Reviewed, UniProtKB/Swiss-Prot Q9P804 (TRM1_SCHPO)

Names and origin

Protein names

Recommended name:
    N(2),N(2)-dimethylguanosine tRNA methyltransferase
    EC=2.1.1.32
Alternative name(s):
    tRNA(guanine-26,N(2)-N(2)) methyltransferase
    tRNA 2,2-dimethylguanosine-26 methyltransferase
    tRNA(m(2,2)G26)dimethyltransferase

Gene names

Name:	trm1
ORF Names:	SPBC25D12.05

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(2)-methylguanine.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Note: Nuclear/cytoplasmic compartments By similarity.
Sequence similarities	Belongs to the TRM1 family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm Nucleus
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N2-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 548	548	N(2),N(2)-dimethylguanosine tRNA methyltransferase		PRO_0000147677
Experimental info
	Sequence conflict	322	1	S → T in CAA11801. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9P804-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: E0DD19178A6B4959	FASTA	548	60,297
	10 20 30 40 50 60 MYEANMLRLS QRISNAVNHF STAMMSGASA SLTEGSAIIP FSNPNEVFYN PVQQFNRDLS 70 80 90 100 110 120 VTAIRAWSET RSKKIVAKSH HRHQLLDQNS ELSQENLTKC DTTHDFEKNC SEKTDSTSAD 130 140 150 160 170 180 NIAGFTILEA LSATGLRSIR YAKELPNVKR ILANDLLENA VKTIEKNVNY NNVSDIVIPN 190 200 210 220 230 240 KGDANAVMHM NKFHYDVIDL DPYGSAAPFL DAAVQSVSKD GLLCITCTDS AVLAGNAYPE 250 260 270 280 290 300 KCFSNYGGSS LRSNFCHEQA VRHLLYAIAA SAAKYGRAIK PLLSLSIDFY FRVFVQIKAK 310 320 330 340 350 360 PVLVKNLQSQ SLLIYHCSGC GSFAEQPLGK TSPGRLPGTT KFSNASGPPV SANCEHCGYV 370 380 390 400 410 420 HHVGGPLWGG PLHDAEFLKK MRAIAEDLDP EVYGTKRRIL GMLALADEEL PDVPFYFVLS 430 440 450 460 470 480 QICSVLRSQS PPQNIFVSAL LNAGYRVSGS HAKSNAIKTN APWSFVWDVL RSWIKDHPVK 490 500 510 520 530 540 LENISKTSAG AAILEKTPTA EVDFTFRPDS EFASKKEGYT RYQMNPTENW GPKSKPGKRT IAEVDSKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The tRNA N2,N2-dimethylguanosine-26 methyltransferase encoded by gene trm1 increases efficiency of suppression of an ochre codon in Schizosaccharomyces pombe." Niederberger C., Graub R., Costa A., Desgres J., Schweingruber M.E. FEBS Lett. 464:67-70(1999) [PubMed: 10611485] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AJ224000 Genomic DNA. Translation: CAA11801.1. CU329671 Genomic DNA. Translation: CAA20101.2.
PIR	T39993. T46565.
RefSeq	NP_596547.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	2540604.
KEGG	spo:SPBC25D12.05.
NMPDR	fig|4896.1.peg.2413.
Organism-specific databases
GeneDB_Spombe	SPBC25D12.05.
Phylogenomic databases
OMA	Q9P804. AAMENGT.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-004543-MON.
BRENDA	2.1.1.32. 653.
Gene expression databases
ArrayExpress	Q9P804.
Family and domain databases
InterPro	IPR002905. TRM_MeTrfase. [Graphical view]
PANTHER	PTHR10631. TRM_mtfrase. 1 hit.
Pfam	PF02005. TRM. 1 hit. [Graphical view]
TIGRFAMs	TIGR00308. TRM1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRM1_SCHPO

Accession

Primary (citable) accession number: Q9P804
Secondary accession number(s): O74566

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)