Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9P999 (XYLS_SULSO) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 56. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alpha-xylosidase
EC=3.2.1.177
Gene names
Name:xylS
Ordered Locus Names:SSO3022
OrganismSulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
Taxonomic identifier273057 [NCBI]
Taxonomic lineageArchaea › Crenarchaeota › Thermoprotei › Sulfolobales › Sulfolobaceae › Sulfolobus

Protein attributes

Sequence length731 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the liberation of alpha-xylose from the non-reducing terminal glucose of xyloglucan oligosaccharides. Has high hydrolytic activity on the disaccharide isoprimeverose. Follows a retaining mechanism of substrate hydrolysis.

Catalytic activity

Hydrolysis of terminal, non-reducing alpha-D-xylose residues with release of alpha-D-xylose. Ref.1

Subunit structure

Monomer. Ref.1

Sequence similarities

Belongs to the glycosyl hydrolase 31 family.

Biophysicochemical properties

Kinetic parameters:

KM=17 mM for maltose Ref.1

KM=3.45 mM for maltotriose

KM=28.9 mM for isoprimeverose

KM=1.72 mM for 4-nitrophenyl-beta-isoprimeveroside

pH dependence:

Optimum pH is 5.5.

Temperature dependence:

Optimum temperature is 90 degrees Celsius.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 731731Alpha-xylosidase
PRO_0000185369

Sites

Active site3531 By similarity
Active site3561 By similarity
Active site4281Proton donor By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9P999 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: 85328243A00C4F3E

FASTA73184,485
        10         20         30         40         50         60 
MRIGNLNVEI EFIADNIVRV LYYYGREAIV DNSLVVLPNL EKLSIKGEST GPSIISFSSE 

        70         80         90        100        110        120 
SLSVDINTSN GELIMKDNKG GIVVKEKRRD LKFNEELSTY NVEQEFELSE GERVYGLGQH 

       130        140        150        160        170        180 
AGGNGLGQSS AYKLDYSGLS TTLSQRNTDI GIPFIVSSKG YGILWDNYSL GSISLRRNKL 

       190        200        210        220        230        240 
KVWFEAGKKI DYYVIYGDSI DDVIKGYRKL TGDAPLLPKW AYGYWQSKER YKSQDEITSV 

       250        260        270        280        290        300 
VKEFRERKIP LDVIVLDWRY WGKYGWNAFK FDETDFPRPK DMVEEIHKMG AKLAISIWPT 

       310        320        330        340        350        360 
FGKETEVFKD MESKGCIILG TTAFNPFKDE CRELFWSYVK GFYDLGIDAY WLDASEPETG 

       370        380        390        400        410        420 
LGLVFFSPIH DVDLEIGKGY EYLNAYPLME TKAVYEGQRR ISNKRVVILT RSAFAGQQRH 

       430        440        450        460        470        480 
SAISWSGDVL GDWATLRAQI PAGLNFSISG IPYWTTDTGG FFSGNPETKA YAEIFVRWFQ 

       490        500        510        520        530        540 
WSTFCPILRV HGTIFPKEPW RFPREYQEVI LKYIRLRYKL LPYIYSLAWM TYSIGYTIMR 

       550        560        570        580        590        600 
PLVMDFRDDQ NVYDFDEQYM FGPYILISPV TLPSIIEKEV YLPSKEYWYD FWTGEKLEGG 

       610        620        630        640        650        660 
RMMDVKVTLD TIPLFVRSGA VLPLLGKNVN NAEEYWDVID LRVYPGKNGY FELYDDDGIT 

       670        680        690        700        710        720 
YEYEKGKYYI IPITWDEDKQ ELTIGKKRGE LEMSKKIIKI IWVEKGKGIE HTKPDVEIEY 

       730 
NGKETITVKR G

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ251975 Genomic DNA. Translation: CAB99206.1.
AE006641 Genomic DNA. Translation: AAK43123.1.
PIRD90483.
RefSeqNP_344333.1. NC_002754.1.

3D structure databases

ProteinModelPortalQ9P999.
ModBaseSearch...

Protein family/group databases

CAZyGH31. Glycoside Hydrolase Family 31.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1453042.
GenomeReviewsGene locus SSO3022 in contig AE006641_GR.
KEGGsso:SSO3022.
NMPDRfig|273057.1.peg.2756.

Phylogenomic databases

HOGENOMHBG346343.
OMAGIPFMIS.
ProtClustDBCLSK2484581.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-16613.
SSOL273057:SSO3022-MONOMER.

Family and domain databases

InterProIPR011013. Glyco_hydro-type_carb-bd.
IPR000322. Glyco_hydro_31.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
KOK01811.
PANTHERPTHR22762. Glyco_hydro_31. 1 hit.
PfamPF01055. Glyco_hydro_31. 1 hit.
[Graphical view]
SUPFAMSSF74650. Gal_mut_like. 1 hit.
SSF51445. Glyco_hydro_cat. 1 hit.
PROSITEPS00129. GLYCOSYL_HYDROL_F31_1. False negative.
PS00707. GLYCOSYL_HYDROL_F31_2. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameXYLS_SULSO
AccessionPrimary (citable) accession number: Q9P999
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2001
Last sequence update: October 1, 2000
Last modified: January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents