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Q9P7H1

- FLP1_SCHPO

UniProt

Q9P7H1 - FLP1_SCHPO

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Protein

Tyrosine-protein phosphatase CDC14 homolog

Gene

clp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15.11 Publications

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei286 – 2861Phosphocysteine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. phosphoprotein phosphatase activity Source: PomBase
  2. protein serine/threonine phosphatase activity Source: PomBase
  3. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
  4. protein tyrosine phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. attachment of spindle microtubules to kinetochore Source: PomBase
  2. cellular protein localization Source: PomBase
  3. cellular response to DNA damage stimulus Source: PomBase
  4. cellular response to hydrogen peroxide Source: PomBase
  5. chromosome passenger complex localization to spindle midzone Source: PomBase
  6. cytokinesis checkpoint Source: PomBase
  7. meiotic nuclear division Source: UniProtKB-KW
  8. mitotic actomyosin contractile ring maintenance Source: PomBase
  9. mitotic barrier septum assembly Source: PomBase
  10. mitotic cytokinesis Source: PomBase
  11. mitotic sister chromatid segregation Source: PomBase
  12. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: PomBase
  13. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
  14. positive regulation of attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation Source: PomBase
  15. positive regulation of septation initiation signaling Source: PomBase
  16. protein dephosphorylation Source: PomBase
  17. regulation of exit from mitosis Source: PomBase
  18. regulation of G2/M transition of mitotic cell cycle Source: PomBase
  19. sister chromatid biorientation Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein phosphatase

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis, Septation

Enzyme and pathway databases

ReactomeiREACT_246026. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein phosphatase CDC14 homolog (EC:3.1.3.48)
Alternative name(s):
CDC fourteen-like phosphatase 1
Gene namesi
Name:clp1
Synonyms:flp1
ORF Names:SPAC1782.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC1782.09c.

Subcellular locationi

Nucleusnucleolus. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Cell septum
Note: Localizes to kinetochores in prometaphase. Cytoplasmic retention is mediated through the binding of rad24. Tethered to the contractile ring by mid1.

GO - Cellular componenti

  1. condensed nuclear chromosome kinetochore Source: PomBase
  2. cytoplasm Source: PomBase
  3. medial cortex Source: PomBase
  4. mitotic spindle pole body Source: PomBase
  5. nucleolus Source: PomBase
  6. nucleoplasm Source: PomBase
  7. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi286 – 2861C → A: Inactivates phosphatase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 537537Tyrosine-protein phosphatase CDC14 homologPRO_0000094874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei453 – 4531Phosphothreonine1 Publication
Modified residuei468 – 4681Phosphoserine1 Publication
Modified residuei470 – 4701Phosphoserine1 Publication
Modified residuei513 – 5131Phosphoserine1 Publication

Post-translational modificationi

Phosphorylated by cds1, chk1, pmk1, and cdc2 upon Hydroxylurea and H2O2 stress treatment. Phosphorylation regulates the nucleolar-to-nucleoplasmic transition. Is able to autodephosphorylate.3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P7H1.

Interactioni

Subunit structurei

Interacts with ark1 at the kinetochores. Interacts with bir1, cdc25, mid1, nbl1, pic1, and rad24.5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rad24P426563EBI-704737,EBI-704791

Protein-protein interaction databases

BioGridi278823. 239 interactions.
IntActiQ9P7H1. 4 interactions.
MINTiMINT-4705468.
STRINGi4896.SPAC1782.09c-1.

Structurei

3D structure databases

ProteinModelPortaliQ9P7H1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2453.
HOGENOMiHOG000198341.
InParanoidiQ9P7H1.
KOiK06639.
OMAiNDDETRT.
PhylomeDBiQ9P7H1.

Family and domain databases

Gene3Di3.90.190.10. 2 hits.
InterProiIPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view]
PANTHERiPTHR23339:SF27. PTHR23339:SF27. 1 hit.
PfamiPF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view]
SUPFAMiSSF52799. SSF52799. 2 hits.
PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9P7H1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYQDDGLGE MIEFLEDKLY YTSLSQPPKA ELYPHMHFFT IDDELIYNPF
60 70 80 90 100
YHDFGPLNVS HLIRFAVIVH GIMGKHRQAK KSKAIVLYSS TDTRLRANAA
110 120 130 140 150
CLLACYMVLV QNWPPHLALA PLAQAEPPFL GFRDAGYAVS DYYITIQDCV
160 170 180 190 200
YGLWRARESS ILNIRNIDVH DYETYERVEN GDFNWISPKF IAFASPIQAG
210 220 230 240 250
WNHASTRPKK LPQPFAIVLD YFVANKVKLI VRLNGPLYDK KTFENVGIRH
260 270 280 290 300
KEMYFEDGTV PELSLVKEFI DLTEEVEEDG VIAVHCKAGL GRTGCLIGAY
310 320 330 340 350
LIYKHCFTAN EVIAYMRIMR PGMVVGPQQH WLHINQVHFR AYFYEKAMGR
360 370 380 390 400
AIQQATAAEP LATPPRHPLN ATNGTSQSNI STPLPEPTPG QPRKVSGHNP
410 420 430 440 450
PSARRLPSAS SVKFNEKLKN ASKQSIQNEN KASYSSYEDS EIQNDDETRT
460 470 480 490 500
VGTPTETISV VRLRRSSSQS NIEPNGVRSP TSSPTGSPIR RTSGNRWSSG
510 520 530
SSHSKKSAQR SVSMSSLNNT SNGRVAKPKP SKSRLIS
Length:537
Mass (Da):60,253
Last modified:October 1, 2000 - v1
Checksum:iF5E50A8C0924C7EA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB76271.1.
PIRiT50099.
RefSeqiNP_594716.1. NM_001020143.2.

