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Protein

Isocitrate lyase

Gene

ICL1

Organism
Candida albicans (Yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of succinate and glyoxylate from isocitrate, a key step of the glyoxylate cycle, which operates as an anaplerotic route for replenishing the tricarboxylic acid cycle. Required for growth on ethanol or acetate, but dispensable when fermentable carbon sources are available. Acts also on 2-methylisocitrate.By similarity

Catalytic activityi

Isocitrate = succinate + glyoxylate.By similarity
(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate.By similarity

Cofactori

Mg2+By similarity

Pathwayi: glyoxylate cycle

This protein is involved in step 1 of the subpathway that synthesizes (S)-malate from isocitrate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (ICL1)
  2. Malate synthase (MLS1)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi172MagnesiumBy similarity1
Active sitei210Proton acceptorBy similarity1
Binding sitei247SubstrateBy similarity1
Binding sitei464SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BRENDAi4.1.3.1. 1096.
UniPathwayiUPA00703; UER00719.

Names & Taxonomyi

Protein namesi
Recommended name:
Isocitrate lyase1 Publication (EC:4.1.3.1By similarity)
Short name:
ICLCurated
Short name:
IsocitraseCurated
Short name:
IsocitrataseCurated
Alternative name(s):
Methylisocitrate lyaseBy similarity (EC:4.1.3.30By similarity)
Short name:
MICACurated
Threo-D(S)-isocitrate glyoxylate-lyaseCurated
Gene namesi
Name:ICL11 Publication
OrganismiCandida albicans (Yeast)
Taxonomic identifieri5476 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesDebaryomycetaceaeCandida/Lodderomyces cladeCandida

Subcellular locationi

  • Glyoxysome By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Glyoxysome, Peroxisome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5359.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000687841 – 550Isocitrate lyaseAdd BLAST550

Interactioni

Subunit structurei

Homotetramer.By similarity

Chemistry databases

BindingDBiQ9P8Q7.

Structurei

3D structure databases

ProteinModelPortaliQ9P8Q7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni101 – 103Substrate bindingBy similarity3
Regioni211 – 212Substrate bindingBy similarity2
Regioni430 – 434Substrate bindingBy similarity5

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi548 – 550Microbody targeting signalSequence analysis3

Sequence similaritiesi

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P8Q7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYTPIDIQK EEADFQKEVA EIKKWWSEPR WRKTKRIYSA EDIAKKRGTL
60 70 80 90 100
KINHPSSQQA DKLFKLLETH DADKTVSFTF GALDPIHVAQ MAKYLDSIYV
110 120 130 140 150
SGWQCSSTAS TSNEPSPDLA DYPMDTVPNK VEHLWFAQLF HDRKQREERL
160 170 180 190 200
TLSKEERAKT PYIDFLRPII ADADTGHGGI TAIIKLTKMF IERGAAGIHI
210 220 230 240 250
EDQAPGTKKC GHMAGKVLVP VQEHINRLVA IRASADIFGS NLLAVARTDS
260 270 280 290 300
EAATLITSTI DHRDHYFIIG ATNPEAGDLA ALMAEAESKG IYGNELAAIE
310 320 330 340 350
SEWTKKAGLK LFHEAVIDEI KNGNYSNKDA LIKKFTDKVN PLSHTSHKEA
360 370 380 390 400
KKLAKELTGK DIYFNWDVAR AREGYYRYQG GTQCAVMRGR AFAPYADLIW
410 420 430 440 450
MESALPDYAQ AKEFADGVKA AVPDQWLAYN LSPSFNWNKA MPADEQETYI
460 470 480 490 500
KRLGKLGYVW QFITLAGLHT TALAVDDFSN QYSQIGMKAY GQTVQQPEIE
510 520 530 540 550
KGVEVVKHQK WSGATYIDGL LKMVSGGVTS TAAMGQGVTE DQFKESKAKA
Length:550
Mass (Da):61,395
Last modified:October 1, 2000 - v1
Checksum:iFFBDB59FB60BE337
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222905 Genomic DNA. Translation: AAF34690.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF222905 Genomic DNA. Translation: AAF34690.1.

3D structure databases

ProteinModelPortaliQ9P8Q7.
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiQ9P8Q7.
ChEMBLiCHEMBL5359.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00703; UER00719.
BRENDAi4.1.3.1. 1096.

Family and domain databases

Gene3Di3.20.20.60. 2 hits.
InterProiIPR006254. Isocitrate_lyase.
IPR018523. Isocitrate_lyase_ph_CS.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
[Graphical view]
PfamiPF00463. ICL. 1 hit.
[Graphical view]
SUPFAMiSSF51621. SSF51621. 1 hit.
TIGRFAMsiTIGR01346. isocit_lyase. 1 hit.
PROSITEiPS00161. ISOCITRATE_LYASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiACEA_CANAX
AccessioniPrimary (citable) accession number: Q9P8Q7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: October 1, 2000
Last modified: October 5, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.