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Q9P7H1

- FLP1_SCHPO

UniProt

Q9P7H1 - FLP1_SCHPO

Protein

Tyrosine-protein phosphatase CDC14 homolog

Gene

clp1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Oct 2000)
      Previous versions | rss
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    Functioni

    Protein phosphatase which antagonizes mitotic cyclin-dependent kinase cdc2, the inactivation of which is essential for exit from mitosis. To access its substrates, is released from nucleolar sequestration during mitosis. Plays an essential in coordinating the nuclear division cycle with cytokinesis through the cytokinesis checkpoint. Involved in chromosome segregation, where it is required for meiosis I spindle dissambly as well as for establishing two consecutive chromosome segregation phases. Allows damaged actomyosin rings to be maintained to facilitate completion of cell division in response to minor perturbation of the cell division machinery. Dephosphorylates the mitotic inducer cdc25 for its rapid degradation. Down-regulation of cdc25 activity ensures a prompt inactivation of mitotic cdc2 complexes to trigger cell division. Dephosphorylates also cdc2-phosphorylated nsk1, allowing nsk1-binding to kinetochores and spindle. Dephosphorylates ase1, which is essential for spindle midzone assembly and for continuous extension of the anaphase spindle. Tethered to the contractile ring by mid1, where it dephosphorylates cdc15.11 Publications

    Catalytic activityi

    Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei286 – 2861Phosphocysteine intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. phosphoprotein phosphatase activity Source: PomBase
    2. protein binding Source: IntAct
    3. protein serine/threonine phosphatase activity Source: PomBase
    4. protein tyrosine/serine/threonine phosphatase activity Source: InterPro
    5. protein tyrosine phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. attachment of spindle microtubules to kinetochore Source: PomBase
    2. cellular protein localization Source: PomBase
    3. cellular response to DNA damage stimulus Source: PomBase
    4. cellular response to hydrogen peroxide Source: PomBase
    5. chromosome passenger complex localization to spindle midzone Source: PomBase
    6. cytokinesis checkpoint Source: PomBase
    7. meiotic nuclear division Source: UniProtKB-KW
    8. mitotic actomyosin contractile ring maintenance Source: PomBase
    9. mitotic barrier septum assembly Source: PomBase
    10. mitotic cytokinesis Source: PomBase
    11. mitotic sister chromatid segregation Source: PomBase
    12. negative regulation of cyclin-dependent protein serine/threonine kinase activity Source: PomBase
    13. negative regulation of G2/M transition of mitotic cell cycle Source: PomBase
    14. positive regulation of attachment of spindle microtubules to kinetochore involved in mitotic sister chromatid segregation Source: PomBase
    15. positive regulation of septation initiation signaling Source: PomBase
    16. protein dephosphorylation Source: PomBase
    17. regulation of exit from mitosis Source: PomBase
    18. regulation of G2/M transition of mitotic cell cycle Source: PomBase
    19. sister chromatid biorientation Source: PomBase

    Keywords - Molecular functioni

    Hydrolase, Protein phosphatase

    Keywords - Biological processi

    Cell cycle, Cell division, Meiosis, Mitosis, Septation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein phosphatase CDC14 homolog (EC:3.1.3.48)
    Alternative name(s):
    CDC fourteen-like phosphatase 1
    Gene namesi
    Name:clp1
    Synonyms:flp1
    ORF Names:SPAC1782.09c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC1782.09c.

    Subcellular locationi

    Nucleusnucleolus. Cytoplasmcytoskeletonmicrotubule organizing centerspindle pole body. Cell septum
    Note: Localizes to kinetochores in prometaphase. Cytoplasmic retention is mediated through the binding of rad24. Tethered to the contractile ring by mid1.

    GO - Cellular componenti

    1. cell septum Source: UniProtKB-SubCell
    2. condensed nuclear chromosome kinetochore Source: PomBase
    3. cytoplasm Source: PomBase
    4. medial cortex Source: PomBase
    5. mitotic spindle pole body Source: PomBase
    6. nucleolus Source: PomBase
    7. nucleoplasm Source: PomBase
    8. nucleus Source: PomBase

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Nucleus

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi286 – 2861C → A: Inactivates phosphatase activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 537537Tyrosine-protein phosphatase CDC14 homologPRO_0000094874Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei453 – 4531Phosphothreonine1 Publication
    Modified residuei468 – 4681Phosphoserine1 Publication
    Modified residuei470 – 4701Phosphoserine1 Publication
    Modified residuei513 – 5131Phosphoserine1 Publication

    Post-translational modificationi

    Phosphorylated by cds1, chk1, pmk1, and cdc2 upon Hydroxylurea and H2O2 stress treatment. Phosphorylation regulates the nucleolar-to-nucleoplasmic transition. Is able to autodephosphorylate.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9P7H1.

