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Q9P8P2

- PLB1_CRYNH

UniProt

Q9P8P2 - PLB1_CRYNH

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Protein

Phospholipase B

Gene
PLB1, CNAG_06085
Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Exhibits phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by Fe3+ ion.

pH dependencei

Active only at acidic pHs.

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. phospholipid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B (EC:3.1.1.5)
Alternative name(s):
Lysophospholipase
Gene namesi
Name:PLB1
ORF Names:CNAG_06085
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000010091: Chromosome 12

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919 Reviewed predictionAdd
BLAST
Chaini20 – 637618Phospholipase BPRO_0000024636Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...) Reviewed prediction
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Glycosylationi122 – 1221N-linked (GlcNAc...) Reviewed prediction
Glycosylationi231 – 2311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi246 – 2461N-linked (GlcNAc...) Reviewed prediction
Glycosylationi272 – 2721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi314 – 3141N-linked (GlcNAc...) Reviewed prediction
Glycosylationi343 – 3431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi387 – 3871N-linked (GlcNAc...) Reviewed prediction
Glycosylationi433 – 4331N-linked (GlcNAc...) Reviewed prediction
Glycosylationi481 – 4811N-linked (GlcNAc...) Reviewed prediction
Glycosylationi501 – 5011N-linked (GlcNAc...) Reviewed prediction
Glycosylationi528 – 5281N-linked (GlcNAc...) Reviewed prediction
Glycosylationi553 – 5531N-linked (GlcNAc...) Reviewed prediction
Glycosylationi572 – 5721N-linked (GlcNAc...) Reviewed prediction
Glycosylationi594 – 5941N-linked (GlcNAc...) Reviewed prediction
Glycosylationi606 – 6061N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 572527PLA2cAdd
BLAST

Sequence similaritiesi

Belongs to the lysophospholipase family.
Contains 1 PLA2c domain.

Keywords - Domaini

Signal

Phylogenomic databases

OrthoDBiEOG7N37NC.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P8P2-1 [UniParc]FASTAAdd to Basket

« Hide

MSIATATFAF SLFATIAFAV PPETPRIELQ AERGLGDKSY APWQVDCPSN    50
VTWIRNATTG LGSGERAYIE AREKLVQPVI EQMMAARGLE TPPRTPNIGV 100
ALSGGGYRAM LTGLGGIMGM MNESTEASES ETGGWLDGVS YWAGLSGGSW 150
ATGTFMSNGG QLPTNLLENL WNIDSNLVFP DDDKLSFYTE LYTETNAKSD 200
LGFPIQITDV WGLAIGSHVL PERYQLSNTP NLTFSSLPSV VSALGNASLP 250
MPIIIAADRK RREAGELVIA ENATVWEFTP YEFGSWAFGS QYKSPGAFTP 300
IEYLGTSVDD GSPNGTCWKG FDQLSFVMGT SATLFNGAFL ELNGTDSGLL 350
TNLITAFLAD LGEDQADISR IPNTFSNYNS GENPIYNLTY ITLVDAGETN 400
QNIPLEPLLV PTRDVDAIVA FDSSYDTDYI WPNGTALRTT YERAKVLAEH 450
ENTRVLMPEV PSMNGFVNGG YNSRPTFFGC NDTTTPLIIY VPSYPWSFAA 500
NTSTYQLSYE NDEANEMLLN GMRSLTLNHS VPTWPTCFAC ALTDRSFMYT 550
SENRSTTCQK CFDTWCWAGD DNTTEPATYE PVINSVPPWL VANNLSIGVA 600
DAPASNESTA GTASSGAAKA DVSMGMVALA AGLGLML 637
Length:637
Mass (Da):68,818
Last modified:February 6, 2013 - v2
Checksum:i7C052A69669B2276
GO

Sequence cautioni

The sequence AFR98312.2 differs from that shown. Reason: Erroneous gene model prediction.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → G in AAF65220. 1 Publication
Sequence conflicti619 – 6191K → N in AAF65220. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF223383 Genomic DNA. Translation: AAF65220.1.
CP003831 Genomic DNA. Translation: AFR98312.2. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF223383 Genomic DNA. Translation: AAF65220.1 .
CP003831 Genomic DNA. Translation: AFR98312.2 . Sequence problems.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OrthoDBi EOG7N37NC.

Family and domain databases

InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 1 hit.
PROSITEi PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
    Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
    , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
    PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  3. "Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans."
    Chen S.C.A., Wright L.C., Golding J.C., Sorrell T.C.
    Biochem. J. 347:431-439(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: BL-1.

Entry informationi

Entry nameiPLB1_CRYNH
AccessioniPrimary (citable) accession number: Q9P8P2
Secondary accession number(s): J9VV08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 6, 2013
Last modified: June 11, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

1 Publication describes peptide sequences that do not match PLB1, but originate from a copurified, contaminating chitin deacetylase.

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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