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Protein

tRNA (guanine(26)-N(2))-dimethyltransferase

Gene

trm1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.

Catalytic activityi

2 S-adenosyl-L-methionine + guanine(26) in tRNA = 2 S-adenosyl-L-homocysteine + N(2)-dimethylguanine(26) in tRNA.PROSITE-ProRule annotation

GO - Molecular functioni

  • tRNA (guanine-N2-)-methyltransferase activity Source: PomBase
  • tRNA binding Source: UniProtKB-KW

GO - Biological processi

  • tRNA methylation Source: PomBase
  • tRNA N2-guanine methylation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine, tRNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine(26)-N(2))-dimethyltransferase (EC:2.1.1.216)
Alternative name(s):
tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase
Gene namesi
Name:trm1
ORF Names:SPBC25D12.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:SPBC25D12.05.
PomBaseiSPBC25D12.05. trm1.

Subcellular locationi

  • Nucleus By similarity
  • Cytoplasm By similarity

  • Note: Nuclear/cytoplasmic compartments.By similarity

GO - Cellular componenti

  • mitochondrion Source: PomBase
  • nuclear membrane Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 548548tRNA (guanine(26)-N(2))-dimethyltransferasePRO_0000147677Add
BLAST

Proteomic databases

MaxQBiQ9P804.

Interactioni

Protein-protein interaction databases

BioGridi277130. 7 interactions.
MINTiMINT-4706781.

Structurei

3D structure databases

ProteinModelPortaliQ9P804.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 470441Trm1 methyltransferasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.PROSITE-ProRule annotation
Contains 1 Trm1 methyltransferase domain.PROSITE-ProRule annotation

Phylogenomic databases

HOGENOMiHOG000177995.
InParanoidiQ9P804.
KOiK00555.
OMAiESHANRH.
OrthoDBiEOG78D7VT.
PhylomeDBiQ9P804.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P804-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEANMLRLS QRISNAVNHF STAMMSGASA SLTEGSAIIP FSNPNEVFYN
60 70 80 90 100
PVQQFNRDLS VTAIRAWSET RSKKIVAKSH HRHQLLDQNS ELSQENLTKC
110 120 130 140 150
DTTHDFEKNC SEKTDSTSAD NIAGFTILEA LSATGLRSIR YAKELPNVKR
160 170 180 190 200
ILANDLLENA VKTIEKNVNY NNVSDIVIPN KGDANAVMHM NKFHYDVIDL
210 220 230 240 250
DPYGSAAPFL DAAVQSVSKD GLLCITCTDS AVLAGNAYPE KCFSNYGGSS
260 270 280 290 300
LRSNFCHEQA VRHLLYAIAA SAAKYGRAIK PLLSLSIDFY FRVFVQIKAK
310 320 330 340 350
PVLVKNLQSQ SLLIYHCSGC GSFAEQPLGK TSPGRLPGTT KFSNASGPPV
360 370 380 390 400
SANCEHCGYV HHVGGPLWGG PLHDAEFLKK MRAIAEDLDP EVYGTKRRIL
410 420 430 440 450
GMLALADEEL PDVPFYFVLS QICSVLRSQS PPQNIFVSAL LNAGYRVSGS
460 470 480 490 500
HAKSNAIKTN APWSFVWDVL RSWIKDHPVK LENISKTSAG AAILEKTPTA
510 520 530 540
EVDFTFRPDS EFASKKEGYT RYQMNPTENW GPKSKPGKRT IAEVDSKS
Length:548
Mass (Da):60,297
Last modified:October 1, 2000 - v1
Checksum:iE0DD19178A6B4959
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti322 – 3221S → T in CAA11801 (PubMed:10611485).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224000 Genomic DNA. Translation: CAA11801.1.
CU329671 Genomic DNA. Translation: CAA20101.2.
PIRiT39993.
T46565.
RefSeqiNP_596547.1. NM_001022468.2.

Genome annotation databases

EnsemblFungiiSPBC25D12.05.1; SPBC25D12.05.1:pep; SPBC25D12.05.
GeneIDi2540604.
KEGGispo:SPBC25D12.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224000 Genomic DNA. Translation: CAA11801.1.
CU329671 Genomic DNA. Translation: CAA20101.2.
PIRiT39993.
T46565.
RefSeqiNP_596547.1. NM_001022468.2.

3D structure databases

ProteinModelPortaliQ9P804.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi277130. 7 interactions.
MINTiMINT-4706781.

Proteomic databases

MaxQBiQ9P804.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPBC25D12.05.1; SPBC25D12.05.1:pep; SPBC25D12.05.
GeneIDi2540604.
KEGGispo:SPBC25D12.05.

Organism-specific databases

EuPathDBiFungiDB:SPBC25D12.05.
PomBaseiSPBC25D12.05. trm1.

Phylogenomic databases

HOGENOMiHOG000177995.
InParanoidiQ9P804.
KOiK00555.
OMAiESHANRH.
OrthoDBiEOG78D7VT.
PhylomeDBiQ9P804.

Miscellaneous databases

NextBioi20801729.
PROiQ9P804.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR002905. Trm1.
[Graphical view]
PANTHERiPTHR10631. PTHR10631. 1 hit.
PfamiPF02005. TRM. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 3 hits.
TIGRFAMsiTIGR00308. TRM1. 1 hit.
PROSITEiPS51626. SAM_MT_TRM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The tRNA N2,N2-dimethylguanosine-26 methyltransferase encoded by gene trm1 increases efficiency of suppression of an ochre codon in Schizosaccharomyces pombe."
    Niederberger C., Graub R., Costa A., Desgres J., Schweingruber M.E.
    FEBS Lett. 464:67-70(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiTRM1_SCHPO
AccessioniPrimary (citable) accession number: Q9P804
Secondary accession number(s): O74566
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: October 1, 2000
Last modified: May 11, 2016
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.