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Protein

Proteasome subunit beta

Gene

psmB

Organism
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.UniRule annotation

Catalytic activityi

Cleavage of peptide bonds with very broad specificity.UniRule annotation

Enzyme regulationi

The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei12NucleophileUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Threonine protease

Protein family/group databases

MEROPSiT01.002.

Names & Taxonomyi

Protein namesi
Recommended name:
Proteasome subunit betaUniRule annotation (EC:3.4.25.1UniRule annotation)
Alternative name(s):
20S proteasome beta subunitUniRule annotation
Proteasome core protein PsmBUniRule annotation
Gene namesi
Name:psmBUniRule annotation
Ordered Locus Names:AF_0481
OrganismiArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
Taxonomic identifieri224325 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaArchaeoglobiArchaeoglobalesArchaeoglobaceaeArchaeoglobus
Proteomesi
  • UP000002199 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Proteasome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PropeptideiPRO_00000266591 – 11Removed in mature form; by autocatalysisUniRule annotationAdd BLAST11
ChainiPRO_000002666012 – 213Proteasome subunit betaAdd BLAST202

Keywords - PTMi

Autocatalytic cleavage, Zymogen

Interactioni

Subunit structurei

The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has probably a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is likely capped at one or both ends by the proteasome regulatory ATPase, PAN.1 Publication

Protein-protein interaction databases

IntActiQ9P996. 1 interactor.
STRINGi224325.AF0481.

Structurei

Secondary structure

1213
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi14 – 19Combined sources6
Beta strandi22 – 28Combined sources7
Beta strandi31 – 33Combined sources3
Beta strandi36 – 41Combined sources6
Beta strandi45 – 49Combined sources5
Beta strandi52 – 58Combined sources7
Helixi60 – 81Combined sources22
Helixi87 – 100Combined sources14
Turni101 – 103Combined sources3
Beta strandi108 – 116Combined sources9
Beta strandi119 – 125Combined sources7
Beta strandi131 – 141Combined sources11
Helixi144 – 154Combined sources11
Helixi161 – 176Combined sources16
Beta strandi186 – 191Combined sources6
Beta strandi196 – 198Combined sources3
Helixi200 – 207Combined sources8
Helixi208 – 210Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2QX-ray2.83H/I/J/K/L/M/N12-213[»]
ProteinModelPortaliQ9P996.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9P996.

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase T1B family.UniRule annotation

Phylogenomic databases

eggNOGiarCOG00970. Archaea.
COG0638. LUCA.
KOiK03433.
OMAiMVDWLGT.

Family and domain databases

CDDicd03764. proteasome_beta_archeal. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_A. Proteasome_B_A. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P996-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSMIEEKIYK GTTTVGLVCK DGVVMATEKR ATMGNFIASK AAKKIYQIAD
60 70 80 90 100
RMAMTTAGSV GDAQFLARII KIEANLYEIR RERKPTVRAI ATLTSNLLNS
110 120 130 140 150
YRYFPYLVQL LIGGIDSEGK SIYSIDPIGG AIEEKDIVAT GSGSLTAYGV
160 170 180 190 200
LEDRFTPEIG VDEAVELAVR AIYSAMKRDS ASGDGIDVVK ITEDEFYQYS
210
PEEVEQILAK FRK
Length:213
Mass (Da):23,418
Last modified:October 1, 2000 - v1
Checksum:iDA03E480A88F8227
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90757.1.
PIRiA69310.

Genome annotation databases

EnsemblBacteriaiAAB90757; AAB90757; AF_0481.
KEGGiafu:AF_0481.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE000782 Genomic DNA. Translation: AAB90757.1.
PIRiA69310.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J2QX-ray2.83H/I/J/K/L/M/N12-213[»]
ProteinModelPortaliQ9P996.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9P996. 1 interactor.
STRINGi224325.AF0481.

Protein family/group databases

MEROPSiT01.002.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAB90757; AAB90757; AF_0481.
KEGGiafu:AF_0481.

Phylogenomic databases

eggNOGiarCOG00970. Archaea.
COG0638. LUCA.
KOiK03433.
OMAiMVDWLGT.

Miscellaneous databases

EvolutionaryTraceiQ9P996.

Family and domain databases

CDDicd03764. proteasome_beta_archeal. 1 hit.
Gene3Di3.60.20.10. 1 hit.
HAMAPiMF_02113_A. Proteasome_B_A. 1 hit.
InterProiIPR029055. Ntn_hydrolases_N.
IPR019983. Pept_T1A_Psome_bsu_arc.
IPR000243. Pept_T1A_subB.
IPR016050. Proteasome_bsu_CS.
IPR001353. Proteasome_sua/b.
IPR023333. Proteasome_suB-type.
[Graphical view]
PfamiPF00227. Proteasome. 1 hit.
[Graphical view]
PRINTSiPR00141. PROTEASOME.
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR03634. arc_protsome_B. 1 hit.
PROSITEiPS00854. PROTEASOME_BETA_1. 1 hit.
PS51476. PROTEASOME_BETA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSB_ARCFU
AccessioniPrimary (citable) accession number: Q9P996
Secondary accession number(s): O29769
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 29, 2001
Last sequence update: October 1, 2000
Last modified: November 30, 2016
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.