Reviewed, UniProtKB/Swiss-Prot Q9P885 (GGPPS_RHIRA)

Names and origin

Protein names

Recommended name:
    Geranylgeranyl pyrophosphate synthetase
      Short name=GGPP synthetase
      Short name=GGPPSase
Alternative name(s):
    Geranylgeranyl diphosphate synthase
Including the following 3 domains:
    1- Recommended name:
            Dimethylallyltranstransferase
              EC=2.5.1.1
    2- Recommended name:
            Geranyltranstransferase
              EC=2.5.1.10
    3- Recommended name:
            Farnesyltranstransferase
              EC=2.5.1.29

Gene names

Name:

carG

Organism

Rhizomucor racemosus (Mucor circinelloides f. lusitanicus)

Taxonomic identifier

4841 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Fungi incertae sedis › Basal fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Mucor

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the trans-addition of the three molecules of IPP onto DMAPP to form geranylgeranyl pyrophosphate.
Catalytic activity	Dimethylallyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate. Ref.1 Geranyl diphosphate + isopentenyl diphosphate = diphosphate + trans,trans-farnesyl diphosphate. Ref.1 Trans,trans-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate. Ref.1
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Pathway	Isoprenoid biosynthesis; farnesyl diphosphate biosynthesis; farnesyl diphosphate from geranyl diphosphate and isopentenyl diphosphate: step 1/1. Ref.1 Isoprenoid biosynthesis; geranyl diphosphate biosynthesis; geranyl diphosphate from dimethylallyl diphosphate and isopentenyl diphosphate: step 1/1. Ref.1 Isoprenoid biosynthesis; geranylgeranyl diphosphate biosynthesis; geranylgeranyl diphosphate from farnesyl diphosphate and isopentenyl diphosphate: step 1/1. Ref.1
Subcellular location	Cytoplasm.
Induction	By blue light. Ref.1
Sequence similarities	Belongs to the FPP/GGPP synthetase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis Isoprene biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological process	carotenoid biosynthetic process Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell soluble fraction Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	dimethylallyltranstransferase activity Inferred from electronic annotation. Source: EC farnesyltranstransferase activity Ref.1 Inferred from direct assay. Source: UniProtKB geranyltranstransferase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 303	303	Geranylgeranyl pyrophosphate synthetase		PRO_0000123967
Sites
	Metal binding	69	1	Magnesium 1 By similarity
	Metal binding	69	1	Magnesium 2 By similarity
	Metal binding	73	1	Magnesium 1 By similarity
	Metal binding	73	1	Magnesium 2 By similarity
	Metal binding	193	1	Magnesium 3 By similarity
	Binding site	30	1	Isopentenyl diphosphate By similarity
	Binding site	33	1	Isopentenyl diphosphate By similarity
	Binding site	62	1	Isopentenyl diphosphate By similarity
	Binding site	78	1	Dimethylallyl diphosphate By similarity
	Binding site	79	1	Isopentenyl diphosphate By similarity
	Binding site	156	1	Dimethylallyl diphosphate By similarity
	Binding site	157	1	Dimethylallyl diphosphate By similarity
	Binding site	190	1	Dimethylallyl diphosphate By similarity
	Binding site	207	1	Dimethylallyl diphosphate By similarity
	Binding site	217	1	Dimethylallyl diphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9P885-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: D440757CA7D0E215	FASTA	303	34,787
	10 20 30 40 50 60 MLNSHNRTEE RSTEDIILEP YTYLISQPGK DIRAKLISAF DLWLHVPKDV LCVINKIIGM 70 80 90 100 110 120 LHNASLMIDD VQDDSDLRRG VPVAHHIYGV PQTINTANYV IFLALQEVMK LNIPSMMQVC 130 140 150 160 170 180 TEELINLHRG QGIELYWRDS LTCPTEEEYI DMVNNKTSGL LRLAVRLMQA ASESDIDYTP 190 200 210 220 230 240 LVNIIGIHFQ VRDDYMNLQS TSYTNNKGFC EDLTEGKFSF PIIHAIRKDP SNRQLLNIIS 250 260 270 280 290 300 QKPTSIEVKK YALEVIRKAG SFEYVREFLR QKEAESLKEI KRLGGNPLLE KYIETIRVEA TND

References

[1]

"Expression of the carG gene, encoding geranylgeranyl pyrophosphate synthase, is up-regulated by blue light in Mucor circinelloides." Velayos A., Papp T., Aguilar-Elena R., Fuentes-Vicente M., Eslava A.P., Iturriaga E.A., Alvarez M.I. Curr. Genet. 43:112-120(2003) [PubMed: 12695851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, PATHWAY, INDUCTION. Strain: ATCC 1216b / CBS 277.49 / NRRL 3631. Tissue: Mycelium.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ276129 Genomic DNA. Translation: CAB89115.1.
3D structure databases
SMR	Q9P885. Positions 12-295.
ModBase	Search...
Family and domain databases
InterPro	IPR000092. Polyprenyl_synt. IPR017446. Polyprenyl_synth-rel. IPR008949. Terpenoid_synth. [Graphical view]
Gene3D	G3DSA:1.10.600.10. Terpenoid_synth. 1 hit.
PANTHER	PTHR12001. Polyprenyl_synt. 1 hit.
Pfam	PF00348. polyprenyl_synt. 1 hit. [Graphical view]
PROSITE	PS00723. POLYPRENYL_SYNTHET_1. 1 hit. PS00444. POLYPRENYL_SYNTHET_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GGPPS_RHIRA

Accession

Primary (citable) accession number: Q9P885

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 12, 2005
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)