Reviewed, UniProtKB/Swiss-Prot Q9PC29 (GSHB_XYLFA)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase

Gene names

Name:	gshB
Ordered Locus Names:	XF_1956

Organism

Xylella fastidiosa [Complete proteome] [HAMAP]

Taxonomic identifier

2371 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xylella

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Glutathione synthetase HAMAP MF_00162		PRO_0000197498
Regions
	Domain	125 – 311	187	ATP-grasp
	Nucleotide binding	151 – 208	58	ATP By similarity
Sites
	Metal binding	282	1	Magnesium or manganese By similarity
	Metal binding	284	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9PC29-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D05F3E281E50F8D9	FASTA	314	34,714
	10 20 30 40 50 60 MPLDVVVVMD PITGIKIAKD TTFALLLEGQ RRSHRLHYVH PGSLSLNEGR TFAQIAPLQV 70 80 90 100 110 120 RDNKTDWYTL GEFSETQLGQ GQIILMRKDP PVNAEFIYDT QLLSIAQAAG AQVINHPQGL 130 140 150 160 170 180 RDLNEKLAAQ LFPQCCPPTL ISRDMRALKM FVQKQEQAIL KPLDGMGGHS IFRSSNGDPN 190 200 210 220 230 240 LNVILETLTD GGRTLAIAQR YLQQIIEGDK RILLIDGVPI DHCLARIPQG DEFRGNMAAG 250 260 270 280 290 300 GRGESRPLNE HDRWIAAQVG PEMRRRGMRF VGIDVIGDYL TEINVTSPTC LRELDAQCGL 310 NIAGQLFDAI ETGG

References

[1]

"The genome sequence of the plant pathogen Xylella fastidiosa." Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C. Nature 406:151-159(2000) [PubMed: 10910347] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 9a5c.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AE003849 Genomic DNA. Translation: AAF84758.1.
PIR	F82616.
RefSeq	NP_299238.1.
3D structure databases
HSSP	HSSP built from PDB template 1GLV based on UniProtKB P04425.
ModBase	Search...
Genome annotation databases
GeneID	1127507.
GenomeReviews	Gene locus XF_1956 in contig AE003849_GR.
KEGG	xfa:XF1956.
NMPDR	fig|160492.1.peg.1945.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9PC29.
OMA	Q9PC29. VRNAPEK.
Enzyme and pathway databases
BioCyc	XFAS160492:XF1956-MON.
BRENDA	6.3.2.3. 278708.
Family and domain databases
HAMAP	MF_00162. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP_bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_XYLFA

Accession

Primary (citable) accession number: Q9PC29

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 23, 2002
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)