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Q9P8P2

- PLB1_CRYNH

UniProt

Q9P8P2 - PLB1_CRYNH

Protein

Phospholipase B

Gene

PLB1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 2 (06 Feb 2013)
      Previous versions | rss
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    Functioni

    Exhibits phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities.

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Inhibited by Fe3+ ion.

    pH dependencei

    Active only at acidic pHs.

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC

    GO - Biological processi

    1. phospholipid catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase B (EC:3.1.1.5)
    Alternative name(s):
    Lysophospholipase
    Gene namesi
    Name:PLB1
    ORF Names:CNAG_06085
    OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
    Taxonomic identifieri235443 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000010091: Chromosome 12

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1919Sequence AnalysisAdd
    BLAST
    Chaini20 – 637618Phospholipase BPRO_0000024636Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi553 – 5531N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi572 – 5721N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi594 – 5941N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi606 – 6061N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.

    Keywords - PTMi

    Glycoprotein

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 572527PLA2cPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the lysophospholipase family.Curated
    Contains 1 PLA2c domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    OrthoDBiEOG7N37NC.

    Family and domain databases

    InterProiIPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view]
    PfamiPF01735. PLA2_B. 1 hit.
    [Graphical view]
    SMARTiSM00022. PLAc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52151. SSF52151. 1 hit.
    PROSITEiPS51210. PLA2C. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9P8P2-1 [UniParc]FASTAAdd to Basket

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    MSIATATFAF SLFATIAFAV PPETPRIELQ AERGLGDKSY APWQVDCPSN    50
    VTWIRNATTG LGSGERAYIE AREKLVQPVI EQMMAARGLE TPPRTPNIGV 100
    ALSGGGYRAM LTGLGGIMGM MNESTEASES ETGGWLDGVS YWAGLSGGSW 150
    ATGTFMSNGG QLPTNLLENL WNIDSNLVFP DDDKLSFYTE LYTETNAKSD 200
    LGFPIQITDV WGLAIGSHVL PERYQLSNTP NLTFSSLPSV VSALGNASLP 250
    MPIIIAADRK RREAGELVIA ENATVWEFTP YEFGSWAFGS QYKSPGAFTP 300
    IEYLGTSVDD GSPNGTCWKG FDQLSFVMGT SATLFNGAFL ELNGTDSGLL 350
    TNLITAFLAD LGEDQADISR IPNTFSNYNS GENPIYNLTY ITLVDAGETN 400
    QNIPLEPLLV PTRDVDAIVA FDSSYDTDYI WPNGTALRTT YERAKVLAEH 450
    ENTRVLMPEV PSMNGFVNGG YNSRPTFFGC NDTTTPLIIY VPSYPWSFAA 500
    NTSTYQLSYE NDEANEMLLN GMRSLTLNHS VPTWPTCFAC ALTDRSFMYT 550
    SENRSTTCQK CFDTWCWAGD DNTTEPATYE PVINSVPPWL VANNLSIGVA 600
    DAPASNESTA GTASSGAAKA DVSMGMVALA AGLGLML 637
    Length:637
    Mass (Da):68,818
    Last modified:February 6, 2013 - v2
    Checksum:i7C052A69669B2276
    GO

    Sequence cautioni

    The sequence AFR98312.2 differs from that shown. Reason: Erroneous gene model prediction.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti6 – 61A → G in AAF65220. (PubMed:11123698)Curated
    Sequence conflicti619 – 6191K → N in AAF65220. (PubMed:11123698)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF223383 Genomic DNA. Translation: AAF65220.1.
    CP003831 Genomic DNA. Translation: AFR98312.2. Sequence problems.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF223383 Genomic DNA. Translation: AAF65220.1 .
    CP003831 Genomic DNA. Translation: AFR98312.2 . Sequence problems.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG7N37NC.

    Family and domain databases

    InterProi IPR016035. Acyl_Trfase/lysoPLipase.
    IPR002642. LysoPLipase_cat_dom.
    [Graphical view ]
    Pfami PF01735. PLA2_B. 1 hit.
    [Graphical view ]
    SMARTi SM00022. PLAc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52151. SSF52151. 1 hit.
    PROSITEi PS51210. PLA2C. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
    2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
      Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
      , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
      PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
    3. "Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans."
      Chen S.C.A., Wright L.C., Golding J.C., Sorrell T.C.
      Biochem. J. 347:431-439(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: BL-1.

    Entry informationi

    Entry nameiPLB1_CRYNH
    AccessioniPrimary (citable) accession number: Q9P8P2
    Secondary accession number(s): J9VV08
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: February 6, 2013
    Last modified: October 1, 2014
    This is version 48 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    PubMed:10749672 describes peptide sequences that do not match PLB1, but originate from a copurified, contaminating chitin deacetylase.Curated

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3