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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Xylella fastidiosa (strain 9a5c)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei577Aminoacyl-adenylateUniRule annotation1
Binding sitei621ATPUniRule annotation1
Metal bindingi906ZincUniRule annotation1
Metal bindingi909ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:XF_2418
OrganismiXylella fastidiosa (strain 9a5c)
Taxonomic identifieri160492 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXylella
Proteomesi
  • UP000000812 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000985091 – 943Isoleucine--tRNA ligaseAdd BLAST943

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi160492.XF2418

Structurei

3D structure databases

ProteinModelPortaliQ9PAS7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi59 – 69"HIGH" regionAdd BLAST11
Motifi618 – 622"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9PAS7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSQDYKTTLH LPATDFPMRG DLPKREPAIL ERWERDDFYA QLRAHAKGRP
60 70 80 90 100
LFLLHDGPPY ANGQIHLGHA VNKILKDIII KSKHLDGFDA PYIPGWDCHG
110 120 130 140 150
LPIEIAIEKK YGKVGVTLDA VQFRQKCREY AAEQIQLQRR DFKRLGIIGD
160 170 180 190 200
WDAPYKTLDF RFEADEIRAL AKIVDKGHLI RGTKPVHWCF DCGSALAEAE
210 220 230 240 250
IEYTDKTSPM VDVAYPALDP SALAAVFNAT LPPDVQLAVP IWTTTPWTLP
260 270 280 290 300
ASLAISVGPT LDYVLVEGPT HSGQRRWLIL AEALAAKALA RYGIAELLIH
310 320 330 340 350
GSAKGAAMEQ HILAHPFYPD RTIPLLLGNH VSAEDGTGAV HTAPGHGQED
360 370 380 390 400
HQVFQQYGLL NHYSAAELNP VDARGVYLPT TPPFSELTLA GLHIWKANPL
410 420 430 440 450
IVDALRLRGV LLAAAEMHHS YPHCWRHKTP IVFRATPQWF ISMEQAALRS
460 470 480 490 500
AALKAITHVT WYPQWGQARI LSMIENRPDW TISRQRTWGV PIPLFVHRHS
510 520 530 540 550
GAPHPRSAAL MRQVADRVEQ QGVDIWYSLD QTELLGTEAD QYEKITDILD
560 570 580 590 600
VWFDSGITHE AVLLERGLPK PADLYLEGAD QHRGWFQSSL LTGVAMDNAA
610 620 630 640 650
PYKQCLTHGF TVDQHGRKMS KSLGNGIEPQ DIIKTLGADI LRLWIASTDY
660 670 680 690 700
SNEMSLSQEI LKRTTDAYRR IRNTARFLLG NLHGFDPTLH LVPLSDMIAL
710 720 730 740 750
DRWIVHRAFE LQQTIKAAYT RYDFAEIVQT ILNFCSVDLG SLYLDVTKDR
760 770 780 790 800
LYTMREDAPG RRSAQTAMYH LTAAFVRWIA PILSFTADEL WSYLPGDHAD
810 820 830 840 850
NVLFTTWYDG LAPLPPNAPL TAADFDKLLT LRDHVTKVLE PMRANGVIGA
860 870 880 890 900
ALEAEITIAA DADTAARWQP LTEELRFLFI SGDVTVTPAN TDGFFVSAQP
910 920 930 940
TTKAKCARCW HYRADIGAHP THPELCGRCV TNVDGPGEQR HWF
Length:943
Mass (Da):105,688
Last modified:October 1, 2000 - v1
Checksum:iD5AE18BF211AC081
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE003849 Genomic DNA Translation: AAF85217.1
PIRiC82559
RefSeqiWP_010894863.1, NC_002488.3

Genome annotation databases

EnsemblBacteriaiAAF85217; AAF85217; XF_2418
GeneIDi1127973
KEGGixfa:XF_2418

Similar proteinsi

Entry informationi

Entry nameiSYI_XYLFA
AccessioniPrimary (citable) accession number: Q9PAS7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: October 1, 2000
Last modified: March 28, 2018
This is version 104 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health