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Q9P854

- LCPS_PHYB8

UniProt

Q9P854 - LCPS_PHYB8

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Protein
Bifunctional lycopene cyclase/phytoene synthase
Gene
carRA
Organism
Phycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).1 Publication

Catalytic activityi

Carotenoid psi-end group = carotenoid beta-end group.
2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathwayi

GO - Molecular functioni

  1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
  2. intramolecular lyase activity Source: InterPro
  3. lycopene beta cyclase activity Source: UniProtKB
  4. phytoene synthase activity Source: UniProtKB

GO - Biological processi

  1. carotene biosynthetic process Source: UniProtKB
  2. carotenoid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Transferase

Keywords - Biological processi

Carotenoid biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00799; UER00773.
UPA00802.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional lycopene cyclase/phytoene synthase
Including the following 2 domains:
Lycopene beta-cyclase (EC:5.5.1.19)
Alternative name(s):
Lycopene cyclase
Phytoene synthase (EC:2.5.1.32)
Gene namesi
Name:carRA
OrganismiPhycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Taxonomic identifieri763407 [NCBI]
Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei7 – 2721Helical; Reviewed prediction
Add
BLAST
Transmembranei35 – 5521Helical; Reviewed prediction
Add
BLAST
Transmembranei69 – 8921Helical; Reviewed prediction
Add
BLAST
Transmembranei110 – 13021Helical; Reviewed prediction
Add
BLAST
Transmembranei142 – 16221Helical; Reviewed prediction
Add
BLAST
Transmembranei173 – 19321Helical; Reviewed prediction
Add
BLAST
Transmembranei211 – 23121Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi77 – 771E → K in carR23; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. 1 Publication
Mutagenesisi215 – 2151P → S in carR21; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. 1 Publication
Mutagenesisi465 – 4651I → T in carA113; reduces the response to chemical activation. 1 Publication
Mutagenesisi482 – 4821P → L in carA5; abolishes phytoene synthase activity resulting in a 10- to 15-fold reduction of the beta-carotene content. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 602602Bifunctional lycopene cyclase/phytoene synthase
PRO_0000409240Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliQ9P854.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 238238Lycopene beta-cyclase
Add
BLAST
Regioni245 – 602358Phytoene synthase
Add
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the lycopene beta-cyclase family.
In the C-terminal section; belongs to the phytoene/squalene synthase family.

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

Gene3Di1.10.600.10. 1 hit.
InterProiIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamiPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMiSSF48576. SSF48576. 1 hit.
TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P854-1 [UniParc]FASTAAdd to Basket

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MLTYMEVHLY FTLPVLALLA FLYKPFFTTK DRFKYIFLCT VAFATASPWD    50
NYIVYHKAWS YCPECVTAVI GYVPLEEYMF FIIMTLITVT FTSLTMRWTL 100
PSFFIRPETP VFQSVCVRYI PIVGFLTIAA KAWASSIPDS HPFYGACILW 150
YVCPVLALLW IGSGEYMLRR WKAVLFSIAV PTIFLCWVDQ YAIARGTWDI 200
SRRTSTGIMV LPSLPLEEFL FFLLIDTVLV FASCATDRAH AIVHIYITPM 250
NHNKVSTWYM DFFYLCWAFL QTDQALSGET LSDLDATWRI LREASASFYT 300
ASSVFSFEAR QDLGVLYGFC RATDDLADNN DVSVPDRKKQ LELVRGFVRQ 350
MFDSKHGHPD IDWTQYSGSI PDSFIAAFRS FTRLRDVLEI KAVEELLDGY 400
TFDLEQREVK NEDDLVYYSA CVASSVGEMC TRVLMASEPG GNRTMLKWTV 450
ERARDMGLAL QLTNIARDIV TDSKQLGRSY VPRDWLTSQE SALLKAGKAR 500
ELGDERLRQI ALKMVYTADD LNLMASRAID YLPPSSRCGV RAACNVYTAI 550
GVSLHKANGY PDRAHLTKLE RMKVTFRCVY GFRKGHQGVQ GDRGKSQAFT 600
VI 602
Length:602
Mass (Da):68,670
Last modified:October 1, 2000 - v1
Checksum:iFF2C7525405D7F99
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti436 – 4361A → G in CAB86388. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ278287 Genomic DNA. Translation: CAB93661.1.
AJ276965 Genomic DNA. Translation: CAB86388.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ278287 Genomic DNA. Translation: CAB93661.1 .
AJ276965 Genomic DNA. Translation: CAB86388.1 .

3D structure databases

ProteinModelPortali Q9P854.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00799 ; UER00773 .
UPA00802 .

Family and domain databases

Gene3Di 1.10.600.10. 1 hit.
InterProi IPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view ]
Pfami PF00494. SQS_PSY. 1 hit.
[Graphical view ]
SUPFAMi SSF48576. SSF48576. 1 hit.
TIGRFAMsi TIGR03462. CarR_dom_SF. 2 hits.
PROSITEi PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces."
    Arrach N., Fernandez-Martin R., Cerda-Olmedo E., Avalos J.
    Proc. Natl. Acad. Sci. U.S.A. 98:1687-1692(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-77; PRO-215; ILE-465 AND PRO-482, FUNCTION.
    Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.
  2. "The carRA gene of Phycomyces blakesleeanus."
    Sanz C.S., Benito E.P., Eslava A.P.
    Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

Entry informationi

Entry nameiLCPS_PHYB8
AccessioniPrimary (citable) accession number: Q9P854
Secondary accession number(s): Q9P877
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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