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Protein

ATP-binding cassette transporter abc4

Gene

abc4

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Involved in detoxification of xenobiotics, and vacuolar sequestration of glutathione S-conjugates. Together with abc2, required for accumulation of a red pigment (ade pigment) in the vacuole of a mutant affected in the adenine biosynthetic pathway.1 Publication

Catalytic activityi

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi648 – 655ATP 1PROSITE-ProRule annotation8
Nucleotide bindingi1246 – 1253ATP 2PROSITE-ProRule annotation8

GO - Molecular functioni

GO - Biological processi

  • glutathione transmembrane import into vacuole Source: PomBase
  • phytochelatin 2 import into vacuole Source: PomBase
  • response to toxic substance Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Detoxification, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-binding cassette transporter abc4 (EC:3.6.3.44)
Short name:
ABC transporter abc4
Alternative name(s):
ATP-energized glutathione S-conjugate pump abc4
Glutathione S-conjugate-transporting ATPase abc4
Gene namesi
Name:abc4Imported
ORF Names:SPAC30.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC30.04c.
PomBaseiSPAC30.04c. abc4.

Subcellular locationi

  • Vacuole membrane Sequence analysis; Multi-pass membrane protein PROSITE-ProRule annotation1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei21 – 40HelicalPROSITE-ProRule annotationAdd BLAST20
Transmembranei55 – 74HelicalPROSITE-ProRule annotationAdd BLAST20
Transmembranei94 – 114HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei121 – 141HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei160 – 180HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei189 – 209HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei296 – 316HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei337 – 357HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei412 – 432HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei441 – 461HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei524 – 544HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei553 – 573HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei894 – 914HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei936 – 956HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1009 – 1029HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1033 – 1053HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1065 – 1085HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1120 – 1140HelicalPROSITE-ProRule annotationAdd BLAST21
Transmembranei1148 – 1168HelicalPROSITE-ProRule annotationAdd BLAST21

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • vacuolar membrane Source: UniProtKB-SubCell
  • vacuole Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

Pathology & Biotechi

Disruption phenotypei

Cells lacking both abc2 and abc4 show sensitivity to cycloheximide (CHX) and 4-nitroquinoline oxide (4-NQO), and decreased accumulation of monochlorobimane-glutathione (MCIB-GS).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003312231 – 1469ATP-binding cassette transporter abc4Add BLAST1469

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi386N-linked (GlcNAc...)Sequence analysis1
Glycosylationi510N-linked (GlcNAc...)Sequence analysis1
Glycosylationi615N-linked (GlcNAc...)Sequence analysis1
Glycosylationi691N-linked (GlcNAc...)Sequence analysis1
Glycosylationi790N-linked (GlcNAc...)Sequence analysis1
Glycosylationi815N-linked (GlcNAc...)Sequence analysis1
Glycosylationi923N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1007N-linked (GlcNAc...)Sequence analysis1
Glycosylationi1355N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiQ9P7V2.
PRIDEiQ9P7V2.

Interactioni

Protein-protein interaction databases

BioGridi279493. 8 interactors.

Structurei

3D structure databases

ProteinModelPortaliQ9P7V2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini296 – 580ABC transmembrane type-1 1PROSITE-ProRule annotationAdd BLAST285
Domaini611 – 840ABC transporter 1PROSITE-ProRule annotationAdd BLAST230
Domaini897 – 1176ABC transmembrane type-1 2PROSITE-ProRule annotationAdd BLAST280
Domaini1214 – 1453ABC transporter 2PROSITE-ProRule annotationAdd BLAST240

