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Q9PBG8 (SYL_XYLFA) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:XF_2176
OrganismXylella fastidiosa (strain 9a5c) [Complete proteome] [HAMAP]
Taxonomic identifier160492 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella

Protein attributes

Sequence length879 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Sequence caution

The sequence AAF84975.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 879879Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000152122

Regions

Motif45 – 5511"HIGH" region HAMAP-Rule MF_00049
Motif637 – 6415"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6401ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9PBG8 [UniParc].

Last modified June 1, 2001. Version 2.
Checksum: 9FDCCB992092919E

FASTA87999,797
        10         20         30         40         50         60 
MPTEANTYDP QRIESTAQHY WDSTHAFEVN EHSNKPKYYC LSMLPYPSGA LHMGHVRNYT 

        70         80         90        100        110        120 
IGDVISRYKR MTGHNVLQPM GWDAFGLPAE NAAIKNKVAP AQWTYKNIER MRTQLKSLGY 

       130        140        150        160        170        180 
AIDWSREFAT CQPDYYVHEQ HMFTRLMRKG LAYRRNALVN WDPVDQTVLA NEQVIDGRGW 

       190        200        210        220        230        240 
RSGAPVEKRE IPQWFLRITD YAQELLDGLN TLDDWPEPVK TMQRNWIGRS EGLEIQFEVR 

       250        260        270        280        290        300 
DADNNALEAL RVFTTRPDTL LGVTFVSIAA EHPLALHAAK SNPALAGLLT EMKQGGLSEA 

       310        320        330        340        350        360 
ELKTQEKRGM DTGLKAIHPI TNEQLPVWVA NFVLMAYGTG AVMAVPGHDQ RDQEFANKYG 

       370        380        390        400        410        420 
LPIRQVIALK EPKNQDESTW EPDVWRDWYA DKTREFELIN SAEFDGLDYQ DAFEVLAERF 

       430        440        450        460        470        480 
ERQGRGQRRV NYRLRDWGVS RQRYWGCPIP VIYCPTCGAV PVPEDQLPVI LPENVAFSGT 

       490        500        510        520        530        540 
GSPIKTDPEW RKTTCPECGG PAERETDTFD TFMESSWYYA RYTSPNAHEM LDKRANYWLP 

       550        560        570        580        590        600 
VDQYIGGIEH AILHLMYFRF YHKLMRDARL VDSDEPAINL LTQGMVIAET FYRKNPDGSK 

       610        620        630        640        650        660 
DWINPADVNV ECDERGRITG ATLISDGQPV LIGATEKMSK SKNNGVDPQI MVTKYGADTV 

       670        680        690        700        710        720 
RLFSMFAAPP EQSLEWNEAG VEGMARFLRR LWTQVHHHAA HGPATALDIT ALDTAQKAMR 

       730        740        750        760        770        780 
CKTHNTIARV EDDYGRRRSF NTAIAAVMEL SNTLARFDDT TTQSHAVRQE ALETMVLLLN 

       790        800        810        820        830        840 
PITPHTSHAL WQTLGHPETL LEDLPFPKID TTALVRETTT LAVQVNGKLR GTIEVATDAP 

       850        860        870 
REHIENNART EPNTARFLEG LTVLKIIIVP GKIVNIVAR 

« Hide

References

[1]"The genome sequence of the plant pathogen Xylella fastidiosa."
Simpson A.J.G., Reinach F.C., Arruda P., Abreu F.A., Acencio M., Alvarenga R., Alves L.M.C., Araya J.E., Baia G.S., Baptista C.S., Barros M.H., Bonaccorsi E.D., Bordin S., Bove J.M., Briones M.R.S., Bueno M.R.P., Camargo A.A., Camargo L.E.A. expand/collapse author list , Carraro D.M., Carrer H., Colauto N.B., Colombo C., Costa F.F., Costa M.C.R., Costa-Neto C.M., Coutinho L.L., Cristofani M., Dias-Neto E., Docena C., El-Dorry H., Facincani A.P., Ferreira A.J.S., Ferreira V.C.A., Ferro J.A., Fraga J.S., Franca S.C., Franco M.C., Frohme M., Furlan L.R., Garnier M., Goldman G.H., Goldman M.H.S., Gomes S.L., Gruber A., Ho P.L., Hoheisel J.D., Junqueira M.L., Kemper E.L., Kitajima J.P., Krieger J.E., Kuramae E.E., Laigret F., Lambais M.R., Leite L.C.C., Lemos E.G.M., Lemos M.V.F., Lopes S.A., Lopes C.R., Machado J.A., Machado M.A., Madeira A.M.B.N., Madeira H.M.F., Marino C.L., Marques M.V., Martins E.A.L., Martins E.M.F., Matsukuma A.Y., Menck C.F.M., Miracca E.C., Miyaki C.Y., Monteiro-Vitorello C.B., Moon D.H., Nagai M.A., Nascimento A.L.T.O., Netto L.E.S., Nhani A. Jr., Nobrega F.G., Nunes L.R., Oliveira M.A., de Oliveira M.C., de Oliveira R.C., Palmieri D.A., Paris A., Peixoto B.R., Pereira G.A.G., Pereira H.A. Jr., Pesquero J.B., Quaggio R.B., Roberto P.G., Rodrigues V., de Rosa A.J.M., de Rosa V.E. Jr., de Sa R.G., Santelli R.V., Sawasaki H.E., da Silva A.C.R., da Silva A.M., da Silva F.R., Silva W.A. Jr., da Silveira J.F., Silvestri M.L.Z., Siqueira W.J., de Souza A.A., de Souza A.P., Terenzi M.F., Truffi D., Tsai S.M., Tsuhako M.H., Vallada H., Van Sluys M.A., Verjovski-Almeida S., Vettore A.L., Zago M.A., Zatz M., Meidanis J., Setubal J.C.
Nature 406:151-159(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 9a5c.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE003849 Genomic DNA. Translation: AAF84975.1. Different initiation.
PIRE82590.
RefSeqNP_299455.1. NC_002488.3.

3D structure databases

ProteinModelPortalQ9PBG8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING160492.XF2176.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAF84975; AAF84975; XF_2176.
GeneID1127729.
KEGGxfa:XF2176.
PATRIC24134592. VBIXylFas578_2316.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_XYLFA
AccessionPrimary (citable) accession number: Q9PBG8
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: June 1, 2001
Last modified: April 16, 2014
This is version 85 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries