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Protein

Orotidine 5'-phosphate decarboxylase

Gene

pyrF

Organism
Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Orotidine 5'-phosphate = UMP + CO2.

Pathway: UMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes UMP from orotate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Orotate phosphoribosyltransferase (pyrE)
  2. Orotidine 5'-phosphate decarboxylase (pyrF)
This subpathway is part of the pathway UMP biosynthesis via de novo pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from orotate, the pathway UMP biosynthesis via de novo pathway and in Pyrimidine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei93 – 931Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Pyrimidine biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.

Names & Taxonomyi

Protein namesi
Recommended name:
Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
Alternative name(s):
OMP decarboxylase
Short name:
OMPDCase
Short name:
OMPdecase
Gene namesi
Name:pyrF
Synonyms:ura3
Ordered Locus Names:VNG_1673G
OrganismiHalobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
Taxonomic identifieri64091 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHalobacterium
ProteomesiUP000000554 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267Orotidine 5'-phosphate decarboxylasePRO_0000134637Add
BLAST

Proteomic databases

PaxDbiQ9P9M3.

Interactioni

Protein-protein interaction databases

STRINGi64091.VNG1673G.

Structurei

3D structure databases

ProteinModelPortaliQ9P9M3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0284.
InParanoidiQ9P9M3.
KOiK01591.
OMAiCAFKPQI.
PhylomeDBiQ9P9M3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01215. OMPdecase_type2.
InterProiIPR013785. Aldolase_TIM.
IPR018089. OMPdecase_AS.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR02127. pyrF_sub2. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P9M3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVEELGAR IEAADSVVSV GLDPDMERLP EDVQDAELPR WAFNRRIIDA
60 70 80 90 100
THEHAAVFKP NAAFYEDSDG WRALRETVAY AHGKGVPVLL DAKRADIGNT
110 120 130 140 150
ARQYAEILAH VDAITVNPYL GEDALQPFLT QDEAGVFVLC RTSNEGGMDF
160 170 180 190 200
QHLELAAYDR RLYEHVAERA AEWNAQYGDV GLVVGATAPD ELQAIRERVP
210 220 230 240 250
ELPFLVPGVG AQGGDAEAAV EYGLNDDGVG LVNSTRGVIF AGEHGSAWAA
260
AAGDAARTLR ERLNRHR
Length:267
Mass (Da):29,048
Last modified:June 24, 2002 - v3
Checksum:iB478E72A42572111
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187997 Genomic DNA. Translation: AAF36353.1.
AE004437 Genomic DNA. Translation: AAG19923.1.
PIRiG84319.
RefSeqiNP_280443.1. NC_002607.1.
WP_010903220.1. NC_002607.1.

Genome annotation databases

EnsemblBacteriaiAAG19923; AAG19923; VNG_1673G.
GeneIDi1448223.
KEGGihal:VNG1673G.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF187997 Genomic DNA. Translation: AAF36353.1.
AE004437 Genomic DNA. Translation: AAG19923.1.
PIRiG84319.
RefSeqiNP_280443.1. NC_002607.1.
WP_010903220.1. NC_002607.1.

3D structure databases

ProteinModelPortaliQ9P9M3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi64091.VNG1673G.

Proteomic databases

PaxDbiQ9P9M3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG19923; AAG19923; VNG_1673G.
GeneIDi1448223.
KEGGihal:VNG1673G.

Phylogenomic databases

eggNOGiCOG0284.
InParanoidiQ9P9M3.
KOiK01591.
OMAiCAFKPQI.
PhylomeDBiQ9P9M3.

Enzyme and pathway databases

UniPathwayiUPA00070; UER00120.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01215. OMPdecase_type2.
InterProiIPR013785. Aldolase_TIM.
IPR018089. OMPdecase_AS.
IPR011995. OMPdecase_type-2.
IPR001754. OMPdeCOase_dom.
IPR011060. RibuloseP-bd_barrel.
[Graphical view]
PfamiPF00215. OMPdecase. 1 hit.
[Graphical view]
SMARTiSM00934. OMPdecase. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR02127. pyrF_sub2. 1 hit.
PROSITEiPS00156. OMPDECASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homologous gene knockout in the archaeon Halobacterium salinarum with ura3 as a counterselectable marker."
    Peck R.F., DasSarma S., Krebs M.P.
    Mol. Microbiol. 35:667-676(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: MPK1.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 700922 / JCM 11081 / NRC-1.

Entry informationi

Entry nameiPYRF_HALSA
AccessioniPrimary (citable) accession number: Q9P9M3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 23, 2002
Last sequence update: June 24, 2002
Last modified: January 7, 2015
This is version 85 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.