Sequence similaritiesi

Contains 2 ABC transmembrane type-1 domains.PROSITE-ProRule annotation
Contains 2 ABC transporter domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000033753.
InParanoidiQ9P7V2.
OMAiFIRIDAL.
OrthoDBiEOG092C0VET.
PhylomeDBiQ9P7V2.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P7V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENFHHRPFK GGFGVGRVPT SLYYSLSDFS LSAISIFPTH YDQPYLNEAP
60 70 80 90 100
SWYKYSLESG LVCLYLYLIY RWITRSFTVK QTVGSEFSGL RKAHGILLWL
110 120 130 140 150
LSILFLGMTA FMFVNDAFPT AFSDPPVKIC LLAYSVHLFL LHSLRMIYPR
160 170 180 190 200
ARPTLYHAAV LLNLLLLPPI FHFYSYPFFF VDHPPLSSYS PFLWFYFFIT
210 220 230 240 250
IVGNFIPLFT PRVWYPLFPE DNYQPSAEQV CSMFNFACSY GYLNPLVLTA
260 270 280 290 300
KRRVVEVDDT PVIPDYDKNK AWTYRFERMK TPSLFITIHK IFWRQILGMG
310 320 330 340 350
VSSFMVSVCQ FLSPIALNRL LKHLESPSSS SVNPLVWIVL LLTGPFLTSL
360 370 380 390 400
FTQFYLFMST RYLARSYTTL VQQIYNKVIR SRFVQNKSGD SKVGRSNNLI
410 420 430 440 450
STDVDDIGEI REFIHIIVRA PVEIAGSIYL LQKLLGWSAY VGLALTVLTC
460 470 480 490 500
SVPIVLGPLV AKLTLRANRA TDSRIELMSE LLQSIRITKF FGWELPMLDR
510 520 530 540 550
VKQRRQVELN RTWKLLLMEI CIQVLVESLP VFSMFATFVV FTTIMGQTIT
560 570 580 590 600
PSIAFTSISL FSFIRTQFSW IAYLMRQIVQ IFVSIGRVSD FLNDPDEVDP
610 620 630 640 650
VNTIEDTSQE IGFFNASLTW VSNPSPGDFC LRDLNIVFPR NKLSIVIGPT
660 670 680 690 700
GSGKSSLISA LLGELSLNKG SYNLPRSKGV SYVSQVPWLR NATIRDNILF
710 720 730 740 750
DYPYIEERYK KVIQACGLLT DLQSFVASDL TEIGEKGVTL SGGQKQRIAL
760 770 780 790 800
ARAVYSPTSI VLMDDVFSAL DIHTSNWIYK HCFQSSLMEN RTVILVTHNV
810 820 830 840 850
HLFMDSAAFI VTVKNGSAFP VTDKSSPLLF LAEQAASASE AAAPDLAAIP
860 870 880 890 900
IPNEVDDAEA ADEKDGKLVE EEQRVTGDVV FSEIFSYMKH FGSGFYVAAV
910 920 930 940 950
LLFFVTTQAT SILIDLWVAF WTNSSVNSPD VNNNKFLFVY GTMLLAYSLL
960 970 980 990 1000
DFLRTVSYDR GAWWASRKLH DSMLESVFGT FASWFDKTPT GRIVNRFAKD
1010 1020 1030 1040 1050
IRSIDMNLSG WLFFSINCFL SVAGGILSVS SAMPIFMIPA VIVCLAGYYF
1060 1070 1080 1090 1100
GLLYTRAQVG VKRLISIYTS PIFSLLGESI VGVSVIRAFN RQTIFKQMFS
1110 1120 1130 1140 1150
ERLDNLVRLQ STSYNLNRWV AVRTDGISGL VGAIAGLIAL LQKDLSPGVV
1160 1170 1180 1190 1200
GFSLNQAVIF SSSVLLFVRS CNSLQAEMNS YERVLEYSKL PQEPAPTIAG
1210 1220 1230 1240 1250
QVPATWPKEG DIVFNHVSVS YSAAGPTILK DVNLHINPSE KVAVVGRTGS
1260 1270 1280 1290 1300
GKSTLGLTLL RFTHRISGEI YINGRETQSV NLNALRQRIS FIPQDPILFS
1310 1320 1330 1340 1350
GTVRSNLDPF DELEDNFLNE ALKTSGASSM IMAHTDDQKP IHITLDTHVA
1360 1370 1380 1390 1400
SEGSNFSQGQ KQVLALARAI VRRSKIIILD ECTASVDDAM DQKIQKTLRE
1410 1420 1430 1440 1450
AFGDATMLCI AHRLKTIVDY DKVMVLDKGV LVEYGPPAVL YHNNGHFRRM
1460
CDGSGITFLP PETRGSKSA
Length:1,469
Mass (Da):164,790
Last modified:October 1, 2000 - v1
Checksum:iEE30BE28F5B9CBDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB66463.1.
PIRiT50210.
RefSeqiNP_594558.1. NM_001019987.2.

Genome annotation databases

EnsemblFungiiSPAC30.04c.1; SPAC30.04c.1:pep; SPAC30.04c.
GeneIDi2543059.
KEGGispo:SPAC30.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB66463.1.
PIRiT50210.
RefSeqiNP_594558.1. NM_001019987.2.

3D structure databases

ProteinModelPortaliQ9P7V2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279493. 8 interactors.

Proteomic databases

MaxQBiQ9P7V2.
PRIDEiQ9P7V2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC30.04c.1; SPAC30.04c.1:pep; SPAC30.04c.
GeneIDi2543059.
KEGGispo:SPAC30.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC30.04c.
PomBaseiSPAC30.04c. abc4.

Phylogenomic databases

HOGENOMiHOG000033753.
InParanoidiQ9P7V2.
OMAiFIRIDAL.
OrthoDBiEOG092C0VET.
PhylomeDBiQ9P7V2.

Miscellaneous databases

PROiQ9P7V2.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR003593. AAA+_ATPase.
IPR011527. ABC1_TM_dom.
IPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR027417. P-loop_NTPase.
[Graphical view]
PfamiPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTiSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 2 hits.
SSF90123. SSF90123. 2 hits.
PROSITEiPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiABC4_SCHPO
AccessioniPrimary (citable) accession number: Q9P7V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2000
Last modified: October 5, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.