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Q9P8P2

- PLB1_CRYNH

UniProt

Q9P8P2 - PLB1_CRYNH

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Protein

Phospholipase B

Gene

PLB1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Exhibits phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by Fe3+ ion.

pH dependencei

Active only at acidic pHs.

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC

GO - Biological processi

  1. phospholipid catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B (EC:3.1.1.5)
Alternative name(s):
Lysophospholipase
Gene namesi
Name:PLB1
ORF Names:CNAG_06085
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000010091: Chromosome 12

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence AnalysisAdd
BLAST
Chaini20 – 637618Phospholipase BPRO_0000024636Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi50 – 501N-linked (GlcNAc...)Sequence Analysis
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi122 – 1221N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis
Glycosylationi246 – 2461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi272 – 2721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence Analysis
Glycosylationi343 – 3431N-linked (GlcNAc...)Sequence Analysis
Glycosylationi387 – 3871N-linked (GlcNAc...)Sequence Analysis
Glycosylationi433 – 4331N-linked (GlcNAc...)Sequence Analysis
Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence Analysis
Glycosylationi501 – 5011N-linked (GlcNAc...)Sequence Analysis
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi553 – 5531N-linked (GlcNAc...)Sequence Analysis
Glycosylationi572 – 5721N-linked (GlcNAc...)Sequence Analysis
Glycosylationi594 – 5941N-linked (GlcNAc...)Sequence Analysis
Glycosylationi606 – 6061N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini46 – 572527PLA2cPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

OrthoDBiEOG7N37NC.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P8P2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIATATFAF SLFATIAFAV PPETPRIELQ AERGLGDKSY APWQVDCPSN
60 70 80 90 100
VTWIRNATTG LGSGERAYIE AREKLVQPVI EQMMAARGLE TPPRTPNIGV
110 120 130 140 150
ALSGGGYRAM LTGLGGIMGM MNESTEASES ETGGWLDGVS YWAGLSGGSW
160 170 180 190 200
ATGTFMSNGG QLPTNLLENL WNIDSNLVFP DDDKLSFYTE LYTETNAKSD
210 220 230 240 250
LGFPIQITDV WGLAIGSHVL PERYQLSNTP NLTFSSLPSV VSALGNASLP
260 270 280 290 300
MPIIIAADRK RREAGELVIA ENATVWEFTP YEFGSWAFGS QYKSPGAFTP
310 320 330 340 350
IEYLGTSVDD GSPNGTCWKG FDQLSFVMGT SATLFNGAFL ELNGTDSGLL
360 370 380 390 400
TNLITAFLAD LGEDQADISR IPNTFSNYNS GENPIYNLTY ITLVDAGETN
410 420 430 440 450
QNIPLEPLLV PTRDVDAIVA FDSSYDTDYI WPNGTALRTT YERAKVLAEH
460 470 480 490 500
ENTRVLMPEV PSMNGFVNGG YNSRPTFFGC NDTTTPLIIY VPSYPWSFAA
510 520 530 540 550
NTSTYQLSYE NDEANEMLLN GMRSLTLNHS VPTWPTCFAC ALTDRSFMYT
560 570 580 590 600
SENRSTTCQK CFDTWCWAGD DNTTEPATYE PVINSVPPWL VANNLSIGVA
610 620 630
DAPASNESTA GTASSGAAKA DVSMGMVALA AGLGLML
Length:637
Mass (Da):68,818
Last modified:February 6, 2013 - v2
Checksum:i7C052A69669B2276
GO

Sequence cautioni

The sequence AFR98312.2 differs from that shown. Reason: Erroneous gene model prediction. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61A → G in AAF65220. (PubMed:11123698)Curated
Sequence conflicti619 – 6191K → N in AAF65220. (PubMed:11123698)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223383 Genomic DNA. Translation: AAF65220.1.
CP003831 Genomic DNA. Translation: AFR98312.2. Sequence problems.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223383 Genomic DNA. Translation: AAF65220.1 .
CP003831 Genomic DNA. Translation: AFR98312.2 . Sequence problems.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OrthoDBi EOG7N37NC.

Family and domain databases

InterProi IPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view ]
Pfami PF01735. PLA2_B. 1 hit.
[Graphical view ]
SMARTi SM00022. PLAc. 1 hit.
[Graphical view ]
SUPFAMi SSF52151. SSF52151. 1 hit.
PROSITEi PS51210. PLA2C. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  2. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
    Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
    , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
    PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  3. "Purification and characterization of secretory phospholipase B, lysophospholipase and lysophospholipase/transacylase from a virulent strain of the pathogenic fungus Cryptococcus neoformans."
    Chen S.C.A., Wright L.C., Golding J.C., Sorrell T.C.
    Biochem. J. 347:431-439(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: BL-1.

Entry informationi

Entry nameiPLB1_CRYNH
AccessioniPrimary (citable) accession number: Q9P8P2
Secondary accession number(s): J9VV08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 6, 2013
Last modified: October 29, 2014
This is version 49 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

PubMed:10749672 describes peptide sequences that do not match PLB1, but originate from a copurified, contaminating chitin deacetylase.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3