit.
Pfam	PF02911. Formyl_trans_C. 1 hit. PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF50486. FMT_C_like. 1 hit. SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00460. Fmt. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FMT_XYLFA

Accession

Primary (citable) accession number: Q9PEV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2002
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program