Reviewed, UniProtKB/Swiss-Prot Q9P7X8 (RPA2_SCHPO)

Names and origin

Protein names

Recommended name:
    Probable DNA-directed RNA polymerase I subunit RPA2
    EC=2.7.7.6
Alternative name(s):
    DNA-directed RNA polymerase I polypeptide 2
      Short name=RNA polymerase I subunit 2

Gene names

Name:	rpa2
ORF Names:	SPBP23A10.07

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1227 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Second largest core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol I is composed of mobile elements and RPA2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	Component of the RNA polymerase I (Pol I) complex consisting of 14 subunits By similarity.
Subcellular location	Nucleus › nucleolus By similarity.
Sequence similarities	Belongs to the RNA polymerase beta chain family.

Ontologies

Keywords
Biological process	Transcription
Cellular component	DNA-directed RNA polymerase Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	transcription Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	nucleolus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW ribonucleoside binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1227	1227	Probable DNA-directed RNA polymerase I subunit RPA2		PRO_0000048079
Regions
	Zinc finger	1142 – 1171	30	C4-type Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9P7X8-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 614A45ADE74D3419	FASTA	1,227	137,740
	10 20 30 40 50 60 MNNAILLHIP HFSALMFKPF YRFSLKLVKC SLNAQFSMNI AGVLRQNFLC KPGMSFQTLE 70 80 90 100 110 120 RERTFKNPPK DGTSFPDLQK AVKPHVDSFN ALTNAGLLNY AVKEIGEKCA FDSITQEEGG 130 140 150 160 170 180 ALKFGNKISF RVDEVQIAKP MLSSRERSSI NRKVYPAEAR ERLTTYKSRL VLKFSWSVNG 190 200 210 220 230 240 GPRQSEMREV GMIPIMVRSN RCHLEGLSPA ELIAHKEESE EMGGYFIVNG IEKLIRMLIL 250 260 270 280 290 300 PKRNHPTAII RPSFANRGTS YSQYGLSIRC VRPDQSSLTN TLHYLNNGVT MFRFHWRKNE 310 320 330 340 350 360 YLIPSMMILK ALLETSDKEI FEGIVGKDLG NTFLTDRVEL MLRAYKSYGL YSQTETLQYL 370 380 390 400 410 420 GSKFRVVLGV AEDLTDVEVG RFLLQKVVLV HLREAKDKFR LLLFMIRKLY ALVAGECCAD 430 440 450 460 470 480 NPDSPQHQEI LLGGFLYGQI LKEKIEDWLN SIRAQINLDV RRSAPGVDFS DRKYLTRVFS 490 500 510 520 530 540 KINNDIGTKL QYFLSTGNLV SNTGLDLQQA TGYTVVAEKL NFYRFLSHFR MVHRGAFFAE 550 560 570 580 590 600 LKTTTVRKLL PEAWGFMCPV HTPDGSPCGL LNHLARKCEI VTHPSDVSQI PSLLLSLGVD 610 620 630 640 650 660 PPSVVGHESG WGCVQLDGKI VGWCTYKLAK HVADVLRLMK IEYAVKLRNG TATEPAKVPL 670 680 690 700 710 720 DLEIGYVPPS HNGQYPGLYL FSNPARMVRP VKHISTGELD MLGPFEQVYM DIACFPKEIV 730 740 750 760 770 780 PKVSTHVEYS PTNVLSIVAN MTPFSDFNQS PRNMYQCQMG KQTMGTPGTA LRYRTDNKLY 790 800 810 820 830 840 RLQTGQTPVV RPKLHNTYGL DHYPNGTNAV VAVISYTGYD MEDAMILNKS AHERGFGYGT 850 860 870 880 890 900 VYKGESFDLS QKRRRGEPVV HHFGFAPGST PRREWLQKLD ADGLPFIGIK LEDGDPIVAY 910 920 930 940 950 960 YDESTGQNFI ETYHGTEPGF VDEVRLLGND VGDSECQQIH VKLRITRSPI IGDKFSSRHG 970 980 990 1000 1010 1020 QKGICSQKWP TVDMPFTESG MQPDIIINPH AFPSRMTIGM FIESLAGKAG ACHGLAQDST 1030 1040 1050 1060 1070 1080 PFIYSEQQTA ADYFGEQLVK AGYNYHGNEP MYSGITGQEM KADIYIGVVY YQRLRHMVSD 1090 1100 1110 1120 1130 1140 KFQVRTTGPI HNLTRQPVKG RKRAGGIRFG EMERDAVIGH GTSFLMQDRL MNCSDYAQSW 1150 1160 1170 1180 1190 1200 VCRDCGSIIS IMSTISMNGV GSASEVRCRS CAKPALGLED TSDIWQDGSG KKFVGGTNTT 1210 1220 LIALPSVFNY LTAELTAMNI KMMLEVK

References

[1]

"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CU329671 Genomic DNA. Translation: CAB66435.1.
PIR	T50394.
RefSeq	NP_595819.1.
3D structure databases
HSSP	HSSP built from PDB template 1I6H based on UniProtKB P08518.
ModBase	Search...
Genome annotation databases
GeneID	2541280.
KEGG	spo:SPBP23A10.07.
NMPDR	fig|4896.1.peg.1685.
Organism-specific databases
GeneDB_Spombe	SPBP23A10.07.
Phylogenomic databases
OMA	Q9P7X8. CGLMNHM.
Enzyme and pathway databases
BioCyc	SPOM-XXX-01:SPOM-XXX-01-003892-MON.
BRENDA	2.7.7.6. 653.
Gene expression databases
ArrayExpress	Q9P7X8.
Family and domain databases
InterPro	IPR015712. DNA-dir_RNA_pol_su2. IPR007120. DNA-dir_RNA_pol_su2_6. IPR007121. RNA_pol_bsu_CS. IPR007644. RNA_pol_bsu_protrusion. IPR009674. RNA_pol_Rpa2-specific. IPR007642. RNA_pol_Rpb2_2. IPR007645. RNA_pol_Rpb2_3. IPR007647. RNA_pol_Rpb2_5. IPR007641. RNA_pol_Rpb2_7. [Graphical view]
PANTHER	PTHR20856. RNA_pol_I_sub2. 1 hit.
Pfam	PF06883. RNA_pol_Rpa2_4. 1 hit. PF04563. RNA_pol_Rpb2_1. 1 hit. PF04561. RNA_pol_Rpb2_2. 1 hit. PF04565. RNA_pol_Rpb2_3. 1 hit. PF04567. RNA_pol_Rpb2_5. 1 hit. PF00562. RNA_pol_Rpb2_6. 1 hit. PF04560. RNA_pol_Rpb2_7. 1 hit. [Graphical view]
PROSITE	PS01166. RNA_POL_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RPA2_SCHPO

Accession

Primary (citable) accession number: Q9P7X8

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	October 1, 2000
Last modified:	June 16, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)