Reviewed, UniProtKB/Swiss-Prot Q9P7V2 (ABC4_SCHPO)

Names and origin

Protein names

Recommended name:
    ATP-binding cassette transporter abc4
      Short name=ABC transporter abc4
    EC=3.6.3.44
Alternative name(s):
    Glutathione S-conjugate-transporting ATPase abc4
    ATP-energized glutathione S-conjugate pump abc4

Gene names

Name:	abc4
ORF Names:	SPAC30.04c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1469 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Involved in detoxification of xenobiotics, and vacuolar sequestration of glutathione S-conjugates. Together with abc2, required for accumulation of a red pigment (ade pigment) in the vacuole of a mutant affected in the adenine biosynthetic pathway. Ref.2
Catalytic activity	ATP + H2O + xenobiotic(In) = ADP + phosphate + xenobiotic(Out). UniProtKB Q9C8G9
Subcellular location	Vacuole membrane; Multi-pass membrane protein Potential Ref.2.
Disruption phenotype	Cells lacking both abc2 and abc4 show sensitivity to cycloheximide (CHX) and 4-nitroquinoline oxide (4-NQO), and decreased accumulation of monochlorobimane-glutathione (MCIB-GS). Ref.2
Sequence similarities	Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. [View classification] Contains 2 ABC transmembrane type-1 domains. Contains 2 ABC transporter domains.

