Q9P7K5 (GIT1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Adenylate cyclase activation protein git1

Alternative name(s):
Glucose-insensitive transcription protein 1

Gene names

Name:	git1
ORF Names:	SPBC21C3.20c

Organism

Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)

Taxonomic identifier

284812 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	1098 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the glucose/cAMP-signaling pathway. Required for maintaining basal cAMP levels and for producing a glucose-triggered cAMP response. Ref.2
Subunit structure	Interacts with cyr1. Ref.2
Subcellular location	Cytoplasm. Nucleus. Cytoplasm › cytoskeleton › spindle pole body. Note: Septum. Ref.2 Ref.3
Sequence similarities	Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Nucleus
Molecular function	Transducer
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cAMP-mediated signaling Non-traceable author statement. Source: GeneDB_Spombe conjugation with cellular fusion Non-traceable author statement. Source: GeneDB_Spombe meiosis Inferred from mutant phenotype. Source: GeneDB_Spombe
Cellular component	cell division site Inferred from direct assay. Source: GeneDB_Spombe cytosol Inferred from direct assay. Source: GeneDB_Spombe nucleus Inferred from direct assay. Source: GeneDB_Spombe spindle pole body Inferred from direct assay. Source: GeneDB_Spombe
Molecular function	protein binding Inferred from physical interaction Ref.2. Source: IntAct signal transducer activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
cyr1	P14605	3	EBI-1542810,EBI-1542835

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1098	1098	Adenylate cyclase activation protein git1		PRO_0000256253
Regions
	Domain	567 – 700	134	MHD1
	Domain	981 – 1097	117	MHD2

Sequences

	Sequence		Length	Mass (Da)
	Q9P7K5 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8C3697EF3E16EFF2	FASTA	1,098	128,300
	10 20 30 40 50 60 MGFTSVEPPD FFDWTARAIF LLSTLRSRRN AKQIERENAL LDQIKELLSI AIIQCHERNI 70 80 90 100 110 120 PSIDLPPSFF NYVCEQLPRH PFAKIASSYI DTHTLFFQIS ISSFLCRFSN CSEDEIRLFK 130 140 150 160 170 180 QHQSLDSFLL SLFDLLCSHH FSVEFTSISL IIKSSQWLFY TLSDYAHESL TGVEADITTK 190 200 210 220 230 240 FLSTLADTWP EFEEQCTLSK HLFLLGDFSL ISYVKVLCGV FNIPNYNAFI LSVEDLLPEN 250 260 270 280 290 300 ISFAAVADFQ LSKYRAINDN SVLRSDDFSS KAVFDKYVER ECYYSENAIS KIFQNYPDLA 310 320 330 340 350 360 VLFEELQYAE GNNDLLSAIL NTNKYTLRPD DCAPYFYRLM DLCLRYCYSQ SNEIGTNVLW 370 380 390 400 410 420 SMTTSFINIW RIHPGTCCCV VLSIYWPYLE DKLLKFSDLG DIFEYFFSLI HDCDYAIWSV 430 440 450 460 470 480 HDEHQFSELL AFYHRRALND LVEELGSCPR SSDSCRAILD ILNNYIFSCP LFLVTPEELE 490 500 510 520 530 540 EQYTDFFKKL KEKYGEFFQN LLKKELTGKP ESDLEGLRLV GVQLQATYEN LKSNFSARIF 550 560 570 580 590 600 NQLSVHSFFA EATCHLFLEK LPTITKTYVY SNCKEIPDHI LEMINALYQQ TRALVACTGY 610 620 630 640 650 660 LSSDYPKIYE AFSGTFVYRN LSLIIDKGLA NVLQTVLRES FIPKRSRFYS DSTKMILAPL 670 680 690 700 710 720 QDCYDFLQKI DWPSSKDANR FYLHLGKLSE NYIHYYVDSM YALFIYSTDI PLKAEPLVPD 730 740 750 760 770 780 LPVKQLKEKA SHFKRHSGDP NFLKLYRESF TLINTVSLVS GWLKMLHQRI DYKILTEKVM 790 800 810 820 830 840 HFKDMEVPSA TVIISIKMAH LLVTPKAPYS FSVLLQDPES EFCVHTRDVY DELVPNWKET 850 860 870 880 890 900 YQMEITNVKR FKLIIYQRNI HTGLETVYGH ATLEVDPDKL KQGHGKDVWL DITNKGQILI 910 920 930 940 950 960 GLRVLLKRET PNYYCFAQME YLTVTEVKML NVLMISLSKF IRNSLRNSGL MDFVRYLDGD 970 980 990 1000 1010 1020 ENDNAEAETK EKMDELQARC EKEQQSLLAE LDDFFYWIYV NLLPKPLFYL LSRLWFGLLE 1030 1040 1050 1060 1070 1080 TFRDILVPPV TDSTLRRIPL NKRELKMVYS WLQLFYDFFR NFQDIAPFDF LNTDEYKSVM 1090 AIKDYYFVKS KDVKRVSS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 972 / ATCC 24843.
[2]	"Schizosaccharomyces pombe Git1 is a C2-domain protein required for glucose activation of adenylate cyclase." Kao R.S., Morreale E., Wang L., Ivey F.D., Hoffman C.S. Genetics 173:49-61(2006) [PubMed: 16489217] [Abstract] Cited for: FUNCTION, INTERACTION WITH CYR1, SUBCELLULAR LOCATION.
[3]	"ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe." Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M. Nat. Biotechnol. 24:841-847(2006) [PubMed: 16823372] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CU329671 Genomic DNA. Translation: CAB76056.1.
PIR	T50364.
RefSeq	NP_596600.1. NM_001022520.1.
3D structure databases
ProteinModelPortal	Q9P7K5.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9P7K5. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	SPBC21C3.20c.1; SPBC21C3.20c.1:pep; SPBC21C3.20c.
GeneID	2540617.
GenomeReviews	Gene locus git1 in contig CU329671_GR.
KEGG	spo:SPBC21C3.20c.
NMPDR	fig|4896.1.peg.2466.
Organism-specific databases
GeneDB_Spombe	SPBC21C3.20c.
Phylogenomic databases
eggNOG	fuNOG04153.
GeneTree	EFGT00050000003282.
OrthoDB	EOG41K2P7.
Gene expression databases
ArrayExpress	Q9P7K5.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR014770. Munc13_1. IPR014772. Munc13_dom-2. [Graphical view]
SMART	SM00239. C2. 1 hit. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 1 hit.
PROSITE	PS51258. MHD1. 1 hit. PS51259. MHD2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GIT1_SCHPO

Accession

Primary (citable) accession number: Q9P7K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	October 1, 2000
Last modified:	November 16, 2011
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program