Q9P854 (LCPS_PHYB8) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

1. Lycopene beta-cyclase
   EC=5.5.1.19
   Alternative name(s):
   Lycopene cyclase
2. Phytoene synthase
   EC=2.5.1.32

Gene names

Name:

carRA

Organism

Phycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)

Taxonomic identifier

763407 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Fungi incertae sedis › Early diverging fungal lineages › Mucoromycotina › Mucorales › Mucoraceae › Phycomyces › Phycomyces blakesleeanus

Protein attributes

Sequence length	602 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase). Ref.1
Catalytic activity	Carotenoid psi-end group = carotenoid beta-end group. 2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.
Pathway	Carotenoid biosynthesis; beta-carotene biosynthesis. Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	In the N-terminal section; belongs to the lycopene beta-cyclase family. In the C-terminal section; belongs to the phytoene/squalene synthase family.

Ontologies

Keywords
Biological process	Carotenoid biosynthesis
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Isomerase Transferase
Technical term	Multifunctional enzyme
Gene Ontology (GO)
Biological_process	carotenoid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	geranylgeranyl-diphosphate geranylgeranyltransferase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB intramolecular lyase activity Inferred from electronic annotation. Source: InterPro lycopene beta cyclase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB phytoene synthase activity Inferred from mutant phenotype Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 602	602	Bifunctional lycopene cyclase/phytoene synthase		PRO_0000409240
Regions
	Transmembrane	7 – 27	21	Helical; Potential
	Transmembrane	35 – 55	21	Helical; Potential
	Transmembrane	69 – 89	21	Helical; Potential
	Transmembrane	110 – 130	21	Helical; Potential
	Transmembrane	142 – 162	21	Helical; Potential
	Transmembrane	173 – 193	21	Helical; Potential
	Transmembrane	211 – 231	21	Helical; Potential
	Region	1 – 238	238	Lycopene beta-cyclase
	Region	245 – 602	358	Phytoene synthase
Experimental info
	Mutagenesis	77	1	E → K in carR23; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. Ref.1
	Mutagenesis	215	1	P → S in carR21; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. Ref.1
	Mutagenesis	465	1	I → T in carA113; reduces the response to chemical activation. Ref.1
	Mutagenesis	482	1	P → L in carA5; abolishes phytoene synthase activity resulting in a 10- to 15-fold reduction of the beta-carotene content. Ref.1
	Sequence conflict	436	1	A → G in CAB86388. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9P854 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: FF2C7525405D7F99	FASTA	602	68,670
	10 20 30 40 50 60 MLTYMEVHLY FTLPVLALLA FLYKPFFTTK DRFKYIFLCT VAFATASPWD NYIVYHKAWS 70 80 90 100 110 120 YCPECVTAVI GYVPLEEYMF FIIMTLITVT FTSLTMRWTL PSFFIRPETP VFQSVCVRYI 130 140 150 160 170 180 PIVGFLTIAA KAWASSIPDS HPFYGACILW YVCPVLALLW IGSGEYMLRR WKAVLFSIAV 190 200 210 220 230 240 PTIFLCWVDQ YAIARGTWDI SRRTSTGIMV LPSLPLEEFL FFLLIDTVLV FASCATDRAH 250 260 270 280 290 300 AIVHIYITPM NHNKVSTWYM DFFYLCWAFL QTDQALSGET LSDLDATWRI LREASASFYT 310 320 330 340 350 360 ASSVFSFEAR QDLGVLYGFC RATDDLADNN DVSVPDRKKQ LELVRGFVRQ MFDSKHGHPD 370 380 390 400 410 420 IDWTQYSGSI PDSFIAAFRS FTRLRDVLEI KAVEELLDGY TFDLEQREVK NEDDLVYYSA 430 440 450 460 470 480 CVASSVGEMC TRVLMASEPG GNRTMLKWTV ERARDMGLAL QLTNIARDIV TDSKQLGRSY 490 500 510 520 530 540 VPRDWLTSQE SALLKAGKAR ELGDERLRQI ALKMVYTADD LNLMASRAID YLPPSSRCGV 550 560 570 580 590 600 RAACNVYTAI GVSLHKANGY PDRAHLTKLE RMKVTFRCVY GFRKGHQGVQ GDRGKSQAFT VI

References

[1]

"A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces." Arrach N., Fernandez-Martin R., Cerda-Olmedo E., Avalos J. Proc. Natl. Acad. Sci. U.S.A. 98:1687-1692(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-77; PRO-215; ILE-465 AND PRO-482, FUNCTION. Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

[2]

"The carRA gene of Phycomyces blakesleeanus." Sanz C.S., Benito E.P., Eslava A.P. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ278287 Genomic DNA. Translation: CAB93661.1. AJ276965 Genomic DNA. Translation: CAB86388.1.
3D structure databases
ProteinModelPortal	Q9P854.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00799; UER00773. UPA00802.
Family and domain databases
Gene3D	1.10.600.10. 1 hit.
InterPro	IPR017825. Lycopene_cyclase_dom. IPR002060. Squ/phyt_synthse. IPR019845. Squalene/phytoene_synthase_CS. IPR008949. Terpenoid_synth. [Graphical view]
Pfam	PF00494. SQS_PSY. 1 hit. [Graphical view]
SUPFAM	SSF48576. Terpenoid_synth. 1 hit.
TIGRFAMs	TIGR03462. CarR_dom_SF. 2 hits.
PROSITE	PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LCPS_PHYB8

Accession

Primary (citable) accession number: Q9P854
Secondary accession number(s): Q9P877

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 31, 2011
Last sequence update:	October 1, 2000
Last modified:	April 3, 2013
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program