Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Two-component system protein A

Gene

tcsA

Organism
Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

May be part of a two-component regulatory system required for formation of conidia on certain growth media.Curated1 Publication

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

GO - Molecular functioni

GO - Biological processi

  • ascospore formation Source: UniProtKB
  • conidium formation Source: ASPGD
  • phosphorelay signal transduction system Source: UniProtKB
  • regulation of conidium formation Source: ASPGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Conidiation, Sporulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Two-component system protein A (EC:2.7.13.3)
Gene namesi
Name:tcsAImported
ORF Names:AN5296
OrganismiEmericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139) (Aspergillus nidulans)
Taxonomic identifieri227321 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaEurotiomycetesEurotiomycetidaeEurotialesAspergillaceaeAspergillus
Proteomesi
  • UP000000560 Componenti: Chromosome V
  • UP000005890 Componenti: Partially assembled WGS sequence

Organism-specific databases

EuPathDBiFungiDB:AN5296.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 682682Two-component system protein APRO_0000081411Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei310 – 3101Phosphohistidine; by autocatalysisPROSITE-ProRule annotation
Modified residuei615 – 61514-aspartylphosphatePROSITE-ProRule annotation

Post-translational modificationi

Activation probably requires a transfer of a phosphate group between a His in the histidine kinase domain and an Asp of the response regulatory domain.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliQ9P896.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini45 – 10561PAS 1PROSITE-ProRule annotationAdd
BLAST
Domaini166 – 23974PAS 2PROSITE-ProRule annotationAdd
BLAST
Domaini241 – 29252PACPROSITE-ProRule annotationAdd
BLAST
Domaini307 – 530224Histidine kinasePROSITE-ProRule annotationAdd
BLAST
Domaini563 – 680118Response regulatoryPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.PROSITE-ProRule annotation
Contains 1 response regulatory domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000216303.
InParanoidiQ9P896.
KOiK19692.
OMAiGCASCTE.
OrthoDBiEOG092C01BG.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013656. PAS_4.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF08448. PAS_4. 1 hit.
PF13426. PAS_9. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P896-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLNGQISAL SLDDNDNGQQ HQDEVQAKHQ DQGHTCPSRP SVPSLSRIYR
60 70 80 90 100
CTPVPTIVLD ASMVIIEVSN SHVALFGKPR DSLLHTSISD VSPECIPVPN
110 120 130 140 150
IPILYGALRA ACSTREIQVV EHVVVGEKIA HNLRVTPVYE DETLLFVVLE
160 170 180 190 200
VENLRAEVIN NQHAYMNETY KILVDTVKDY AIFMLDPTGH IATWNAGAGV
210 220 230 240 250
LKGYKAEEII GKHFSILYSP ADRDNGKPAR ALDVCLREGR IEDEGWRYRR
260 270 280 290 300
DGSRFWANVL ITPIYQFGQH VGFVKVTRDL TERKEAEACM IAAFEESSRL
310 320 330 340 350
KTDFLANISH EIRTPMNGMQ IALTMLTDTG LSEEQREHAN IVQDSMSLLL
360 370 380 390 400
QIVNDVLDYS KLSSGSFSLH ADMLDIREIV GAVVRNCRSS LQEGVELDTE
410 420 430 440 450
ISPKLPTRMR GDPLRYRQVL QNLVGNAVKF TEKGSIHVKI TSSTDEEDSD
460 470 480 490 500
SSVVRTEVTD TGIGVPDSAI NTLFTPFSRF ANSAARKYQG TGLGLSICKS
510 520 530 540 550
LAELMDGSVG YSPNPNASGS VFWFTAKMGG RSVTPPSKSP SVSGSPVPTE
560 570 580 590 600
VASEMRSIAP RKHVLLVEDN IVNHTVMLKL LHTIGFQRID GAWNGAEAVR
610 620 630 640 650
MVRQKPLSYD IILMDVSMPV LDGLAATEQI RDMGLTMPII AITGNAMKGD
660 670 680
AETYIAQGMD DCICKPVHRD QLLRVLWKWF GT
Length:682
Mass (Da):75,316
Last modified:May 26, 2009 - v2
Checksum:i29D37B021170964D
GO

Sequence cautioni

The sequence CAB93498 differs from that shown. Reason: Frameshift at several positions. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti217 – 2193LYS → SIV in CAB93498 (PubMed:10905426).Curated
Sequence conflicti319 – 3202MQ → IE in CAB93498 (PubMed:10905426).Curated
Sequence conflicti336 – 3383REH → GD in CAB93498 (PubMed:10905426).Curated
Sequence conflicti364 – 3674SGSF → IRLL in CAB93498 (PubMed:10905426).Curated
Sequence conflicti406 – 4116PTRMRG → HAHAW in CAB93498 (PubMed:10905426).Curated
Sequence conflicti472 – 4798TLFTPFSR → HSLHPLLA in CAB93498 (PubMed:10905426).Curated
Sequence conflicti547 – 5537VPTEVAS → YPRSCF in CAB93498 (PubMed:10905426).Curated
Sequence conflicti576 – 5849VMLKLLHTI → RYVEAPTYQF in CAB93498 (PubMed:10905426).Curated
Sequence conflicti682 – 6821T → RSPQSQHGYWTETRRYAFIC MSWRQERYHGKSIYAAYRDL in CAB93498 (PubMed:10905426).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271843 Genomic DNA. Translation: CAB93498.1. Frameshift.
AACD01000093 Genomic DNA. Translation: EAA62456.1.
BN001305 Genomic DNA. Translation: CBF82150.1.
RefSeqiXP_662900.1. XM_657808.1.

Genome annotation databases

EnsemblFungiiCADANIAT00003803; CADANIAP00003803; CADANIAG00003803.
EAA62456; EAA62456; AN5296.2.
GeneIDi2871585.
KEGGiani:AN5296.2.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ271843 Genomic DNA. Translation: CAB93498.1. Frameshift.
AACD01000093 Genomic DNA. Translation: EAA62456.1.
BN001305 Genomic DNA. Translation: CBF82150.1.
RefSeqiXP_662900.1. XM_657808.1.

3D structure databases

ProteinModelPortaliQ9P896.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiCADANIAT00003803; CADANIAP00003803; CADANIAG00003803.
EAA62456; EAA62456; AN5296.2.
GeneIDi2871585.
KEGGiani:AN5296.2.

Organism-specific databases

EuPathDBiFungiDB:AN5296.

Phylogenomic databases

HOGENOMiHOG000216303.
InParanoidiQ9P896.
KOiK19692.
OMAiGCASCTE.
OrthoDBiEOG092C01BG.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR011006. CheY-like_superfamily.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR001610. PAC.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR013656. PAS_4.
IPR004358. Sig_transdc_His_kin-like_C.
IPR001789. Sig_transdc_resp-reg_receiver.
[Graphical view]
PfamiPF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF08448. PAS_4. 1 hit.
PF13426. PAS_9. 1 hit.
PF00072. Response_reg. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
SM00448. REC. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF52172. SSF52172. 1 hit.
SSF55785. SSF55785. 2 hits.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
PS50110. RESPONSE_REGULATORY. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTCSA_EMENI
AccessioniPrimary (citable) accession number: Q9P896
Secondary accession number(s): C8VGY4, Q5B2D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: May 26, 2009
Last modified: September 7, 2016
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.