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Protein

Alpha-1,2-mannosyltransferase alg9

Gene

alg9

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of mannose from Dol-P-Man to lipid-linked oligosaccharides.By similarity

Catalytic activityi

Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.
Dolichyl beta-D-mannosyl phosphate + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol = D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-[D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->2)-D-Man-alpha-(1->6))-D-Man-alpha-(1->6)]-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol + dolichyl phosphate.

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-1,2-mannosyltransferase alg9 (EC:2.4.1.259, EC:2.4.1.261)
Alternative name(s):
Asparagine-linked glycosylation protein 9
Dol-P-Man:Man(6)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Dol-P-Man:Man(8)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase
Gene namesi
Name:alg9
ORF Names:SPAC1834.05
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1834.05.
PomBaseiSPAC1834.05. alg9.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 6839ExtracellularSequence analysisAdd
BLAST
Transmembranei69 – 8921HelicalSequence analysisAdd
BLAST
Topological domaini90 – 956CytoplasmicSequence analysis
Transmembranei96 – 11621HelicalSequence analysisAdd
BLAST
Topological domaini117 – 13620ExtracellularSequence analysisAdd
BLAST
Transmembranei137 – 15923HelicalSequence analysisAdd
BLAST
Topological domaini160 – 17617CytoplasmicSequence analysisAdd
BLAST
Transmembranei177 – 19721HelicalSequence analysisAdd
BLAST
Topological domaini198 – 21720ExtracellularSequence analysisAdd
BLAST
Transmembranei218 – 23821HelicalSequence analysisAdd
BLAST
Topological domaini239 – 28042CytoplasmicSequence analysisAdd
BLAST
Transmembranei281 – 30121HelicalSequence analysisAdd
BLAST
Topological domaini302 – 3054ExtracellularSequence analysis
Transmembranei306 – 32621HelicalSequence analysisAdd
BLAST
Topological domaini327 – 3337CytoplasmicSequence analysis
Transmembranei334 – 35421HelicalSequence analysisAdd
BLAST
Topological domaini355 – 37521ExtracellularSequence analysisAdd
BLAST
Transmembranei376 – 39621HelicalSequence analysisAdd
BLAST
Topological domaini397 – 577181CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929Sequence analysisAdd
BLAST
Chaini30 – 577548Alpha-1,2-mannosyltransferase alg9PRO_0000012142Add
BLAST

Proteomic databases

MaxQBiQ9P7Q9.

Interactioni

Protein-protein interaction databases

BioGridi278797. 40 interactions.
IntActiQ9P7Q9. 2 interactions.
MINTiMINT-4706119.

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 22 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000205434.
InParanoidiQ9P7Q9.
KOiK03846.
OMAiSFYFRNG.
OrthoDBiEOG70PC6J.
PhylomeDBiQ9P7Q9.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9P7Q9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSKAPRKSL SVSFVWTFSI LAVLRLTSAS FRVIDDCDEV YNYWEPLHYL
60 70 80 90 100
LYGYGLQTWE YSPEYAIRSW FYIALHAVPG FLARGLGLSR LHVFYFIRGV
110 120 130 140 150
LACFSAFCET NLILAVARNF NRAVALHLTS VLFVNSGMWS ASTSFLPSSF
160 170 180 190 200
AMNMVTLALS AQLSPPSTKR TVKVVSFITI GAVIGWPFSA ALSIPFILLE
210 220 230 240 250
LVDLKGRFRH LFCRWFKAIF VALLITGICI TVDSLFYHRI QFVAWNIVKY
260 270 280 290 300
NVLAKDGRGP DIYGTEPWWY YFANLSLQHN IVLWFAMACG PLVLLAAFTN
310 320 330 340 350
WINLDSFLDL SSVISPFYIW LFIFIIQPHK EERFMYPIYP VLCLAAAIGL
360 370 380 390 400
DMSLKLMIQI LSSINETVRS KFPVRFVVLC VYAIIGCLSI ARILAIQNYN
410 420 430 440 450
APMIIYPAIS FLETDNNVTT NVCVGKEWYR YPSTFFLPDN SRLKFVKSEF
460 470 480 490 500
DGILPGEFVE SNSTWWNREG YYQIPEHMNE FNNEEPTRYT SLESCDFLID
510 520 530 540 550
LEFDHSKATV NEPIYSKSDG WIPVMVYPFI DTKQTPFMGR AFAVPFIEPK
560 570
WGRYEILVKK PVKIDFSNLR RASKQQA
Length:577
Mass (Da):66,270
Last modified:October 1, 2000 - v1
Checksum:i0CD4F397A5C0546A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB75773.1.
PIRiT50116.
RefSeqiNP_594684.1. NM_001020113.2.

Genome annotation databases

EnsemblFungiiSPAC1834.05.1; SPAC1834.05.1:pep; SPAC1834.05.
GeneIDi2542331.
KEGGispo:SPAC1834.05.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB75773.1.
PIRiT50116.
RefSeqiNP_594684.1. NM_001020113.2.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278797. 40 interactions.
IntActiQ9P7Q9. 2 interactions.
MINTiMINT-4706119.

Protein family/group databases

CAZyiGT22. Glycosyltransferase Family 22.

Proteomic databases

MaxQBiQ9P7Q9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1834.05.1; SPAC1834.05.1:pep; SPAC1834.05.
GeneIDi2542331.
KEGGispo:SPAC1834.05.

Organism-specific databases

EuPathDBiFungiDB:SPAC1834.05.
PomBaseiSPAC1834.05. alg9.

Phylogenomic databases

HOGENOMiHOG000205434.
InParanoidiQ9P7Q9.
KOiK03846.
OMAiSFYFRNG.
OrthoDBiEOG70PC6J.
PhylomeDBiQ9P7Q9.

Enzyme and pathway databases

UniPathwayiUPA00378.
ReactomeiR-SPO-446193. Biosynthesis of the N-glycan precursor (dolichol lipid-linked oligosaccharide, LLO) and transfer to a nascent protein.

Miscellaneous databases

PROiQ9P7Q9.

Family and domain databases

InterProiIPR005599. GPI_mannosylTrfase.
[Graphical view]
PANTHERiPTHR22760. PTHR22760. 1 hit.
PfamiPF03901. Glyco_transf_22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.

Entry informationi

Entry nameiALG9_SCHPO
AccessioniPrimary (citable) accession number: Q9P7Q9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: October 1, 2000
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.