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Protein

Phospholipase B

Gene

PLB1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Exhibits phospholipase B (PLB), lysophospholipase (LPL) and lysophospholipase/transacylase (LPTA) activities.

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by Fe3+ ion.

pH dependencei

Active only at acidic pHs.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B (EC:3.1.1.5)
Alternative name(s):
Lysophospholipase
Gene namesi
Name:PLB1
ORF Names:CNAG_06085
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000010091 Componenti: Chromosome 12

Organism-specific databases

EuPathDBiFungiDB:CNAG_06085.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19Sequence analysisAdd BLAST19
ChainiPRO_000002463620 – 637Phospholipase BAdd BLAST618

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi50N-linked (GlcNAc...)Sequence analysis1
Glycosylationi56N-linked (GlcNAc...)Sequence analysis1
Glycosylationi122N-linked (GlcNAc...)Sequence analysis1
Glycosylationi231N-linked (GlcNAc...)Sequence analysis1
Glycosylationi246N-linked (GlcNAc...)Sequence analysis1
Glycosylationi272N-linked (GlcNAc...)Sequence analysis1
Glycosylationi314N-linked (GlcNAc...)Sequence analysis1
Glycosylationi343N-linked (GlcNAc...)Sequence analysis1
Glycosylationi387N-linked (GlcNAc...)Sequence analysis1
Glycosylationi433N-linked (GlcNAc...)Sequence analysis1
Glycosylationi481N-linked (GlcNAc...)Sequence analysis1
Glycosylationi501N-linked (GlcNAc...)Sequence analysis1
Glycosylationi528N-linked (GlcNAc...)Sequence analysis1
Glycosylationi553N-linked (GlcNAc...)Sequence analysis1
Glycosylationi572N-linked (GlcNAc...)Sequence analysis1
Glycosylationi594N-linked (GlcNAc...)Sequence analysis1
Glycosylationi606N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

N-glycosylated.

Keywords - PTMi

Glycoprotein

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 572PLA2cPROSITE-ProRule annotationAdd BLAST527

Sequence similaritiesi

Belongs to the lysophospholipase family.Curated
Contains 1 PLA2c domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

OrthoDBiEOG092C2IZ9.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P8P2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIATATFAF SLFATIAFAV PPETPRIELQ AERGLGDKSY APWQVDCPSN
60 70 80 90 100
VTWIRNATTG LGSGERAYIE AREKLVQPVI EQMMAARGLE TPPRTPNIGV
110 120 130 140 150
ALSGGGYRAM LTGLGGIMGM MNESTEASES ETGGWLDGVS YWAGLSGGSW
160 170 180 190 200
ATGTFMSNGG QLPTNLLENL WNIDSNLVFP DDDKLSFYTE LYTETNAKSD
210 220 230 240 250
LGFPIQITDV WGLAIGSHVL PERYQLSNTP NLTFSSLPSV VSALGNASLP
260 270 280 290 300
MPIIIAADRK RREAGELVIA ENATVWEFTP YEFGSWAFGS QYKSPGAFTP
310 320 330 340 350
IEYLGTSVDD GSPNGTCWKG FDQLSFVMGT SATLFNGAFL ELNGTDSGLL
360 370 380 390 400
TNLITAFLAD LGEDQADISR IPNTFSNYNS GENPIYNLTY ITLVDAGETN
410 420 430 440 450
QNIPLEPLLV PTRDVDAIVA FDSSYDTDYI WPNGTALRTT YERAKVLAEH
460 470 480 490 500
ENTRVLMPEV PSMNGFVNGG YNSRPTFFGC NDTTTPLIIY VPSYPWSFAA
510 520 530 540 550
NTSTYQLSYE NDEANEMLLN GMRSLTLNHS VPTWPTCFAC ALTDRSFMYT
560 570 580 590 600
SENRSTTCQK CFDTWCWAGD DNTTEPATYE PVINSVPPWL VANNLSIGVA
610 620 630
DAPASNESTA GTASSGAAKA DVSMGMVALA AGLGLML
Length:637
Mass (Da):68,818
Last modified:February 6, 2013 - v2
Checksum:i7C052A69669B2276
GO

Sequence cautioni

The sequence AFR98312 differs from that shown. Reason: Erroneous gene model prediction.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6A → G in AAF65220 (PubMed:11123698).Curated1
Sequence conflicti619K → N in AAF65220 (PubMed:11123698).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223383 Genomic DNA. Translation: AAF65220.1.
CP003831 Genomic DNA. Translation: AFR98312.2. Sequence problems.
RefSeqiXP_012052996.1. XM_012197606.1.

Genome annotation databases

EnsemblFungiiAFR98312; AFR98312; CNAG_06085.
GeneIDi23889325.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF223383 Genomic DNA. Translation: AAF65220.1.
CP003831 Genomic DNA. Translation: AFR98312.2. Sequence problems.
RefSeqiXP_012052996.1. XM_012197606.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiAFR98312; AFR98312; CNAG_06085.
GeneIDi23889325.

Organism-specific databases

EuPathDBiFungiDB:CNAG_06085.

Phylogenomic databases

OrthoDBiEOG092C2IZ9.

Family and domain databases

InterProiIPR016035. Acyl_Trfase/lysoPLipase.
IPR002642. LysoPLipase_cat_dom.
[Graphical view]
PfamiPF01735. PLA2_B. 1 hit.
[Graphical view]
SMARTiSM00022. PLAc. 1 hit.
[Graphical view]
SUPFAMiSSF52151. SSF52151. 1 hit.
PROSITEiPS51210. PLA2C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPLB1_CRYNH
AccessioniPrimary (citable) accession number: Q9P8P2
Secondary accession number(s): J9VV08
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: February 6, 2013
Last modified: November 30, 2016
This is version 55 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

PubMed:10749672 describes peptide sequences that do not match PLB1, but originate from a copurified, contaminating chitin deacetylase.Curated

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.