Q9PDF6 (PUR4_XYLFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase
Short name=FGAM synthase
Short name=FGAMS
EC=6.3.5.3

Alternative name(s):
Formylglycinamide ribotide amidotransferase
Short name=FGARAT
Formylglycinamide ribotide synthetase

Gene names

Name:	purL
Ordered Locus Names:	XF_1423

Organism

Xylella fastidiosa [Complete proteome] [HAMAP]

Taxonomic identifier

2371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length	1322 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00419
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00419
Subunit structure	Monomer By similarity. HAMAP MF_00419
Subcellular location	Cytoplasm By similarity HAMAP MF_00419.
Sequence similarities	In the N-terminal section; belongs to the FGAMS family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1322	1322	Phosphoribosylformylglycinamidine synthase HAMAP MF_00419		PRO_0000100426
Regions
	Domain	1073 – 1322	250	Glutamine amidotransferase type-1
	Nucleotide binding	300 – 311	12	ATP Potential
Sites
	Active site	1166	1	Nucleophile By similarity
	Active site	1287	1	By similarity
	Active site	1289	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PDF6 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: A066AC4E956BFA4C	FASTA	1,322	143,721
	10 20 30 40 50 60 MIVLKGASAL SPFRLARFES RLQTTVPELH IVGAWHCYLI QIKSGHTLDM SALHRILQAE 70 80 90 100 110 120 SVSEPPQEHV VSRYVMPRLG THSPWSSKTT ELLHGAGQPI ARIERGTRID LLGWSANAAT 130 140 150 160 170 180 CPAIAKQLYD PMTQSLLESE DEVKTLFNVP EPLSLERIAL IDLEHANTRW GLALTADEID 190 200 210 220 230 240 YLRTRYTELN RVPSDVELMM FAQANSEHCR HKIFNATWTI NDKEQQHSLF QMIKHTHQHT 250 260 270 280 290 300 PQYTLSAYAD NAAVIEGHPT ARYRPDPITG EYRHEAVLPG AFQIKVETHN HPTAIAPFPG 310 320 330 340 350 360 ASTGAGGEIR DEGATGRGGK PKAGLSGFSV SHLRIPTLPH PWETPRALNP RMASALDIML 370 380 390 400 410 420 EGPLGSAAFN NEFGRPNLLG YFRSFELSAS KTLTRAYDKP IMLAGGLGAI DRIHIKKLRL 430 440 450 460 470 480 QPGDVIIVLG GPAMLIGLGG GAASSVTSGT STEALDFASV QRDNPEMQRR CQEVIDHCVA 490 500 510 520 530 540 LGTNNPIRSF HDVGAGGLSN AIPELLHDSE VGGVIDLAKI PSDDPSLSPL ELWCNESQER 550 560 570 580 590 600 YVLGISAAHL QAFAAICTRE RCPFAAVGVA TATEQLIVGY GVTLPAALHV TEQQTPQRAN 610 620 630 640 650 660 HTETSPTPNT LPPSVREAFA IDLPMDVLFG KAPKMHRNTA HPPPPHWPTL DSTQLDLHHA 670 680 690 700 710 720 GLRVLAHPTV AAKNFLVTIG DRSIGGLTAR EQMIGPWQLP LADCAITLAG FGTYAGEAFA 730 740 750 760 770 780 IGERAPLALL NSAAAARMAV GEAITNLCAA PVESLSMVKL SANWMAAAEH PGEDALLYDA 790 800 810 820 830 840 VKAIGIELCP ALDISIPVGK DSLSMQSRWQ ADGATHTCIS PVSLVISAFT PVADARRQLT 850 860 870 880 890 900 PLLHHQTNSE LWLIALDGGK QRLGGSVLAQ VHADSAALPT FGGECPDLDT PETLRAFFAL 910 920 930 940 950 960 MNDARNAGLL LAYHDRSDGG AFAALCEMAF ASHLGLDITC DNRTEHLFPH LFNEELGAIV 970 980 990 1000 1010 1020 QVADEHRTAF TDLVEQHGLT AYTQRIAHPT TAPSIRIMHN DQCLAQWTWE TLFDAWWSVT 1030 1040 1050 1060 1070 1080 HAMQRLRDNP ECADEEREIA RTFTAPGLKP TLSFDPAVDV AMPFIATGIR PTVAILREQG 1090 1100 1110 1120 1130 1140 INGHIEMALC FERAGFHCVD IHMNDLITGR VHLDEFVGLA ACGGFSYGDV LGAGRGWATS 1150 1160 1170 1180 1190 1200 ILERTALRDQ FAAFFTRTDR FALGVCNGCQ MLSQLKSMIP GAEHWPRFVR NRSEQFEART 1210 1220 1230 1240 1250 1260 ALLEVIQSPS IFLSGMAGSR LPVAVAHGEG YAMFDTPADQ AAAHVALRYI NGHGQAATHY 1270 1280 1290 1300 1310 1320 PLNPNGSPNG ITGLTTTDGR ITILMPHPER TPRTINLSWS PNEWGEDTPW LRLFRNARAW VG

References

[1]

"The genome sequence of the plant pathogen Xylella fastidiosa." Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C. Nature 406:151-159(2000) [PubMed: 10910347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9a5c.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE003849 Genomic DNA. Translation: AAF84232.1.
PIR	D82685.
RefSeq	NP_298712.1. NC_002488.3.
3D structure databases
ProteinModelPortal	Q9PDF6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1126969.
GenomeReviews	Gene locus XF_1423 in contig AE003849_GR.
KEGG	xfa:XF1423.
NMPDR	fig|160492.1.peg.1419.
PATRIC	24132948. VBIXylFas578_1502.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG335309.
OMA	VKAVGME.
ProtClustDB	PRK05297.
Enzyme and pathway databases
BioCyc	XFAS160492:XF1423-MONOMER.
Family and domain databases
HAMAP	MF_00419. PurL_1. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR017926. GATASE_1. IPR010073. PRibForGlyAmidine_synth. IPR022940. PRibForGlyAmidine_synth_bac. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01735. FGAM_synt. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR4_XYLFA

Accession

Primary (citable) accession number: Q9PDF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	October 1, 2000
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program