Reviewed, UniProtKB/Swiss-Prot Q9P8N0 (MPG1_PICAN)

Names and origin

Protein names

Recommended name:
    Mannose-1-phosphate guanyltransferase
    EC=2.7.7.13
Alternative name(s):
    GTP-mannose-1-phosphate guanylyltransferase
    GDP-mannose pyrophosphorylase

Gene names

Name:	MPG1
Synonyms:	OPU24

Organism

Pichia angusta (Yeast) (Hansenula polymorpha)

Taxonomic identifier

4905 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Pichia

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell-size checkpoint. Ref.1
Catalytic activity	GTP + alpha-D-mannose 1-phosphate = diphosphate + GDP-mannose.
Pathway	Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; GDP-alpha-D-mannose from alpha-D-mannose 1-phosphate (GTP route): step 1/1.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the transferase hexapeptide repeat family.

Ontologies

Keywords
Biological process	Cell cycle
Cellular component	Cytoplasm
Ligand	GTP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Gene Ontology (GO)
Biological process	biosynthetic process Inferred from electronic annotation. Source: InterPro cell cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW mannose-1-phosphate guanylyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	Mannose-1-phosphate guanyltransferase		PRO_0000238492

Sequences

	Sequence		Length	Mass (Da)
	Q9P8N0-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 1700BCA9AF0308FD	FASTA	364	40,065
	10 20 30 40 50 60 MKGLILVGGY GTRLRPLTLS LPKPLVEFGN RPMILHQIEA LANAGCTDIV LAVNYKPEVM 70 80 90 100 110 120 VGALKQYEKE YGVSITFSVE EEPLGTAGPL KLAEKILKKD NTPIFVLNSD VICEYPLRDL 130 140 150 160 170 180 LEFHTAHGGE ATIVATKVDE PSKYGVIVHD RDVPNLIERF VEKPVEFVGN RINAGIYVLN 190 200 210 220 230 240 PSVIDLIEMR PTSIEHETFP ILVEQKKLYS FDLPGYWMDV GQPKDFLSGM CLYLSALTKK 250 260 270 280 290 300 NSNLLTSTSE EYVNGGNVLI DPSAKIGKGC KIGPNVVIGP NCIIGDGVRI QRSTILKNSQ 310 320 330 340 350 360 IKDHAWVKST IVGWNSTVGK WARLEGVTVL GEDVTVKDEV YVNGGKVLPH KSIKDNVETP QIIM

References

[1]

"Mutation of the homologue of GDP-mannose pyrophosphorylase alters cell wall structure, protein glycosylation and secretion in Hansenula polymorpha." Agaphonov M.O., Packeiser A.N., Chechenova M.B., Choi E.-S., Ter-Avanesyan M.D. Yeast 18:391-402(2001) [PubMed: 11255248] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF234177 Genomic DNA. Translation: AAF60300.1.
3D structure databases
HSSP	HSSP built from PDB template 1HM0 based on UniProtKB Q97R46.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.7.7.13. 276392.
Family and domain databases
InterPro	IPR001451. Hexapep_transf. IPR018357. Hexapep_transf_CS. IPR005835. NTP_transferase. [Graphical view]
Pfam	PF00132. Hexapep. 4 hits. PF00483. NTP_transferase. 1 hit. [Graphical view]
PROSITE	PS00101. HEXAPEP_TRANSFERASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MPG1_PICAN

Accession

Primary (citable) accession number: Q9P8N0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	October 1, 2000
Last modified:	September 22, 2009
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)