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Q9P7V2 (ABC4_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-binding cassette transporter abc4
Short name=ABC transporter abc4
EC=3.6.3.44
Alternative name(s):
ATP-energized glutathione S-conjugate pump abc4
Glutathione S-conjugate-transporting ATPase abc4
Gene names
Name:abc4
ORF Names:SPAC30.04c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length1469 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in detoxification of xenobiotics, and vacuolar sequestration of glutathione S-conjugates. Together with abc2, required for accumulation of a red pigment (ade pigment) in the vacuole of a mutant affected in the adenine biosynthetic pathway. Ref.2

Catalytic activity

ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out). UniProtKB Q9C8G9

Subcellular location

Vacuole membrane; Multi-pass membrane protein Potential Ref.2.

Disruption phenotype

Cells lacking both abc2 and abc4 show sensitivity to cycloheximide (CHX) and 4-nitroquinoline oxide (4-NQO), and decreased accumulation of monochlorobimane-glutathione (MCIB-GS). Ref.2

Sequence similarities

Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification]

Contains 2 ABC transmembrane type-1 domains.

Contains 2 ABC transporter domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 14691469ATP-binding cassette transporter abc4
PRO_0000331223

Regions

Transmembrane21 – 4020Helical; Potential
Transmembrane55 – 7420Helical; Potential
Transmembrane94 – 11421Helical; Potential
Transmembrane121 – 14121Helical; Potential
Transmembrane160 – 18021Helical; Potential
Transmembrane189 – 20921Helical; Potential
Transmembrane296 – 31621Helical; Potential
Transmembrane337 – 35721Helical; Potential
Transmembrane412 – 43221Helical; Potential
Transmembrane441 – 46121Helical; Potential
Transmembrane524 – 54421Helical; Potential
Transmembrane553 – 57321Helical; Potential
Transmembrane894 – 91421Helical; Potential
Transmembrane936 – 95621Helical; Potential
Transmembrane1009 – 102921Helical; Potential
Transmembrane1033 – 105321Helical; Potential
Transmembrane1065 – 108521Helical; Potential
Transmembrane1120 – 114021Helical; Potential
Transmembrane1148 – 116821Helical; Potential
Domain296 – 580285ABC transmembrane type-1 1
Domain611 – 840230ABC transporter 1
Domain897 – 1176280ABC transmembrane type-1 2
Domain1214 – 1453240ABC transporter 2
Nucleotide binding648 – 6558ATP 1 Potential
Nucleotide binding1246 – 12538ATP 2 Potential

Amino acid modifications

Glycosylation3861N-linked (GlcNAc...) Potential
Glycosylation5101N-linked (GlcNAc...) Potential
Glycosylation6151N-linked (GlcNAc...) Potential
Glycosylation6911N-linked (GlcNAc...) Potential
Glycosylation7901N-linked (GlcNAc...) Potential
Glycosylation8151N-linked (GlcNAc...) Potential
Glycosylation9231N-linked (GlcNAc...) Potential
Glycosylation10071N-linked (GlcNAc...) Potential
Glycosylation13551N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
Q9P7V2 [UniParc].

Last modified October 1, 2000. Version 1.
Checksum: EE30BE28F5B9CBDA

FASTA1,469164,790
        10         20         30         40         50         60 
MENFHHRPFK GGFGVGRVPT SLYYSLSDFS LSAISIFPTH YDQPYLNEAP SWYKYSLESG 

        70         80         90        100        110        120 
LVCLYLYLIY RWITRSFTVK QTVGSEFSGL RKAHGILLWL LSILFLGMTA FMFVNDAFPT 

       130        140        150        160        170        180 
AFSDPPVKIC LLAYSVHLFL LHSLRMIYPR ARPTLYHAAV LLNLLLLPPI FHFYSYPFFF 

       190        200        210        220        230        240 
VDHPPLSSYS PFLWFYFFIT IVGNFIPLFT PRVWYPLFPE DNYQPSAEQV CSMFNFACSY 

       250        260        270        280        290        300 
GYLNPLVLTA KRRVVEVDDT PVIPDYDKNK AWTYRFERMK TPSLFITIHK IFWRQILGMG 

       310        320        330        340        350        360 
VSSFMVSVCQ FLSPIALNRL LKHLESPSSS SVNPLVWIVL LLTGPFLTSL FTQFYLFMST 

       370        380        390        400        410        420 
RYLARSYTTL VQQIYNKVIR SRFVQNKSGD SKVGRSNNLI STDVDDIGEI REFIHIIVRA 

       430        440        450        460        470        480 
PVEIAGSIYL LQKLLGWSAY VGLALTVLTC SVPIVLGPLV AKLTLRANRA TDSRIELMSE 

       490        500        510        520        530        540 
LLQSIRITKF FGWELPMLDR VKQRRQVELN RTWKLLLMEI CIQVLVESLP VFSMFATFVV 

