Q9PEN0 (AOTC_XYLFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
N-acetylornithine carbamoyltransferase
Short name=AOTCase
EC=2.1.3.9

Gene names

Name:	argF'
Ordered Locus Names:	XF_0998

Organism

Xylella fastidiosa [Complete proteome] [HAMAP]

Taxonomic identifier

2371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length	336 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the conversion of N-acetylornithine to N-acetylcitrulline in an alternative arginine biosynthesis pathway. The enzyme has no activity with ornithine By similarity.
Catalytic activity	Carbamoyl phosphate + N(2)-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline.
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the ATCase/OTCase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylornithine carbamoyltransferase activity Inferred from electronic annotation. Source: EC amino acid binding Inferred from electronic annotation. Source: InterPro aspartate carbamoyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 336	336	N-acetylornithine carbamoyltransferase		PRO_0000113269
Regions
	Region	49 – 53	5	Carbamoyl phosphate binding By similarity
	Region	293 – 296	4	N-acetylornithine binding By similarity
Sites
	Binding site	112	1	Carbamoyl phosphate By similarity
	Binding site	148	1	Carbamoyl phosphate By similarity
	Site	28	1	Important for structural integrity By similarity
	Site	161	1	Important for structural integrity By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PEN0 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 5B909F441AA37438	FASTA	336	37,623
	10 20 30 40 50 60 MALKHFLNTQ DWSCSELNAL LTQARAFKHN KLGNGLKGKS IALVFFNASM RTRSSFELGA 70 80 90 100 110 120 FQLGGHAIVL QPGKDAWPIE FDTGTVMEAE TEEHICEVAR VLGHYVDLIG VRAFPKFLDW 130 140 150 160 170 180 TYDRQDIVLN GFAKYSPVPV INMETITHPC QELAHIMALQ EHFGTTDLRG KKYVLTWTYH 190 200 210 220 230 240 PKPLNTAVAN SALTIATRLG MDVTLLCPTP DYVLDERYID WAQQNIADTG STFQVSHDID 250 260 270 280 290 300 NAYRGADVIY AKSWGALPFF GNWAMEKPIR DQYRHFIVDE AKMALTNNAV FSHCLPLRRN 310 320 330 VKATDAVMDG PNCIAIHEAG NRLHVQKAIM AALASQ

References

[1]

"The genome sequence of the plant pathogen Xylella fastidiosa." Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C. Nature 406:151-159(2000) [PubMed: 10910347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9a5c.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003849 Genomic DNA. Translation: AAF83808.1.
PIR	F82737.
RefSeq	NP_298288.1. NC_002488.3.
3D structure databases
ProteinModelPortal	Q9PEN0.
SMR	Q9PEN0. Positions 3-336.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1126535.
GenomeReviews	Gene locus XF_0998 in contig AE003849_GR.
KEGG	xfa:XF0998.
NMPDR	fig|160492.1.peg.995.
PATRIC	24132060. VBIXylFas578_1068.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG579429.
OMA	ITHPEGY.
ProtClustDB	PRK04523.
Enzyme and pathway databases
BioCyc	XFAS160492:XF0998-MONOMER.
Family and domain databases
InterPro	IPR006132. Asp/Orn_carbamoyltranf_P-bd. IPR006130. Asp/Orn_carbamoylTrfase. IPR006131. Asp_carbamoyltransf_Asp/Orn-bd. IPR002082. Asp_carbamoyltransf_euk. [Graphical view]
KO	K09065.
Pfam	PF00185. OTCace. 1 hit. PF02729. OTCace_N. 1 hit. [Graphical view]
PRINTS	PR00100. AOTCASE. PR00101. ATCASE.
SUPFAM	SSF53671. Asp/Orn_carbamoyltranf. 1 hit.
PROSITE	PS00097. CARBAMOYLTRANSFERASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AOTC_XYLFA

Accession

Primary (citable) accession number: Q9PEN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 11, 2003
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program