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Q9P854 (LCPS_PHYB8) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 55. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Bifunctional lycopene cyclase/phytoene synthase

Including the following 2 domains:

  1. Lycopene beta-cyclase
    EC=5.5.1.19
    Alternative name(s):
    Lycopene cyclase
  2. Phytoene synthase
    EC=2.5.1.32
Gene names
Name:carRA
OrganismPhycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Taxonomic identifier763407 [NCBI]
Taxonomic lineageEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

Protein attributes

Sequence length602 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase). Ref.1

Catalytic activity

Carotenoid psi-end group = carotenoid beta-end group.

2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

Pathway

Carotenoid biosynthesis; beta-carotene biosynthesis.

Carotenoid biosynthesis; phytoene biosynthesis; all-trans-phytoene from geranylgeranyl diphosphate: step 1/1.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Sequence similarities

In the N-terminal section; belongs to the lycopene beta-cyclase family.

In the C-terminal section; belongs to the phytoene/squalene synthase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 602602Bifunctional lycopene cyclase/phytoene synthase
PRO_0000409240

Regions

Transmembrane7 – 2721Helical; Potential
Transmembrane35 – 5521Helical; Potential
Transmembrane69 – 8921Helical; Potential
Transmembrane110 – 13021Helical; Potential
Transmembrane142 – 16221Helical; Potential
Transmembrane173 – 19321Helical; Potential
Transmembrane211 – 23121Helical; Potential
Region1 – 238238Lycopene beta-cyclase
Region245 – 602358Phytoene synthase

Experimental info

Mutagenesis771E → K in carR23; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. Ref.1
Mutagenesis2151P → S in carR21; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. Ref.1
Mutagenesis4651I → T in carA113; reduces the response to chemical activation. Ref.1
Mutagenesis4821P → L in carA5; abolishes phytoene synthase activity resulting in a 10- to 15-fold reduction of the beta-carotene content. Ref.1
Sequence conflict4361A → G in CAB86388. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Q9P854 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: FF2C7525405D7F99

FASTA60268,670
        10         20         30         40         50         60 
MLTYMEVHLY FTLPVLALLA FLYKPFFTTK DRFKYIFLCT VAFATASPWD NYIVYHKAWS 

        70         80         90        100        110        120 
YCPECVTAVI GYVPLEEYMF FIIMTLITVT FTSLTMRWTL PSFFIRPETP VFQSVCVRYI 

       130        140        150        160        170        180 
PIVGFLTIAA KAWASSIPDS HPFYGACILW YVCPVLALLW IGSGEYMLRR WKAVLFSIAV 

       190        200        210        220        230        240 
PTIFLCWVDQ YAIARGTWDI SRRTSTGIMV LPSLPLEEFL FFLLIDTVLV FASCATDRAH 

       250        260        270        280        290        300 
AIVHIYITPM NHNKVSTWYM DFFYLCWAFL QTDQALSGET LSDLDATWRI LREASASFYT 

       310        320        330        340        350        360 
ASSVFSFEAR QDLGVLYGFC RATDDLADNN DVSVPDRKKQ LELVRGFVRQ MFDSKHGHPD 

       370        380        390        400        410        420 
IDWTQYSGSI PDSFIAAFRS FTRLRDVLEI KAVEELLDGY TFDLEQREVK NEDDLVYYSA 

       430        440        450        460        470        480 
CVASSVGEMC TRVLMASEPG GNRTMLKWTV ERARDMGLAL QLTNIARDIV TDSKQLGRSY 

       490        500        510        520        530        540 
VPRDWLTSQE SALLKAGKAR ELGDERLRQI ALKMVYTADD LNLMASRAID YLPPSSRCGV 

       550        560        570        580        590        600 
RAACNVYTAI GVSLHKANGY PDRAHLTKLE RMKVTFRCVY GFRKGHQGVQ GDRGKSQAFT 


VI 

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References

[1]"A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces."
Arrach N., Fernandez-Martin R., Cerda-Olmedo E., Avalos J.
Proc. Natl. Acad. Sci. U.S.A. 98:1687-1692(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-77; PRO-215; ILE-465 AND PRO-482, FUNCTION.
Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.
[2]"The carRA gene of Phycomyces blakesleeanus."
Sanz C.S., Benito E.P., Eslava A.P.
Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ278287 Genomic DNA. Translation: CAB93661.1.
AJ276965 Genomic DNA. Translation: CAB86388.1.

3D structure databases

ProteinModelPortalQ9P854.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00799; UER00773.
UPA00802.

Family and domain databases

Gene3D1.10.600.10. 1 hit.
InterProIPR017825. Lycopene_cyclase_dom.
IPR002060. Squ/phyt_synthse.
IPR019845. Squalene/phytoene_synthase_CS.
IPR008949. Terpenoid_synth.
[Graphical view]
PfamPF00494. SQS_PSY. 1 hit.
[Graphical view]
SUPFAMSSF48576. SSF48576. 1 hit.
TIGRFAMsTIGR03462. CarR_dom_SF. 2 hits.
PROSITEPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameLCPS_PHYB8
AccessionPrimary (citable) accession number: Q9P854
Secondary accession number(s): Q9P877
Entry history
Integrated into UniProtKB/Swiss-Prot: May 31, 2011
Last sequence update: October 1, 2000
Last modified: June 11, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways