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Q9P9L2

- MDH_HALVD

UniProt

Q9P9L2 - MDH_HALVD

Protein

Malate dehydrogenase

Gene

mdh

Organism
Haloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 76 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible oxidation of malate to oxaloacetate.UniRule annotation

    Catalytic activityi

    (S)-malate + NAD+ = oxaloacetate + NADH.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei34 – 341NADUniRule annotation
    Binding sitei83 – 831SubstrateUniRule annotation
    Binding sitei89 – 891SubstrateUniRule annotation
    Binding sitei96 – 961NADUniRule annotation
    Binding sitei121 – 1211SubstrateUniRule annotation
    Binding sitei152 – 1521SubstrateUniRule annotation
    Active sitei176 – 1761Proton acceptorUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 147NADUniRule annotation
    Nucleotide bindingi119 – 1213NADUniRule annotation

    GO - Molecular functioni

    1. L-malate dehydrogenase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. cellular carbohydrate metabolic process Source: InterPro
    2. malate metabolic process Source: InterPro
    3. tricarboxylic acid cycle Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Tricarboxylic acid cycle

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    BioCyciHVOL309800:GCOK-3013-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate dehydrogenaseUniRule annotation (EC:1.1.1.37UniRule annotation)
    Gene namesi
    Name:mdhUniRule annotation
    Ordered Locus Names:HVO_3007
    OrganismiHaloferax volcanii (strain ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2) (Halobacterium volcanii)
    Taxonomic identifieri309800 [NCBI]
    Taxonomic lineageiArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeHaloferax
    ProteomesiUP000008243: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 304304Malate dehydrogenasePRO_0000113483Add
    BLAST

    Structurei

    Secondary structure

    1
    304
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi3 – 86
    Helixi12 – 2413
    Beta strandi28 – 336
    Helixi36 – 383
    Helixi39 – 5315
    Turni54 – 563
    Beta strandi60 – 634
    Helixi66 – 694
    Beta strandi73 – 775
    Helixi89 – 10820
    Beta strandi115 – 1184
    Helixi123 – 13210
    Beta strandi134 – 1363
    Helixi138 – 1403
    Beta strandi141 – 1433
    Helixi146 – 16116
    Helixi165 – 1673
    Beta strandi172 – 1743
    Beta strandi180 – 1823
    Helixi184 – 1863
    Helixi198 – 21619
    Turni217 – 2193
    Helixi224 – 23815
    Beta strandi244 – 25310
    Helixi254 – 2563
    Beta strandi258 – 26912
    Beta strandi272 – 2765
    Helixi283 – 30321

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    4BGUX-ray1.49A/B/C/D2-304[»]
    ProteinModelPortaliQ9P9L2.
    SMRiQ9P9L2. Positions 2-304.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the LDH/MDH superfamily. MDH type 3 family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000213793.
    KOiK00024.
    OMAiLMSAGMP.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPiMF_00487. Malate_dehydrog_3.
    InterProiIPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR11540. PTHR11540. 1 hit.
    PfamiPF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSiPR00086. LLDHDRGNASE.
    SUPFAMiSSF56327. SSF56327. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9P9L2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTKVSVIGAA GTVGAAAGYN LALRDVCDEL VFVDIPKMED KTVGQAADTN    50
    HGIAYDSNTV VTQGGYEDTA GSDVVVITAG IPRQPGQTRI DLAGDNAPIM 100
    DDIGSSLAEY NDDFVSITTS NPVDLLNRHL YETGDRDRHK VIGFGGRLDS 150
    ARFRYVLSQR FDVPVKNVDA TILGEHGDAQ VPVFSKVRVD GNDPAFSADE 200
    KEEILGDLQE SAMDVIERKG ATQWGPATGV AHMVEAVLHD TGEVLPGSLV 250
    LDGEFGYEDT AFGVPVKLGS NGIEEVVEWD LDDYEADLMD DAAEKLRDQY 300
    DEIA 304
    Length:304
    Mass (Da):32,654
    Last modified:September 21, 2011 - v2
    Checksum:i7AD72EDC9C137268
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti17 – 171A → G in AAF43044. 1 PublicationCurated
    Sequence conflicti285 – 2851Missing in AAF43044. 1 PublicationCurated
    Sequence conflicti290 – 2901D → N in AAF43044. 1 PublicationCurated
    Sequence conflicti297 – 2971R → L in AAF43044. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF236112 Genomic DNA. Translation: AAF43044.1.
    DQ851095 Genomic DNA. Translation: ABH10498.1.
    CP001956 Genomic DNA. Translation: ADE04975.1.
    RefSeqiYP_003537012.1. NC_013967.1.

    Genome annotation databases

    EnsemblBacteriaiADE04975; ADE04975; HVO_3007.
    GeneIDi8925993.
    KEGGihvo:HVO_3007.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF236112 Genomic DNA. Translation: AAF43044.1 .
    DQ851095 Genomic DNA. Translation: ABH10498.1 .
    CP001956 Genomic DNA. Translation: ADE04975.1 .
    RefSeqi YP_003537012.1. NC_013967.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    4BGU X-ray 1.49 A/B/C/D 2-304 [» ]
    ProteinModelPortali Q9P9L2.
    SMRi Q9P9L2. Positions 2-304.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ADE04975 ; ADE04975 ; HVO_3007 .
    GeneIDi 8925993.
    KEGGi hvo:HVO_3007.

    Phylogenomic databases

    HOGENOMi HOG000213793.
    KOi K00024.
    OMAi LMSAGMP.

    Enzyme and pathway databases

    BioCyci HVOL309800:GCOK-3013-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    3.90.110.10. 1 hit.
    HAMAPi MF_00487. Malate_dehydrog_3.
    InterProi IPR001557. L-lactate/malate_DH.
    IPR022383. Lactate/malate_DH_C.
    IPR001236. Lactate/malate_DH_N.
    IPR015955. Lactate_DH/Glyco_Ohase_4_C.
    IPR011275. Malate_DH_type3.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR11540. PTHR11540. 1 hit.
    Pfami PF02866. Ldh_1_C. 1 hit.
    PF00056. Ldh_1_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000102. Lac_mal_DH. 1 hit.
    PRINTSi PR00086. LLDHDRGNASE.
    SUPFAMi SSF56327. SSF56327. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The gene coding for Haloferax volcanii malate dehydrogenase."
      Mevarech M.
      Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Expression of halophilic malate dehydrogenase of Haloferax volcanii."
      Tetsch L., Galinski E.A.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 29605 / DSM 3757 / JCM 8879 / NBRC 14742 / NCIMB 2012 / VKM B-1768 / DS2.

    Entry informationi

    Entry nameiMDH_HALVD
    AccessioniPrimary (citable) accession number: Q9P9L2
    Secondary accession number(s): D4GYB6, Q0MSE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 76 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3