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Q9P854

- LCPS_PHYB8

UniProt

Q9P854 - LCPS_PHYB8

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Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

carRA

Organism
Phycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).1 Publication

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
  2. intramolecular lyase activity Source: InterPro
  3. lycopene beta cyclase activity Source: UniProtKB
  4. phytoene synthase activity Source: UniProtKB

GO - Biological processi

  1. carotene biosynthetic process Source: UniProtKB
  2. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Alternative name(s):
Lycopene cyclase
Phytoene synthase (EC:2.5.1.32)
Gene namesi
Name:carRA
OrganismiPhycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Taxonomic identifieri763407 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771E → K in carR23; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. 1 Publication
Mutagenesisi215 – 2151P → S in carR21; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. 1 Publication
Mutagenesisi465 – 4651I → T in carA113; reduces the response to chemical activation. 1 Publication
Mutagenesisi482 – 4821P → L in carA5; abolishes phytoene synthase activity resulting in a 10- to 15-fold reduction of the beta-carotene content. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Bifunctional lycopene cyclase/phytoene synthasePRO_0000409240Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9P854.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721HelicalSequence AnalysisAdd
BLAST
Transmembranei35 – 5521HelicalSequence AnalysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence AnalysisAdd
BLAST
Transmembranei110 – 13021HelicalSequence AnalysisAdd
BLAST
Transmembranei142 – 16221HelicalSequence AnalysisAdd
BLAST
Transmembranei173 – 19321HelicalSequence AnalysisAdd
BLAST
Transmembranei211 – 23121HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 238238Lycopene beta-cyclaseAdd
BLAST
Regioni245 – 602358Phytoene synthaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P854-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MLTYMEVHLY FTLPVLALLA FLYKPFFTTK DRFKYIFLCT VAFATASPWD
60 70 80 90 100
NYIVYHKAWS YCPECVTAVI GYVPLEEYMF FIIMTLITVT FTSLTMRWTL
110 120 130 140 150
PSFFIRPETP VFQSVCVRYI PIVGFLTIAA KAWASSIPDS HPFYGACILW
160 170 180 190 200
YVCPVLALLW IGSGEYMLRR WKAVLFSIAV PTIFLCWVDQ YAIARGTWDI
210 220 230 240 250
SRRTSTGIMV LPSLPLEEFL FFLLIDTVLV FASCATDRAH AIVHIYITPM
260 270 280 290 300
NHNKVSTWYM DFFYLCWAFL QTDQALSGET LSDLDATWRI LREASASFYT
310 320 330 340 350
ASSVFSFEAR QDLGVLYGFC RATDDLADNN DVSVPDRKKQ LELVRGFVRQ
360 370 380 390 400
MFDSKHGHPD IDWTQYSGSI PDSFIAAFRS FTRLRDVLEI KAVEELLDGY
410 420 430 440 450
TFDLEQREVK NEDDLVYYSA CVASSVGEMC TRVLMASEPG GNRTMLKWTV
460 470 480 490 500
ERARDMGLAL QLTNIARDIV TDSKQLGRSY VPRDWLTSQE SALLKAGKAR
510 520 530 540 550
ELGDERLRQI ALKMVYTADD LNLMASRAID YLPPSSRCGV RAACNVYTAI
560 570 580 590 600
GVSLHKANGY PDRAHLTKLE RMKVTFRCVY GFRKGHQGVQ GDRGKSQAFT

VI
Length:602
Mass (Da):68,670
Last modified:October 1, 2000 - v1
Checksum:iFF2C7525405D7F99
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti436 – 4361A → G in CAB86388. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ278287 Genomic DNA. Translation: CAB93661.1.
AJ276965 Genomic DNA. Translation: CAB86388.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ278287 Genomic DNA. Translation: CAB93661.1 .
AJ276965 Genomic DNA. Translation: CAB86388.1 .

3D structure databases

ProteinModelPortali Q9P854.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00799 ; UER00773 .
UPA00802 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00494. SQS_PSY. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
TIGRFAMsi TIGR03462. CarR_dom_SF. 2 hits.
PROSITEi PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces."
    Arrach N., Fernandez-Martin R., Cerda-Olmedo E., Avalos J.
    Proc. Natl. Acad. Sci. U.S.A. 98:1687-1692(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-77; PRO-215; ILE-465 AND PRO-482, FUNCTION.
    Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.
  2. "The carRA gene of Phycomyces blakesleeanus."
    Sanz C.S., Benito E.P., Eslava A.P.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

Entry informationi

Entry nameiLCPS_PHYB8
AccessioniPrimary (citable) accession number: Q9P854
Secondary accession number(s): Q9P877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 1, 2000
Last modified: October 1, 2014
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3