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Protein

Mediator of replication checkpoint protein 1

Gene

mrc1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the replisome and is required for rad3-dependent activation of the checkpoint kinase cds1 in response to replication fork arrest. Phosphorylation allows it to mediate the activation of cds1.1 Publication

GO - Molecular functioni

  • DNA binding Source: PomBase
  • protein serine/threonine kinase activator activity Source: PomBase
  • Y-form DNA binding Source: PomBase

GO - Biological processi

  • DNA replication Source: UniProtKB-KW
  • intra-S DNA damage checkpoint Source: PomBase
  • mitotic DNA replication checkpoint Source: PomBase
  • regulation of mitotic DNA replication initiation Source: PomBase
  • response to intra-S DNA damage checkpoint signaling Source: PomBase
  • signal transduction involved in intra-S DNA damage checkpoint Source: PomBase

Keywordsi

Biological processDNA replication

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of replication checkpoint protein 1
Alternative name(s):
DNA replication checkpoint mediator mrc1
Gene namesi
Name:mrc1
ORF Names:SPAC694.06c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC694.06c.
PomBaseiSPAC694.06c. mrc1.

Subcellular locationi

  • Nucleus 1 Publication

  • Note: Associated with chromatin.

GO - Cellular componenti

  • nuclear chromatin Source: PomBase
  • nuclear replication fork Source: PomBase
  • nucleus Source: PomBase
  • protein complex Source: PomBase

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi599S → A: Severe hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi604S → A: Severe hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi614S → A: Severe hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi634T → A: Hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi637S → A: Hydroxyurea (HU) sensitivity. 1 Publication1
Mutagenesisi645T → A: Hydroxyurea (HU) sensitivity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000965731 – 1019Mediator of replication checkpoint protein 1Add BLAST1019

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei604Phosphoserine1 Publication1
Modified residuei634Phosphothreonine1 Publication1
Modified residuei637Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylated by rad3 and tel1.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P7T4.
PRIDEiQ9P7T4.

PTM databases

iPTMnetiQ9P7T4.

Interactioni

Subunit structurei

Interacts with cds1.1 Publication

Protein-protein interaction databases

BioGridi279668. 120 interactors.
ELMiQ9P7T4.
IntActiQ9P7T4. 1 interactor.
MINTiMINT-1177572.
STRINGi4896.SPAC694.06c.1.

Family & Domainsi

Phylogenomic databases

InParanoidiQ9P7T4.
KOiK11272.
OMAiYQIKDQK.
OrthoDBiEOG092C303J.
PhylomeDBiQ9P7T4.

Family and domain databases

InterProiView protein in InterPro
IPR024146. Claspin.
IPR018564. Repl_chkpnt_MRC1_dom.
PANTHERiPTHR14396. PTHR14396. 1 hit.
PfamiView protein in Pfam
PF09444. MRC1. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P7T4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASLDENADE LHRMDSSDEA SINDDQEDIL DTPRTRVRKM LASVDMQLSS
60 70 80 90 100
NAVSEASLDK ESTVGNLENQ KNRSYSSEIY LHSDTNFLSN FDSAYERVRR
110 120 130 140 150
LLNQQGGKSS LQKKEVEQIE TQEGGDNAKG SPSSENKDSD RNSRLQQLIE
160 170 180 190 200
KKRNALKKEQ EDLIQNSATS HSKSDNLDSE SADDSDLADE SELSKKYTSD
210 220 230 240 250
RKIRNASKKA LLELHRNTAR LTRETALKPE VVVKKKVTLR EFFQKIGFKN
260 270 280 290 300
DNQLENKAIS EEEANSTEPP NVEKEEPKPS VDRSTGIVNS EDIKELSVED
310 320 330 340 350
DSLELKEITP EALDIGQTSL FTTLNQTQVK KEDNKKFLLK EINAKLNEDD
360 370 380 390 400
IDSELEIEVK PKTTALDNIE KSKLSEENEH GIKGKLKQLA EIKLSKDGKP
410 420 430 440 450
FENEFNIKSF NRNLVKRAAV MAKLQRNQLE EELKAKGIYK PTIQGEKEEE
460 470 480 490 500
EDPLERARND AEKIRQLEKA SGNASDEGEL NDEEEVISSS NTPSTKAKTT
510 520 530 540 550
NKVIISDVII EATQAEPKRR QKNSRVVFDE EDLTGDSHGS SNMKISESDD
560 570 580 590 600
ESNGDMIRDS FDRLSSESIK DSQKTEELHD SFGINDEVDQ STSLYVQNSQ
610 620 630 640 650
PSASQLTIVD ATYSQPPPRW ESSSRDDKTN TSSTQPSQVD SLVPTQLDST
660 670 680 690 700
IPTQIDSVQR NKDQDDEEIL EERRESRRDS KTFLSRTMLY NKDTGKADSA
710 720 730 740 750
WASDLIEEQA IESDDEYAGI GGLSDDGLSD SDAELEVQNM IDDETTIQKG
760 770 780 790 800
EVASMAQFAK DQEMDRDEKL VKQLMKDVTT GALRKRNRNG FAALDDSDDE
810 820 830 840 850
DYSNLRREKL KELRRQKLLE DGNLNVLEGD KRKAFLATVE DSLVSSKDNL
860 870 880 890 900
TWLDATVEDS GVGSSDLGDE YLYSEQSLNH EEEEQMEEEL SEIFSSGGPN
910 920 930 940 950
VVDRVYLKKS STRHTSDNNS LEEVLPIFPG VRKLVSNSQS EKIGDLSNDN
960 970 980 990 1000
SMGAKSYKTP IISSTQRPQG RKFRGLMNQS SKADISRTVD AGSIKVVPNS
1010
QSANPPRLLA SLNNYSDFD
Length:1,019
Mass (Da):114,319
Last modified:October 1, 2000 - v1
Checksum:i6B5E7A4B5ABE5380
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB71844.1.
PIRiT50251.
RefSeqiNP_594486.1. NM_001019915.2.

Genome annotation databases

EnsemblFungiiSPAC694.06c.1; SPAC694.06c.1:pep; SPAC694.06c.
GeneIDi2543240.
KEGGispo:SPAC694.06c.

Similar proteinsi

Entry informationi

Entry nameiMRC1_SCHPO
AccessioniPrimary (citable) accession number: Q9P7T4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 13, 2004
Last sequence update: October 1, 2000
Last modified: August 30, 2017
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names