Reviewed, UniProtKB/Swiss-Prot Q9P999 (XYLS_SULSO)

Names and origin

Protein names

Recommended name:
    Alpha-xylosidase
    EC=3.2.1.-

Gene names

Name:	xylS
Ordered Locus Names:	SSO3022

Organism

Sulfolobus solfataricus [Complete proteome] [HAMAP]

Taxonomic identifier

2287 [NCBI]

Taxonomic lineage

Archaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the liberation of alpha-xylose from the non-reducing terminal glucose of xyloglucan oligosaccharides. Has high hydrolytic activity on the disaccharide isoprimeverose. Follows a retaining mechanism of substrate hydrolysis.
Subunit structure	Monomer.
Sequence similarities	Belongs to the glycosyl hydrolase 31 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	Alpha-xylosidase		PRO_0000185369
Sites
	Active site	353	1	By similarity
	Active site	356	1	By similarity
	Active site	428	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P999-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 85328243A00C4F3E	FASTA	731	84,485
	10 20 30 40 50 60 MRIGNLNVEI EFIADNIVRV LYYYGREAIV DNSLVVLPNL EKLSIKGEST GPSIISFSSE 70 80 90 100 110 120 SLSVDINTSN GELIMKDNKG GIVVKEKRRD LKFNEELSTY NVEQEFELSE GERVYGLGQH 130 140 150 160 170 180 AGGNGLGQSS AYKLDYSGLS TTLSQRNTDI GIPFIVSSKG YGILWDNYSL GSISLRRNKL 190 200 210 220 230 240 KVWFEAGKKI DYYVIYGDSI DDVIKGYRKL TGDAPLLPKW AYGYWQSKER YKSQDEITSV 250 260 270 280 290 300 VKEFRERKIP LDVIVLDWRY WGKYGWNAFK FDETDFPRPK DMVEEIHKMG AKLAISIWPT 310 320 330 340 350 360 FGKETEVFKD MESKGCIILG TTAFNPFKDE CRELFWSYVK GFYDLGIDAY WLDASEPETG 370 380 390 400 410 420 LGLVFFSPIH DVDLEIGKGY EYLNAYPLME TKAVYEGQRR ISNKRVVILT RSAFAGQQRH 430 440 450 460 470 480 SAISWSGDVL GDWATLRAQI PAGLNFSISG IPYWTTDTGG FFSGNPETKA YAEIFVRWFQ 490 500 510 520 530 540 WSTFCPILRV HGTIFPKEPW RFPREYQEVI LKYIRLRYKL LPYIYSLAWM TYSIGYTIMR 550 560 570 580 590 600 PLVMDFRDDQ NVYDFDEQYM FGPYILISPV TLPSIIEKEV YLPSKEYWYD FWTGEKLEGG 610 620 630 640 650 660 RMMDVKVTLD TIPLFVRSGA VLPLLGKNVN NAEEYWDVID LRVYPGKNGY FELYDDDGIT 670 680 690 700 710 720 YEYEKGKYYI IPITWDEDKQ ELTIGKKRGE LEMSKKIIKI IWVEKGKGIE HTKPDVEIEY 730 NGKETITVKR G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and molecular characterization of the first alpha-xylosidase from an Archaeon." Moracci M., Ponzano B.C., Trincone A., Fusco S., De Rosa M., van der Oost J., Sensen C.W., Charlebois R.L., Rossi M. J. Biol. Chem. 275:22082-22089(2000) [PubMed: 10801892] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2 and DSM 5833 / MT-4.
[2]	"The complete genome of the crenarchaeon Sulfolobus solfataricus P2." She Q., Singh R.K., Confalonieri F., Zivanovic Y., Allard G., Awayez M.J., Chan-Weiher C.C.-Y., Clausen I.G., Curtis B.A., De Moors A., Erauso G., Fletcher C., Gordon P.M.K., Heikamp-de Jong I., Jeffries A.C., Kozera C.J., Medina N., Peng X. , Thi-Ngoc H.P., Redder P., Schenk M.E., Theriault C., Tolstrup N., Charlebois R.L., Doolittle W.F., Duguet M., Gaasterland T., Garrett R.A., Ragan M.A., Sensen C.W., Van der Oost J. Proc. Natl. Acad. Sci. U.S.A. 98:7835-7840(2001) [PubMed: 11427726] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 35092 / DSM 1617 / JCM 11322 / P2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ251975 Genomic DNA. Translation: CAB99206.1. AE006641 Genomic DNA. Translation: AAK43123.1.
PIR	D90483.
RefSeq	NP_344333.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH31. Glycoside Hydrolase Family 31.
Genome annotation databases
GeneID	1453042.
GenomeReviews	Gene locus SSO3022 in contig AE006641_GR.
KEGG	sso:SSO3022.
NMPDR	fig|273057.1.peg.2756.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9P999.
OMA	Q9P999. MSGADLP.
Enzyme and pathway databases
BioCyc	SSOL273057:SSO3022-MON.
Family and domain databases
InterPro	IPR000322. Glyco_hydro_31. [Graphical view]
PANTHER	PTHR22762. Glyco_hydro_31. 1 hit.
Pfam	PF01055. Glyco_hydro_31. 1 hit. [Graphical view]
PROSITE	PS00129. GLYCOSYL_HYDROL_F31_1. False negative. PS00707. GLYCOSYL_HYDROL_F31_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

XYLS_SULSO

Accession

Primary (citable) accession number: Q9P999

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)