ID   PYRF_HALSA              Reviewed;         267 AA.
AC   Q9P9M3;
DT   23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT   24-JUN-2002, sequence version 3.
DT   27-MAR-2024, entry version 115.
DE   RecName: Full=Orotidine 5'-phosphate decarboxylase;
DE            EC=4.1.1.23;
DE   AltName: Full=OMP decarboxylase;
DE            Short=OMPDCase;
DE            Short=OMPdecase;
GN   Name=pyrF; Synonyms=ura3; OrderedLocusNames=VNG_1673G;
OS   Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1)
OS   (Halobacterium halobium).
OC   Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales;
OC   Halobacteriaceae; Halobacterium; Halobacterium salinarum NRC-34001.
OX   NCBI_TaxID=64091;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MPK1;
RX   PubMed=10672188; DOI=10.1046/j.1365-2958.2000.01739.x;
RA   Peck R.F., DasSarma S., Krebs M.P.;
RT   "Homologous gene knockout in the archaeon Halobacterium salinarum with ura3
RT   as a counterselectable marker.";
RL   Mol. Microbiol. 35:667-676(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700922 / JCM 11081 / NRC-1;
RX   PubMed=11016950; DOI=10.1073/pnas.190337797;
RA   Ng W.V., Kennedy S.P., Mahairas G.G., Berquist B., Pan M., Shukla H.D.,
RA   Lasky S.R., Baliga N.S., Thorsson V., Sbrogna J., Swartzell S., Weir D.,
RA   Hall J., Dahl T.A., Welti R., Goo Y.A., Leithauser B., Keller K., Cruz R.,
RA   Danson M.J., Hough D.W., Maddocks D.G., Jablonski P.E., Krebs M.P.,
RA   Angevine C.M., Dale H., Isenbarger T.A., Peck R.F., Pohlschroder M.,
RA   Spudich J.L., Jung K.-H., Alam M., Freitas T., Hou S., Daniels C.J.,
RA   Dennis P.P., Omer A.D., Ebhardt H., Lowe T.M., Liang P., Riley M., Hood L.,
RA   DasSarma S.;
RT   "Genome sequence of Halobacterium species NRC-1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:12176-12181(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H(+) + orotidine 5'-phosphate = CO2 + UMP;
CC         Xref=Rhea:RHEA:11596, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:57538, ChEBI:CHEBI:57865; EC=4.1.1.23;
CC   -!- PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway;
CC       UMP from orotate: step 2/2.
CC   -!- SIMILARITY: Belongs to the OMP decarboxylase family. Type 2 subfamily.
CC       {ECO:0000305}.
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DR   EMBL; AF187997; AAF36353.1; -; Genomic_DNA.
DR   EMBL; AE004437; AAG19923.1; -; Genomic_DNA.
DR   PIR; G84319; G84319.
DR   RefSeq; WP_010903220.1; NC_002607.1.
DR   AlphaFoldDB; Q9P9M3; -.
DR   SMR; Q9P9M3; -.
DR   STRING; 64091.VNG_1673G; -.
DR   PaxDb; 64091-VNG_1673G; -.
DR   GeneID; 68694336; -.
DR   KEGG; hal:VNG_1673G; -.
DR   PATRIC; fig|64091.14.peg.1274; -.
DR   HOGENOM; CLU_060704_1_0_2; -.
DR   InParanoid; Q9P9M3; -.
DR   OrthoDB; 94124at2157; -.
DR   PhylomeDB; Q9P9M3; -.
DR   UniPathway; UPA00070; UER00120.
DR   Proteomes; UP000000554; Chromosome.
DR   GO; GO:0004590; F:orotidine-5'-phosphate decarboxylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IEA:InterPro.
DR   GO; GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd04725; OMP_decarboxylase_like; 1.
DR   Gene3D; 3.20.20.70; Aldolase class I; 1.
DR   HAMAP; MF_01215; OMPdecase_type2; 1.
DR   InterPro; IPR013785; Aldolase_TIM.
DR   InterPro; IPR018089; OMPdecase_AS.
DR   InterPro; IPR011995; OMPdecase_type-2.
DR   InterPro; IPR001754; OMPdeCOase_dom.
DR   InterPro; IPR011060; RibuloseP-bd_barrel.
DR   NCBIfam; TIGR02127; pyrF_sub2; 1.
DR   PANTHER; PTHR43375; OROTIDINE 5'-PHOSPHATE DECARBOXYLASE; 1.
DR   PANTHER; PTHR43375:SF1; OROTIDINE 5'-PHOSPHATE DECARBOXYLASE; 1.
DR   Pfam; PF00215; OMPdecase; 1.
DR   SMART; SM00934; OMPdecase; 1.
DR   SUPFAM; SSF51366; Ribulose-phoshate binding barrel; 1.
DR   PROSITE; PS00156; OMPDECASE; 1.
PE   3: Inferred from homology;
KW   Decarboxylase; Lyase; Pyrimidine biosynthesis; Reference proteome.
FT   CHAIN           1..267
FT                   /note="Orotidine 5'-phosphate decarboxylase"
FT                   /id="PRO_0000134637"
FT   ACT_SITE        93
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   267 AA;  29048 MW;  B478E72A42572111 CRC64;
     MSFVEELGAR IEAADSVVSV GLDPDMERLP EDVQDAELPR WAFNRRIIDA THEHAAVFKP
     NAAFYEDSDG WRALRETVAY AHGKGVPVLL DAKRADIGNT ARQYAEILAH VDAITVNPYL
     GEDALQPFLT QDEAGVFVLC RTSNEGGMDF QHLELAAYDR RLYEHVAERA AEWNAQYGDV
     GLVVGATAPD ELQAIRERVP ELPFLVPGVG AQGGDAEAAV EYGLNDDGVG LVNSTRGVIF
     AGEHGSAWAA AAGDAARTLR ERLNRHR
//