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Q9P854

- LCPS_PHYB8

UniProt

Q9P854 - LCPS_PHYB8

Protein

Bifunctional lycopene cyclase/phytoene synthase

Gene

carRA

Organism
Phycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 56 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Bifunctional enzyme that catalyzes the reactions from geranylgeranyl diphosphate to phytoene (phytoene synthase) and lycopene to beta-carotene via the intermediate gamma-carotene (lycopene cyclase).1 Publication

    Catalytic activityi

    Carotenoid psi-end group = carotenoid beta-end group.
    2 geranylgeranyl diphosphate = 15-cis-phytoene + 2 diphosphate.

    Pathwayi

    GO - Molecular functioni

    1. geranylgeranyl-diphosphate geranylgeranyltransferase activity Source: UniProtKB
    2. intramolecular lyase activity Source: InterPro
    3. lycopene beta cyclase activity Source: UniProtKB
    4. phytoene synthase activity Source: UniProtKB

    GO - Biological processi

    1. carotene biosynthetic process Source: UniProtKB
    2. carotenoid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Isomerase, Transferase

    Keywords - Biological processi

    Carotenoid biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00799; UER00773.
    UPA00802.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bifunctional lycopene cyclase/phytoene synthase
    Including the following 2 domains:
    Lycopene beta-cyclase (EC:5.5.1.19)
    Alternative name(s):
    Lycopene cyclase
    Phytoene synthase (EC:2.5.1.32)
    Gene namesi
    Name:carRA
    OrganismiPhycomyces blakesleeanus (strain ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555)
    Taxonomic identifieri763407 [NCBI]
    Taxonomic lineageiEukaryotaFungiFungi incertae sedisEarly diverging fungal lineagesMucoromycotinaMucoralesPhycomycetaceaePhycomyces

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi77 – 771E → K in carR23; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. 1 Publication
    Mutagenesisi215 – 2151P → S in carR21; abolishes lycopene cyclase activity, rich in lycopene and insensitive to retinol. 1 Publication
    Mutagenesisi465 – 4651I → T in carA113; reduces the response to chemical activation. 1 Publication
    Mutagenesisi482 – 4821P → L in carA5; abolishes phytoene synthase activity resulting in a 10- to 15-fold reduction of the beta-carotene content. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 602602Bifunctional lycopene cyclase/phytoene synthasePRO_0000409240Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P854.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei35 – 5521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei69 – 8921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei110 – 13021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei142 – 16221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei173 – 19321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 238238Lycopene beta-cyclaseAdd
    BLAST
    Regioni245 – 602358Phytoene synthaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the lycopene beta-cyclase family.Curated
    In the C-terminal section; belongs to the phytoene/squalene synthase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.600.10. 1 hit.
    InterProiIPR017825. Lycopene_cyclase_dom.
    IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view]
    PfamiPF00494. SQS_PSY. 1 hit.
    [Graphical view]
    SUPFAMiSSF48576. SSF48576. 1 hit.
    TIGRFAMsiTIGR03462. CarR_dom_SF. 2 hits.
    PROSITEiPS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9P854-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLTYMEVHLY FTLPVLALLA FLYKPFFTTK DRFKYIFLCT VAFATASPWD    50
    NYIVYHKAWS YCPECVTAVI GYVPLEEYMF FIIMTLITVT FTSLTMRWTL 100
    PSFFIRPETP VFQSVCVRYI PIVGFLTIAA KAWASSIPDS HPFYGACILW 150
    YVCPVLALLW IGSGEYMLRR WKAVLFSIAV PTIFLCWVDQ YAIARGTWDI 200
    SRRTSTGIMV LPSLPLEEFL FFLLIDTVLV FASCATDRAH AIVHIYITPM 250
    NHNKVSTWYM DFFYLCWAFL QTDQALSGET LSDLDATWRI LREASASFYT 300
    ASSVFSFEAR QDLGVLYGFC RATDDLADNN DVSVPDRKKQ LELVRGFVRQ 350
    MFDSKHGHPD IDWTQYSGSI PDSFIAAFRS FTRLRDVLEI KAVEELLDGY 400
    TFDLEQREVK NEDDLVYYSA CVASSVGEMC TRVLMASEPG GNRTMLKWTV 450
    ERARDMGLAL QLTNIARDIV TDSKQLGRSY VPRDWLTSQE SALLKAGKAR 500
    ELGDERLRQI ALKMVYTADD LNLMASRAID YLPPSSRCGV RAACNVYTAI 550
    GVSLHKANGY PDRAHLTKLE RMKVTFRCVY GFRKGHQGVQ GDRGKSQAFT 600
    VI 602
    Length:602
    Mass (Da):68,670
    Last modified:October 1, 2000 - v1
    Checksum:iFF2C7525405D7F99
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti436 – 4361A → G in CAB86388. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278287 Genomic DNA. Translation: CAB93661.1.
    AJ276965 Genomic DNA. Translation: CAB86388.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ278287 Genomic DNA. Translation: CAB93661.1 .
    AJ276965 Genomic DNA. Translation: CAB86388.1 .

    3D structure databases

    ProteinModelPortali Q9P854.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00799 ; UER00773 .
    UPA00802 .

    Family and domain databases

    Gene3Di 1.10.600.10. 1 hit.
    InterProi IPR017825. Lycopene_cyclase_dom.
    IPR002060. Squ/phyt_synthse.
    IPR019845. Squalene/phytoene_synthase_CS.
    IPR008949. Terpenoid_synth.
    [Graphical view ]
    Pfami PF00494. SQS_PSY. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48576. SSF48576. 1 hit.
    TIGRFAMsi TIGR03462. CarR_dom_SF. 2 hits.
    PROSITEi PS01045. SQUALEN_PHYTOEN_SYN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A single gene for lycopene cyclase, phytoene synthase, and regulation of carotene biosynthesis in Phycomyces."
      Arrach N., Fernandez-Martin R., Cerda-Olmedo E., Avalos J.
      Proc. Natl. Acad. Sci. U.S.A. 98:1687-1692(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF GLU-77; PRO-215; ILE-465 AND PRO-482, FUNCTION.
      Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.
    2. "The carRA gene of Phycomyces blakesleeanus."
      Sanz C.S., Benito E.P., Eslava A.P.
      Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 8743b / FGSC 10004 / NBRC 33097 / NRRL 1555.

    Entry informationi

    Entry nameiLCPS_PHYB8
    AccessioniPrimary (citable) accession number: Q9P854
    Secondary accession number(s): Q9P877
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 31, 2011
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 56 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3