Reviewed, UniProtKB/Swiss-Prot Q9P7I2 (KCC1_SCHPO)

Names and origin

Protein names

Recommended name:
    Calcium/calmodulin-dependent protein kinase type I
      Short name=CaMK-I
    EC=2.7.11.17

Gene names

Name:	cmk1
ORF Names:	SPACUNK12.02c, SPAC25D11.02c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	335 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important in cell cycle regulation.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm Ref.1.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	calcium-mediated signaling Inferred by curator. Source: GeneDB_SPombe protein amino acid phosphorylation Ref.1 Traceable author statement. Source: GeneDB_SPombe regulation of mitotic cell cycle Ref.1 Inferred from mutant phenotype. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin binding Inferred from electronic annotation. Source: UniProtKB-KW calmodulin-dependent protein kinase activity Ref.1 Traceable author statement. Source: GeneDB_SPombe
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 335	335	Calcium/calmodulin-dependent protein kinase type I		PRO_0000086089
Regions
	Domain	31 – 291	261	Protein kinase
	Nucleotide binding	37 – 45	9	ATP By similarity
	Region	310 – 334	25	Calmodulin-binding Potential
Sites
	Active site	154	1	Proton acceptor By similarity
Amino acid modifications
	Modified residue	192	1	Phosphothreonine; by autocatalysis Ref.1
Experimental info
	Mutagenesis	192	1	T → D: 15-fold increase in activity. Ref.1
	Sequence conflict	115	1	E → A in AAC26005. Ref.1
	Sequence conflict	294 – 295	2	KR → NG in AAC26005. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9P7I2-1 [UniParc]. Last modified October 1, 2000. Version 1. Checksum: 8761BBCFF6882B02	FASTA	335	38,164
	10 20 30 40 50 60 MQQTYKPNTS ALKDGAPKAT VDQKQLLPCK YRVGRVLGGG TYATVREAVH IETNKMYAAK 70 80 90 100 110 120 IMNKKMMEKK QDFVKNEIAI LKRVSYEHPN ILHLVDFFET VNNLYLITEL ATGGELFDRI 130 140 150 160 170 180 CAKGSFYEAD AAALMRTTTS AVKYLHDNGI VHRDLKPENL LYRSKDPNSD LLIADFGLSH 190 200 210 220 230 240 FYEDSQYYML MTACGTPEYM APEVFRRTGY GKPVDMWAIG VITYFLLSGY TPFARPSQVE 250 260 270 280 290 300 VIEAILANEY TFNDPCWSGI SETAKDFIKK CLENDPSKRL TAADALKHPF LSEKRPATSN 310 320 330 LLPNVRENFN ARKTFRTAYN AVRAFNTWKK LENKH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning of a calmodulin kinase I homologue from Schizosaccharomyces pombe." Rasmussen C.D. J. Biol. Chem. 275:685-690(2000) [PubMed: 10617667] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, PHOSPHORYLATION AT THR-192, MUTAGENESIS OF THR-192.
[2]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF073893 mRNA. Translation: AAC26005.1. CU329670 Genomic DNA. Translation: CAB76233.1.
PIR	T50290.
RefSeq	NP_593464.1.
3D structure databases
SMR	Q9P7I2. Positions 27-324.
ModBase	Search...
Genome annotation databases
GeneID	2542442.
GenomeReviews	Gene locus cmk1 in contig CU329670_GR.
KEGG	spo:SPACUNK12.02c.
NMPDR	fig|4896.1.peg.3434.
Organism-specific databases
GeneDB_Spombe	SPACUNK12.02c.
Phylogenomic databases
eggNOG	fuNOG05859.
HOGENOM	HBG755340.
OMA	FDRICAK.
OrthoDB	EOG98WDJ8.
PhylomeDB	Q9P7I2.
Enzyme and pathway databases
BRENDA	2.7.11.17. 653.
Gene expression databases
ArrayExpress	Q9P7I2.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_prot_kinase-like. IPR015733. Ca/CaM-dep_prot_kinase4. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22982. Ca/CaM-dep_prot_kinase-like. 1 hit. PTHR22982:SF33. CaMKIV. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KCC1_SCHPO

Accession

Primary (citable) accession number: Q9P7I2
Secondary accession number(s): O74235

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 14, 2001
Last sequence update:	October 1, 2000
Last modified:	January 19, 2010
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)