Reviewed, UniProtKB/Swiss-Prot Q9P9K9 (DNLI_SULSH)

Names and origin

Protein names

Recommended name:
    DNA ligase
    EC=6.5.1.1
Alternative name(s):
    Polydeoxyribonucleotide synthase [ATP]

Gene names

Name:

lig

Organism

Sulfolobus shibatae

Taxonomic identifier

2286 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	601 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair By similarity.
Catalytic activity	ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m). HAMAP MF_00407
Cofactor	Magnesium By similarity.
Sequence similarities	Belongs to the ATP-dependent DNA ligase family.

Ontologies

Keywords
Biological process	Cell cycle Cell division DNA damage DNA recombination DNA repair DNA replication
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: InterPro DNA ligase (ATP) activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 601	601	DNA ligase HAMAP MF_00407		PRO_0000059617
Sites
	Active site	260	1	N6-AMP-lysine intermediate By similarity
	Binding site	258	1	ATP By similarity
	Binding site	265	1	ATP By similarity
	Binding site	280	1	ATP By similarity
	Binding site	310	1	ATP By similarity
	Binding site	350	1	ATP By similarity
	Binding site	427	1	ATP By similarity
	Binding site	433	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P9K9-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: C029AC84AB39AFD4	FASTA	601	67,647
	10 20 30 40 50 60 MEFKVIAEYF DKLEKISSRL QLTALLADLL SKSDKAIIDK VVYIIQGKLW PDFLGYPELG 70 80 90 100 110 120 IGEKFLIKAI SIATNTDENS VENLYKSTGD LGEVARRLKS KQQSTGILGF LGTSSKESLT 130 140 150 160 170 180 VDEVYSTLSK VALTTGEGSR DLKIRLLAGL LKKADPLEAK FLVRFVEGRL RVGIGDATVL 190 200 210 220 230 240 DAMAIAFGGG QSASEIVERA YNLRADLGNI AKIIVEKGIE ALKTLKPEVG IPIRPMLAER 250 260 270 280 290 300 LSNPEEILKK VGGSALVDYK YDGERAQIHK KSDKIFIFSR RLENITSQYP DVVEYISKYV 310 320 330 340 350 360 EGKEFIIEGE IVAVDPESGE MRAFQELMHR KRKSDIYEAI KEYPVNVFLF DLMYYEDVDY 370 380 390 400 410 420 TTKPLEVRRK LLESIVKPND YVKIAHHIQV NNVEDLKSFF YRAISEGGEG VMVKAIGKDA 430 440 450 460 470 480 IYQAGARGWL WIKLKRDYQS EMADTVDLVV VGGFYGKGKR GGKISSLLMA AYNPKTDTFE 490 500 510 520 530 540 SVCKVASGFS DEQLDELQKK LMEIKRDIKH PRVNSKMEPD IWIEPVYVAE IIGAEITISP 550 560 570 580 590 600 LHTCCQDVVE KDAGLSIRFP RFIRWRDDKS PEDATTTDEI LEMYNKQPKK KIESPLVDES V

References

[1]	"A thermophilic DNA ligase from Sulfolobus shibatae." Lai X., Shao H., Huang L. Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF242877 Genomic DNA. Translation: AAF61267.1.
3D structure databases
SMR	Q9P9K9. Positions 1-590.
ModBase	Search...
Enzyme and pathway databases
BRENDA	6.5.1.1. 2332.
Family and domain databases
HAMAP	MF_00407. [Tree]
InterPro	IPR000977. DNA_ligase. IPR012309. DNA_ligase_A_C. IPR012310. DNA_ligase_A_M. IPR012308. DNA_ligase_A_N. IPR016059. DNA_ligase_CS. IPR012340. NA-bd_OB-fold. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
Pfam	PF04679. DNA_ligase_A_C. 1 hit. PF01068. DNA_ligase_A_M. 1 hit. PF04675. DNA_ligase_A_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00574. dnl1. 1 hit.
PROSITE	PS00697. DNA_LIGASE_A1. 1 hit. PS00333. DNA_LIGASE_A2. 1 hit. PS50160. DNA_LIGASE_A3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNLI_SULSH

Accession

Primary (citable) accession number: Q9P9K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	March 24, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)