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Protein

Probable L-lactate dehydrogenase

Gene

SPAC186.08c

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. Probable L-lactate dehydrogenase (SPAC186.08c)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051SubstrateBy similarity
Binding sitei137 – 1371NAD or substrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Active sitei192 – 1921Proton acceptorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiR-SPO-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable L-lactate dehydrogenase (EC:1.1.1.27)
Short name:
L-LDH
Gene namesi
ORF Names:SPAC186.08c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC186.08c.
PomBaseiSPAC186.08c.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Probable L-lactate dehydrogenasePRO_0000168500Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

BioGridi279025. 10 interactions.
MINTiMINT-4705999.

Structurei

3D structure databases

ProteinModelPortaliQ9P7P7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOGENOMiHOG000213793.
InParanoidiQ9P7P7.
KOiK00016.
OMAiVCSKDYF.
OrthoDBiEOG092C3G32.
PhylomeDBiQ9P7P7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P7P7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAGSKVFSN DSVRSSSFKS IKIVIVGAGN VGSTTAFTLL LSGLAAEIVI
60 70 80 90 100
IDLNKKKAEG EAMDLNHAAP LSHETRVYLG DYKDCKDATA VVITAGKNQK
110 120 130 140 150
PGETRMDLLK ANISIFKEIL REVTKYTKDA ILLVATNPVD VLTYATLKLT
160 170 180 190 200
GFPAERVIGS GTIIDTARFQ YLIGKLYGLD PQSVNADIIG EHGDSELAVW
210 220 230 240 250
SHASIAGLSL ADFCEESETK YDEQALNECF KETKNAAYDI IQRKGSTEYG
260 270 280 290 300
VAAGLVRILA AIIRDENALL TVSGLDSYSN IGDVCFSMPR KLNKDGAHRI
310 320 330
INAKLSKDED AKLVESVKSI KHAIESIGLN
Length:330
Mass (Da):35,592
Last modified:October 1, 2000 - v1
Checksum:i5F68D53F3EA7CDC0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB75872.1.
PIRiT50135.
RefSeqiNP_595026.1. NM_001020456.1.

Genome annotation databases

EnsemblFungiiSPAC186.08c.1; SPAC186.08c.1:pep; SPAC186.08c.
GeneIDi2542569.
KEGGispo:SPAC186.08c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB75872.1.
PIRiT50135.
RefSeqiNP_595026.1. NM_001020456.1.

3D structure databases

ProteinModelPortaliQ9P7P7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279025. 10 interactions.
MINTiMINT-4705999.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC186.08c.1; SPAC186.08c.1:pep; SPAC186.08c.
GeneIDi2542569.
KEGGispo:SPAC186.08c.

Organism-specific databases

EuPathDBiFungiDB:SPAC186.08c.
PomBaseiSPAC186.08c.

Phylogenomic databases

HOGENOMiHOG000213793.
InParanoidiQ9P7P7.
KOiK00016.
OMAiVCSKDYF.
OrthoDBiEOG092C3G32.
PhylomeDBiQ9P7P7.

Enzyme and pathway databases

UniPathwayiUPA00554; UER00611.
ReactomeiR-SPO-70268. Pyruvate metabolism.

Miscellaneous databases

PROiQ9P7P7.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.110.10. 1 hit.
HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiIPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11540. PTHR11540. 1 hit.
PfamiPF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiPS00064. L_LDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLDH_SCHPO
AccessioniPrimary (citable) accession number: Q9P7P7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.