Reviewed, UniProtKB/Swiss-Prot Q9P8I0 (PUT2_EMENI)

Names and origin

Protein names

Recommended name:
    Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial
      Short name=P5C dehydrogenase
    EC=1.5.1.12

Gene names

Name:	prnC
ORF Names:	AN1733

Organism

Emericella nidulans (Aspergillus nidulans)

Taxonomic identifier

162425 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › Emericella

Protein attributes

Sequence length	572 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-1-pyrroline-5-carboxylate + NAD(P)+ + 2 H2O = L-glutamate + NAD(P)H.
Pathway	Amino-acid degradation; L-proline degradation into L-glutamate; L-glutamate from L-proline: step 2/2.
Subcellular location	Mitochondrion matrix.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Biological process	Proline metabolism
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	1-pyrroline-5-carboxylate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 572		Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial		PRO_0000007176
Regions
	Nucleotide binding	300 – 305	6	NAD By similarity
Sites
	Active site	320	1	Proton acceptor By similarity
	Active site	354	1	Nucleophile By similarity
	Site	215	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	149	1	L → V in AAF72527. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9P8I0-1 [UniParc]. Last modified May 1, 2007. Version 2. Checksum: 249FBB3461A9C3DD	FASTA	572	61,936
	10 20 30 40 50 60 MQSSMLLRVR ALPKTASVLS RTKTATYATY KVPRIDNEPN KHYAAGSPDR KALQEALART 70 80 90 100 110 120 QRNAPLSVPL VIAGKEVKSS SSLTQSNPAS HGPVATYSNA TAKDVQAAIE SALEARKSWA 130 140 150 160 170 180 STPFADRASV FLKAADLIST KYRYDVMALT MHGQGKNAWQ AEIDSAAELC DFFRFGVKYA 190 200 210 220 230 240 EDLYAQQPVH HAPGVWNRVE YRPLEGFVYA ISPFNFTAIG GNLAGAPALM GNVVVWKPSP 250 260 270 280 290 300 SAIASNWLVH QILLEAGLPK NVIQFVPGEA EEVTKTVLDH PDFAALHFTG STNVFRNLYG 310 320 330 340 350 360 QISTRVAAGK YRSYPRIVGE TGGKNFHLIH KSADIRNAAV QTVRGAFEYQ GQKCSATSRV 370 380 390 400 410 420 YVASSIADSF LEQVASEAKS LKVGPPSDFT NFCGPVIHEA SFTKLAKVID EAKNDPELEL 430 440 450 460 470 480 LAGGSYDSSK GWYIQPTVYR TTNPDHPLLT RELFGPILVV YAYPDATEAD FARIAQKIDA 490 500 510 520 530 540 TGEYGLTGSV FAQDREALAV ANDVLRNAAG NFYINCKSTG AVVGQQPFGG ARASGTNDKA 550 560 570 GSGNLLSRFV SLRSIKEEFV PTYKVAYPSN EA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the nuclear prnC gene involved in the mitochondrial pathway for proline utilization in Aspergillus nidulans." Demais S., Gavrias V., Scazzocchio C. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Sequencing of Aspergillus nidulans and comparative analysis with A. fumigatus and A. oryzae." Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S., Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V., Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S., Braus G.H. , Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H., Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K., Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R., Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C., Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L., Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W. Nature 438:1105-1115(2005) [PubMed: 16372000] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: FGSC 4.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF252630 Genomic DNA. Translation: AAF72527.1. AACD01000027 Genomic DNA. Translation: EAA64019.1.
RefSeq	XP_659337.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2875278.
KEGG	ani:AN1733.2.
Enzyme and pathway databases
BRENDA	1.5.1.12. 3859.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. IPR005931. d-1-pyrroline-5-COlate_DH-1. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
TIGRFAMs	TIGR01236. D1pyr5carbox1. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUT2_EMENI

Accession

Primary (citable) accession number: Q9P8I0
Secondary accession number(s): Q5BCJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2003
Last sequence update:	May 1, 2007
Last modified:	June 16, 2009
This is version 48 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)