Ontologies

Keywords
Biological process	Detoxification Transport
Cellular component	Membrane Vacuole
Domain	Repeat Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	response to toxin Inferred from electronic annotation. Source: UniProtKB-KW transmembrane transport Inferred from electronic annotation. Source: InterPro vacuolar transport Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	fungal-type vacuole membrane Ref.2 Inferred from direct assay. Source: GeneDB_SPombe integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred by curator. Source: GeneDB_SPombe glutathione S-conjugate-exporting ATPase activity Ref.2 Inferred from mutant phenotype. Source: GeneDB_SPombe xenobiotic-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1469	1469	ATP-binding cassette transporter abc4		PRO_0000331223
Regions
	Transmembrane	21 – 40	20	Potential
	Transmembrane	55 – 74	20	Potential
	Transmembrane	94 – 114	21	Potential
	Transmembrane	121 – 141	21	Potential
	Transmembrane	160 – 180	21	Potential
	Transmembrane	189 – 209	21	Potential
	Transmembrane	296 – 316	21	Potential
	Transmembrane	337 – 357	21	Potential
	Transmembrane	412 – 432	21	Potential
	Transmembrane	441 – 461	21	Potential
	Transmembrane	524 – 544	21	Potential
	Transmembrane	553 – 573	21	Potential
	Transmembrane	894 – 914	21	Potential
	Transmembrane	936 – 956	21	Potential
	Transmembrane	1009 – 1029	21	Potential
	Transmembrane	1033 – 1053	21	Potential
	Transmembrane	1065 – 1085	21	Potential
	Transmembrane	1120 – 1140	21	Potential
	Transmembrane	1148 – 1168	21	Potential
	Domain	296 – 580	285	ABC transmembrane type-1 1
	Domain	611 – 840	230	ABC transporter 1
	Domain	897 – 1176	280	ABC transmembrane type-1 2
	Domain	1214 – 1453	240	ABC transporter 2
	Nucleotide binding	648 – 655	8	ATP 1 Potential
	Nucleotide binding	1246 – 1253	8	ATP 2 Potential
Amino acid modifications
	Glycosylation	386	1	N-linked (GlcNAc...) Potential
	Glycosylation	510	1	N-linked (GlcNAc...) Potential
	Glycosylation	615	1	N-linked (GlcNAc...) Potential
	Glycosylation	691	1	N-linked (GlcNAc...) Potential
	Glycosylation	790	1	N-linked (GlcNAc...) Potential
	Glycosylation	815	1	N-linked (GlcNAc...) Potential
	Glycosylation	923	1	N-linked (GlcNAc...) Potential
	Glycosylation	1007	1	N-linked (GlcNAc...) Potential
	Glycosylation	1355	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9P7V2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: EE30BE28F5B9CBDA	FASTA	1,469	164,790
	10 20 30 40 50 60 MENFHHRPFK GGFGVGRVPT SLYYSLSDFS LSAISIFPTH YDQPYLNEAP SWYKYSLESG 70 80 90 100 110 120 LVCLYLYLIY RWITRSFTVK QTVGSEFSGL RKAHGILLWL LSILFLGMTA FMFVNDAFPT 130 140 150 160 170 180 AFSDPPVKIC LLAYSVHLFL LHSLRMIYPR ARPTLYHAAV LLNLLLLPPI FHFYSYPFFF 190 200 210 220 230 240 VDHPPLSSYS PFLWFYFFIT IVGNFIPLFT PRVWYPLFPE DNYQPSAEQV CSMFNFACSY 250 260 270 280 290 300 GYLNPLVLTA KRRVVEVDDT PVIPDYDKNK AWTYRFERMK TPSLFITIHK IFWRQILGMG 310 320 330 340 350 360 VSSFMVSVCQ FLSPIALNRL LKHLESPSSS SVNPLVWIVL LLTGPFLTSL FTQFYLFMST 370 380 390 400 410 420 RYLARSYTTL VQQIYNKVIR SRFVQNKSGD SKVGRSNNLI STDVDDIGEI REFIHIIVRA 430 440 450 460 470 480 PVEIAGSIYL LQKLLGWSAY VGLALTVLTC SVPIVLGPLV AKLTLRANRA TDSRIELMSE 490 500 510 520 530 540 LLQSIRITKF FGWELPMLDR VKQRRQVELN RTWKLLLMEI CIQVLVESLP VFSMFATFVV 550 560 570 580 590 600 FTTIMGQTIT PSIAFTSISL FSFIRTQFSW IAYLMRQIVQ IFVSIGRVSD FLNDPDEVDP 610 620 630 640 650 660 VNTIEDTSQE IGFFNASLTW VSNPSPGDFC LRDLNIVFPR NKLSIVIGPT GSGKSSLISA 670 680 690 700 710 720 LLGELSLNKG SYNLPRSKGV SYVSQVPWLR NATIRDNILF DYPYIEERYK KVIQACGLLT 730 740 750 760 770 780 DLQSFVASDL TEIGEKGVTL SGGQKQRIAL ARAVYSPTSI VLMDDVFSAL DIHTSNWIYK 790 800 810 820 830 840 HCFQSSLMEN RTVILVTHNV HLFMDSAAFI VTVKNGSAFP VTDKSSPLLF LAEQAASASE 850 860 870 880 890 900 AAAPDLAAIP IPNEVDDAEA ADEKDGKLVE EEQRVTGDVV FSEIFSYMKH FGSGFYVAAV 910 920 930 940 950 960 LLFFVTTQAT SILIDLWVAF WTNSSVNSPD VNNNKFLFVY GTMLLAYSLL DFLRTVSYDR 970 980 990 1000 1010 1020 GAWWASRKLH DSMLESVFGT FASWFDKTPT GRIVNRFAKD IRSIDMNLSG WLFFSINCFL 1030 1040 1050 1060 1070 1080 SVAGGILSVS SAMPIFMIPA VIVCLAGYYF GLLYTRAQVG VKRLISIYTS PIFSLLGESI 1090 1100 1110 1120 1130 1140 VGVSVIRAFN RQTIFKQMFS ERLDNLVRLQ STSYNLNRWV AVRTDGISGL VGAIAGLIAL 1150 1160 1170 1180 1190 1200 LQKDLSPGVV GFSLNQAVIF SSSVLLFVRS CNSLQAEMNS YERVLEYSKL PQEPAPTIAG 1210 1220 1230 1240 1250 1260 QVPATWPKEG DIVFNHVSVS YSAAGPTILK DVNLHINPSE KVAVVGRTGS GKSTLGLTLL 1270 1280 1290 1300 1310 1320 RFTHRISGEI YINGRETQSV NLNALRQRIS FIPQDPILFS GTVRSNLDPF DELEDNFLNE 1330 1340 1350 1360 1370 1380 ALKTSGASSM IMAHTDDQKP IHITLDTHVA SEGSNFSQGQ KQVLALARAI VRRSKIIILD 1390 1400 1410 1420 1430 1440 ECTASVDDAM DQKIQKTLRE AFGDATMLCI AHRLKTIVDY DKVMVLDKGV LVEYGPPAVL 1450 1460 YHNNGHFRRM CDGSGITFLP PETRGSKSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[2]	"A survey of all 11 ABC transporters in fission yeast: two novel ABC transporters are required for red pigment accumulation in a Schizosaccharomyces pombe adenine biosynthetic mutant." Iwaki T., Giga-Hama Y., Takegawa K. Microbiology 152:2309-2321(2006) [PubMed: 16849797] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329670 Genomic DNA. Translation: CAB66463.1.
PIR	T50210.
RefSeq	NP_594558.1.
3D structure databases
HSSP	HSSP built from PDB template 2BBS based on UniProtKB P13569.
SMR	Q9P7V2. Positions 289-818.
ModBase	Search...
Genome annotation databases
GeneID	2543059.
GenomeReviews	Gene locus abc4 in contig CU329670_GR.
KEGG	spo:SPAC30.04c.
NMPDR	fig|4896.1.peg.4528.
Organism-specific databases
GeneDB_Spombe	SPAC30.04c.
Phylogenomic databases
eggNOG	fuNOG05955.
HOGENOM	HBG206983.
OMA	ADENNEN.
OrthoDB	EOG9P2RK1.
PhylomeDB	Q9P7V2.
Gene expression databases
ArrayExpress	Q9P7V2.
Family and domain databases
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR017940. ABC_transporter_type1. IPR001140. ABC_transptr_TM_dom. IPR011527. ABC_transptrTM_dom_typ1. IPR003593. ATPase_AAA+_core. [Graphical view]
Pfam	PF00664. ABC_membrane. 2 hits. PF00005. ABC_tran. 2 hits. [Graphical view]
SMART	SM00382. AAA. 2 hits. [Graphical view]
PROSITE	PS50929. ABC_TM1F. 2 hits. PS00211. ABC_TRANSPORTER_1. 2 hits. PS50893. ABC_TRANSPORTER_2. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABC4_SCHPO

Accession

Primary (citable) accession number: Q9P7V2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)