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Q9P9L2 (MDH_HALVD) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Malate dehydrogenase

EC=1.1.1.37
Gene names
Name:mdh
Ordered Locus Names:HVO_3007
OrganismHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii) [Reference proteome] [HAMAP]
Taxonomic identifier309800 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the reversible oxidation of malate to oxaloacetate By similarity. HAMAP-Rule MF_00487

Catalytic activity

(S)-malate + NAD+ = oxaloacetate + NADH. HAMAP-Rule MF_00487

Sequence similarities

Belongs to the LDH/MDH superfamily. MDH type 3 family.

Ontologies

Keywords
   Biological processTricarboxylic acid cycle
   LigandNAD
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcellular carbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

malate metabolic process

Inferred from electronic annotation. Source: InterPro

tricarboxylic acid cycle

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Molecular_functionL-malate dehydrogenase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Malate dehydrogenase HAMAP-Rule MF_00487
PRO_0000113483

Regions

Nucleotide binding8 – 147NAD By similarity
Nucleotide binding119 – 1213NAD By similarity

Sites

Active site1761Proton acceptor By similarity
Binding site341NAD By similarity
Binding site831Substrate By similarity
Binding site891Substrate By similarity
Binding site961NAD By similarity
Binding site1211Substrate By similarity
Binding site1521Substrate By similarity

Experimental info

Sequence conflict171A → G in AAF43044. Ref.1
Sequence conflict2851Missing in AAF43044. Ref.1
Sequence conflict2901D → N in AAF43044. Ref.1
Sequence conflict2971R → L in AAF43044. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9P9L2 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: 7AD72EDC9C137268

FASTA30432,654
        10         20         30         40         50         60 
MTKVSVIGAA GTVGAAAGYN LALRDVCDEL VFVDIPKMED KTVGQAADTN HGIAYDSNTV 

        70         80         90        100        110        120 
VTQGGYEDTA GSDVVVITAG IPRQPGQTRI DLAGDNAPIM DDIGSSLAEY NDDFVSITTS 

       130        140        150        160        170        180 
NPVDLLNRHL YETGDRDRHK VIGFGGRLDS ARFRYVLSQR FDVPVKNVDA TILGEHGDAQ 

       190        200        210        220        230        240 
VPVFSKVRVD GNDPAFSADE KEEILGDLQE SAMDVIERKG ATQWGPATGV AHMVEAVLHD 

       250        260        270        280        290        300 
TGEVLPGSLV LDGEFGYEDT AFGVPVKLGS NGIEEVVEWD LDDYEADLMD DAAEKLRDQY 


DEIA 

« Hide

References

« Hide 'large scale' references
[1]"The gene coding for Haloferax volcanii malate dehydrogenase."
Mevarech M.
Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Expression of halophilic malate dehydrogenase of Haloferax volcanii."
Tetsch L., Galinski E.A.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
[3]"The complete genome sequence of Haloferax volcanii DS2, a model archaeon."
Hartman A.L., Norais C., Badger J.H., Delmas S., Haldenby S., Madupu R., Robinson J., Khouri H., Ren Q., Lowe T.M., Maupin-Furlow J., Pohlschroder M., Daniels C., Pfeiffer F., Allers T., Eisen J.A.
PLoS ONE 5:E9605-E9605(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF236112 Genomic DNA. Translation: AAF43044.1.
DQ851095 Genomic DNA. Translation: ABH10498.1.
CP001956 Genomic DNA. Translation: ADE04975.1.
RefSeqYP_003537012.1. NC_013967.1.

3D structure databases

ProteinModelPortalQ9P9L2.
SMRQ9P9L2. Positions 2-304.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaADE04975; ADE04975; HVO_3007.
GeneID8925993.
KEGGhvo:HVO_3007.

Phylogenomic databases

HOGENOMHOG000213793.
KOK00024.
OMADYLEDTI.

Enzyme and pathway databases

BioCycHVOL309800:GCOK-3013-MONOMER.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPMF_00487. Malate_dehydrog_3.
InterProIPR001557. L-lactate/malate_DH.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR011275. Malate_DH_type3.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11540. PTHR11540. 1 hit.
PfamPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSPR00086. LLDHDRGNASE.
SUPFAMSSF56327. SSF56327. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMDH_HALVD
AccessionPrimary (citable) accession number: Q9P9L2
Secondary accession number(s): D4GYB6, Q0MSE3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: September 21, 2011
Last modified: February 19, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families