       550        560        570        580        590        600 
FTTIMGQTIT PSIAFTSISL FSFIRTQFSW IAYLMRQIVQ IFVSIGRVSD FLNDPDEVDP 

       610        620        630        640        650        660 
VNTIEDTSQE IGFFNASLTW VSNPSPGDFC LRDLNIVFPR NKLSIVIGPT GSGKSSLISA 

       670        680        690        700        710        720 
LLGELSLNKG SYNLPRSKGV SYVSQVPWLR NATIRDNILF DYPYIEERYK KVIQACGLLT 

       730        740        750        760        770        780 
DLQSFVASDL TEIGEKGVTL SGGQKQRIAL ARAVYSPTSI VLMDDVFSAL DIHTSNWIYK 

       790        800        810        820        830        840 
HCFQSSLMEN RTVILVTHNV HLFMDSAAFI VTVKNGSAFP VTDKSSPLLF LAEQAASASE 

       850        860        870        880        890        900 
AAAPDLAAIP IPNEVDDAEA ADEKDGKLVE EEQRVTGDVV FSEIFSYMKH FGSGFYVAAV 

       910        920        930        940        950        960 
LLFFVTTQAT SILIDLWVAF WTNSSVNSPD VNNNKFLFVY GTMLLAYSLL DFLRTVSYDR 

       970        980        990       1000       1010       1020 
GAWWASRKLH DSMLESVFGT FASWFDKTPT GRIVNRFAKD IRSIDMNLSG WLFFSINCFL 

      1030       1040       1050       1060       1070       1080 
SVAGGILSVS SAMPIFMIPA VIVCLAGYYF GLLYTRAQVG VKRLISIYTS PIFSLLGESI 

      1090       1100       1110       1120       1130       1140 
VGVSVIRAFN RQTIFKQMFS ERLDNLVRLQ STSYNLNRWV AVRTDGISGL VGAIAGLIAL 

      1150       1160       1170       1180       1190       1200 
LQKDLSPGVV GFSLNQAVIF SSSVLLFVRS CNSLQAEMNS YERVLEYSKL PQEPAPTIAG 

      1210       1220       1230       1240       1250       1260 
QVPATWPKEG DIVFNHVSVS YSAAGPTILK DVNLHINPSE KVAVVGRTGS GKSTLGLTLL 

      1270       1280       1290       1300       1310       1320 
RFTHRISGEI YINGRETQSV NLNALRQRIS FIPQDPILFS GTVRSNLDPF DELEDNFLNE 

      1330       1340       1350       1360       1370       1380 
ALKTSGASSM IMAHTDDQKP IHITLDTHVA SEGSNFSQGQ KQVLALARAI VRRSKIIILD 

      1390       1400       1410       1420       1430       1440 
ECTASVDDAM DQKIQKTLRE AFGDATMLCI AHRLKTIVDY DKVMVLDKGV LVEYGPPAVL 

      1450       1460 
YHNNGHFRRM CDGSGITFLP PETRGSKSA

« Hide

References

« Hide 'large scale' references
[1]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed: 11859360] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[2]"A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant."
Iwaki T., Giga-Hama Y., Takegawa K.
Microbiology 152:2309-2321(2006) [PubMed: 16849797] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CU329670 Genomic DNA. Translation: CAB66463.1.
PIRT50210.
RefSeqNP_594558.1. NM_001019987.1.

3D structure databases

HSSPHSSP built from PDB template 2BBS based on UniProtKB P13569.
ProteinModelPortalQ9P7V2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC30.04c.1; SPAC30.04c.1:pep; SPAC30.04c.
GeneID2543059.
GenomeReviewsGene locus abc4 in contig CU329670_GR.
KEGGspo:SPAC30.04c.
NMPDRfig|4896.1.peg.4528.

Organism-specific databases

GeneDB_SpombeSPAC30.04c.

Phylogenomic databases

eggNOGfuNOG05955.
GeneTreeEFGT00050000001686.
HOGENOMHBG206983.
OMAREDELAW.
OrthoDBEOG49CTGR.

Gene expression databases

ArrayExpressQ9P7V2.

Family and domain databases

InterProIPR003439. ABC_transporter-like.
IPR017871. ABC_transporter_CS.
IPR017940. ABC_transporter_type1.
IPR001140. ABC_transptr_TM_dom.
IPR011527. ABC_transptrTM_dom_typ1.
IPR003593. ATPase_AAA+_core.
[Graphical view]
PfamPF00664. ABC_membrane. 2 hits.
PF00005. ABC_tran. 2 hits.
[Graphical view]
SMARTSM00382. AAA. 2 hits.
[Graphical view]
SUPFAMSSF90123. ABC_TM_1. 2 hits.
PROSITEPS50929. ABC_TM1F. 2 hits.
PS00211. ABC_TRANSPORTER_1. 2 hits.
PS50893. ABC_TRANSPORTER_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameABC4_SCHPO
AccessionPrimary (citable) accession number: Q9P7V2
Entry history
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: October 1, 2000
Last modified: December 14, 2011
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents