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Protein

Malate dehydrogenase

Gene

mdh

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible oxidation of malate to oxaloacetate.By similarity

Catalytic activityi

(S)-malate + NAD+ = oxaloacetate + NADH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei34NADBy similarity1
Binding sitei83SubstrateBy similarity1
Binding sitei89SubstrateBy similarity1
Binding sitei96NADBy similarity1
Binding sitei121SubstrateBy similarity1
Binding sitei152SubstrateBy similarity1
Active sitei176Proton acceptorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi8 – 14NADBy similarity7
Nucleotide bindingi119 – 121NADBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

NAD

Names & Taxonomyi

Protein namesi
Recommended name:
Malate dehydrogenaseBy similarity (EC:1.1.1.37By similarity)
Gene namesi
Name:mdh
Ordered Locus Names:HVO_3007
OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Taxonomic identifieri309800 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHaloferacalesHaloferacaceaeHaloferax
Proteomesi
  • UP000008243 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001134831 – 304Malate dehydrogenaseAdd BLAST304

Interactioni

Protein-protein interaction databases

STRINGi309800.HVO_3007.

Structurei

Secondary structure

1304
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 24Combined sources13
Beta strandi28 – 33Combined sources6
Helixi36 – 38Combined sources3
Helixi39 – 53Combined sources15
Turni54 – 56Combined sources3
Beta strandi60 – 63Combined sources4
Helixi66 – 69Combined sources4
Beta strandi73 – 77Combined sources5
Helixi89 – 108Combined sources20
Beta strandi115 – 118Combined sources4
Helixi123 – 132Combined sources10
Beta strandi134 – 136Combined sources3
Helixi138 – 140Combined sources3
Beta strandi141 – 143Combined sources3
Helixi146 – 161Combined sources16
Helixi165 – 167Combined sources3
Beta strandi172 – 174Combined sources3
Beta strandi180 – 182Combined sources3
Helixi184 – 186Combined sources3
Helixi198 – 216Combined sources19
Turni217 – 219Combined sources3
Helixi224 – 238Combined sources15
Beta strandi244 – 253Combined sources10
Helixi254 – 256Combined sources3
Beta strandi258 – 269Combined sources12
Beta strandi272 – 276Combined sources5
Helixi283 – 303Combined sources21

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BGUX-ray1.49A/B/C/D2-304[»]
ProteinModelPortaliQ9P9L2.
SMRiQ9P9L2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily.Curated

Phylogenomic databases

eggNOGiarCOG00246. Archaea.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00024.
OMAiDANIKGT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P9L2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTKVSVIGAA GTVGAAAGYN LALRDVCDEL VFVDIPKMED KTVGQAADTN
60 70 80 90 100
HGIAYDSNTV VTQGGYEDTA GSDVVVITAG IPRQPGQTRI DLAGDNAPIM
110 120 130 140 150
DDIGSSLAEY NDDFVSITTS NPVDLLNRHL YETGDRDRHK VIGFGGRLDS
160 170 180 190 200
ARFRYVLSQR FDVPVKNVDA TILGEHGDAQ VPVFSKVRVD GNDPAFSADE
210 220 230 240 250
KEEILGDLQE SAMDVIERKG ATQWGPATGV AHMVEAVLHD TGEVLPGSLV
260 270 280 290 300
LDGEFGYEDT AFGVPVKLGS NGIEEVVEWD LDDYEADLMD DAAEKLRDQY

DEIA
Length:304
Mass (Da):32,654
Last modified:September 21, 2011 - v2
Checksum:i7AD72EDC9C137268
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → G in AAF43044 (Ref. 1) Curated1
Sequence conflicti285Missing in AAF43044 (Ref. 1) Curated1
Sequence conflicti290D → N in AAF43044 (Ref. 1) Curated1
Sequence conflicti297R → L in AAF43044 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF236112 Genomic DNA. Translation: AAF43044.1.
DQ851095 Genomic DNA. Translation: ABH10498.1.
CP001956 Genomic DNA. Translation: ADE04975.1.
RefSeqiWP_004044925.1. NZ_AOHU01000104.1.

Genome annotation databases

EnsemblBacteriaiADE04975; ADE04975; HVO_3007.
GeneIDi8925993.
KEGGihvo:HVO_3007.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF236112 Genomic DNA. Translation: AAF43044.1.
DQ851095 Genomic DNA. Translation: ABH10498.1.
CP001956 Genomic DNA. Translation: ADE04975.1.
RefSeqiWP_004044925.1. NZ_AOHU01000104.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4BGUX-ray1.49A/B/C/D2-304[»]
ProteinModelPortaliQ9P9L2.
SMRiQ9P9L2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi309800.HVO_3007.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiADE04975; ADE04975; HVO_3007.
GeneIDi8925993.
KEGGihvo:HVO_3007.

Phylogenomic databases

eggNOGiarCOG00246. Archaea.
COG0039. LUCA.
HOGENOMiHOG000213793.
KOiK00024.
OMAiDANIKGT.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMDH_HALVD
AccessioniPrimary (citable) accession number: Q9P9L2
Secondary accession number(s): D4GYB6, Q0MSE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: September 21, 2011
Last modified: November 30, 2016
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.