Genome annotation databases

EnsemblFungiiSPAC1782.09c.1; SPAC1782.09c.1:pep; SPAC1782.09c.
GeneIDi2542358.
KEGGispo:SPAC1782.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB76271.1 .
PIRi T50099.
RefSeqi NP_594716.1. NM_001020143.2.

3D structure databases

ProteinModelPortali Q9P7H1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 278823. 239 interactions.
IntActi Q9P7H1. 4 interactions.
MINTi MINT-4705468.
STRINGi 4896.SPAC1782.09c-1.

Proteomic databases

MaxQBi Q9P7H1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC1782.09c.1 ; SPAC1782.09c.1:pep ; SPAC1782.09c .
GeneIDi 2542358.
KEGGi spo:SPAC1782.09c.

Organism-specific databases

PomBasei SPAC1782.09c.

Phylogenomic databases

eggNOGi COG2453.
HOGENOMi HOG000198341.
InParanoidi Q9P7H1.
KOi K06639.
OMAi NDDETRT.
PhylomeDBi Q9P7H1.

Enzyme and pathway databases

Reactomei REACT_246026. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.

Miscellaneous databases

NextBioi 20803418.
PROi Q9P7H1.

Family and domain databases

Gene3Di 3.90.190.10. 2 hits.
InterProi IPR026070. CDC14.
IPR029260. DSPn.
IPR000340. Dual-sp_phosphatase_cat-dom.
IPR029021. Prot-tyrosine_phosphatase-like.
IPR000387. Tyr/Dual-sp_Pase.
IPR016130. Tyr_Pase_AS.
[Graphical view ]
PANTHERi PTHR23339:SF27. PTHR23339:SF27. 1 hit.
Pfami PF00782. DSPc. 1 hit.
PF14671. DSPn. 1 hit.
[Graphical view ]
SUPFAMi SSF52799. SSF52799. 2 hits.
PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
PS50056. TYR_PHOSPHATASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression."
    Trautmann S., Wolfe B.A., Jorgensen P., Tyers M., Gould K.L., McCollum D.
    Curr. Biol. 11:931-940(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  3. "Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis."
    Cueille N., Salimova E., Esteban V., Blanco M., Moreno S., Bueno A., Simanis V.
    J. Cell Sci. 114:2649-2664(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
  4. "The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase."
    Trautmann S., Rajagopalan S., McCollum D.
    Dev. Cell 7:755-762(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARK1.
  5. "A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast."
    Esteban V., Blanco M., Cueille N., Simanis V., Moreno S., Bueno A.
    J. Cell Sci. 117:2461-2468(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEPHOSPHORYLATION OF CDC25, INTERACTION WITH CDC25.
  6. "The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe."
    Mishra M., Karagiannis J., Trautmann S., Wang H., McCollum D., Balasubramanian M.K.
    J. Cell Sci. 117:3897-3910(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast."
    Mishra M., Karagiannis J., Sevugan M., Singh P., Balasubramanian M.K.
    Curr. Biol. 15:1376-1383(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RAD24, SUBCELLULAR LOCATION.
  8. "Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation."
    Trautmann S., McCollum D.
    Curr. Biol. 15:1384-1389(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-453; SER-468; SER-470 AND SER-513, IDENTIFICATION BY MASS SPECTROMETRY.
  10. "Cdc14-regulated midzone assembly controls anaphase B."
    Khmelinskii A., Lawrence C., Roostalu J., Schiebel E.
    J. Cell Biol. 177:981-993(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEPHOSPHORYLATION OF ASE1, MUTAGENESIS OF CYS-286.
  11. "The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1."
    Clifford D.M., Wolfe B.A., Roberts-Galbraith R.H., McDonald W.H., Yates J.R. III, Gould K.L.
    J. Cell Biol. 181:79-88(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH MID1, SUBCELLULAR LOCATION, FUNCTION IN DEPHOSPHORYLATION OF CDC15.
  12. "A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein."
    Bohnert K.A., Chen J.S., Clifford D.M., Vander Kooi C.W., Gould K.L.
    Mol. Biol. Cell 20:3646-3659(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH BIR1; NBL1 AND PIC1, FUNCTION, SUBCELLULAR LOCATION.
  13. "Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation."
    Chen J.S., Lu L.X., Ohi M.D., Creamer K.M., English C., Partridge J.F., Ohi R., Gould K.L.
    J. Cell Biol. 195:583-593(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN DEPHOSPHORYLATION OF NSK1.
  14. "A systematic screen reveals new elements acting at the G2/M cell cycle control."
    Navarro F.J., Nurse P.
    Genome Biol. 13:R36.1-R36.10(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  15. "Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress."
    Broadus M.R., Gould K.L.
    Mol. Biol. Cell 23:4118-4128(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiFLP1_SCHPO
AccessioniPrimary (citable) accession number: Q9P7H1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 6, 2002
Last sequence update: October 1, 2000
Last modified: November 26, 2014
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3