    Interactioni

    Subunit structurei

    Interacts with ark1 at the kinetochores. Interacts with bir1, cdc25, mid1, nbl1, pic1, and rad24.5 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rad24P426563EBI-704737,EBI-704791

    Protein-protein interaction databases

    BioGridi278823. 237 interactions.
    IntActiQ9P7H1. 4 interactions.
    MINTiMINT-4705468.
    STRINGi4896.SPAC1782.09c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9P7H1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG2453.
    HOGENOMiHOG000198341.
    KOiK06639.
    OMAiNDDETRT.
    PhylomeDBiQ9P7H1.

    Family and domain databases

    Gene3Di3.90.190.10. 2 hits.
    InterProiIPR026070. CDC14.
    IPR029260. DSPn.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view]
    PANTHERiPTHR23339:SF27. PTHR23339:SF27. 1 hit.
    PfamiPF00782. DSPc. 1 hit.
    PF14671. DSPn. 1 hit.
    [Graphical view]
    SUPFAMiSSF52799. SSF52799. 2 hits.
    PROSITEiPS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9P7H1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYQDDGLGE MIEFLEDKLY YTSLSQPPKA ELYPHMHFFT IDDELIYNPF    50
    YHDFGPLNVS HLIRFAVIVH GIMGKHRQAK KSKAIVLYSS TDTRLRANAA 100
    CLLACYMVLV QNWPPHLALA PLAQAEPPFL GFRDAGYAVS DYYITIQDCV 150
    YGLWRARESS ILNIRNIDVH DYETYERVEN GDFNWISPKF IAFASPIQAG 200
    WNHASTRPKK LPQPFAIVLD YFVANKVKLI VRLNGPLYDK KTFENVGIRH 250
    KEMYFEDGTV PELSLVKEFI DLTEEVEEDG VIAVHCKAGL GRTGCLIGAY 300
    LIYKHCFTAN EVIAYMRIMR PGMVVGPQQH WLHINQVHFR AYFYEKAMGR 350
    AIQQATAAEP LATPPRHPLN ATNGTSQSNI STPLPEPTPG QPRKVSGHNP 400
    PSARRLPSAS SVKFNEKLKN ASKQSIQNEN KASYSSYEDS EIQNDDETRT 450
    VGTPTETISV VRLRRSSSQS NIEPNGVRSP TSSPTGSPIR RTSGNRWSSG 500
    SSHSKKSAQR SVSMSSLNNT SNGRVAKPKP SKSRLIS 537
    Length:537
    Mass (Da):60,253
    Last modified:October 1, 2000 - v1
    Checksum:iF5E50A8C0924C7EA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB76271.1.
    PIRiT50099.
    RefSeqiNP_594716.1. NM_001020143.2.

    Genome annotation databases

    EnsemblFungiiSPAC1782.09c.1; SPAC1782.09c.1:pep; SPAC1782.09c.
    GeneIDi2542358.
    KEGGispo:SPAC1782.09c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CU329670 Genomic DNA. Translation: CAB76271.1 .
    PIRi T50099.
    RefSeqi NP_594716.1. NM_001020143.2.

    3D structure databases

    ProteinModelPortali Q9P7H1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278823. 237 interactions.
    IntActi Q9P7H1. 4 interactions.
    MINTi MINT-4705468.
    STRINGi 4896.SPAC1782.09c-1.

    Proteomic databases

    MaxQBi Q9P7H1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC1782.09c.1 ; SPAC1782.09c.1:pep ; SPAC1782.09c .
    GeneIDi 2542358.
    KEGGi spo:SPAC1782.09c.

    Organism-specific databases

    PomBasei SPAC1782.09c.

    Phylogenomic databases

    eggNOGi COG2453.
    HOGENOMi HOG000198341.
    KOi K06639.
    OMAi NDDETRT.
    PhylomeDBi Q9P7H1.

    Miscellaneous databases

    NextBioi 20803418.
    PROi Q9P7H1.

    Family and domain databases

    Gene3Di 3.90.190.10. 2 hits.
    InterProi IPR026070. CDC14.
    IPR029260. DSPn.
    IPR000340. Dual-sp_phosphatase_cat-dom.
    IPR029021. Prot-tyrosine_phosphatase-like.
    IPR000387. Tyr/Dual-sp_Pase.
    IPR016130. Tyr_Pase_AS.
    [Graphical view ]
    PANTHERi PTHR23339:SF27. PTHR23339:SF27. 1 hit.
    Pfami PF00782. DSPc. 1 hit.
    PF14671. DSPn. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52799. SSF52799. 2 hits.
    PROSITEi PS00383. TYR_PHOSPHATASE_1. 1 hit.
    PS50056. TYR_PHOSPHATASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    2. "Fission yeast Clp1p phosphatase regulates G2/M transition and coordination of cytokinesis with cell cycle progression."
      Trautmann S., Wolfe B.A., Jorgensen P., Tyers M., Gould K.L., McCollum D.
      Curr. Biol. 11:931-940(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    3. "Flp1, a fission yeast orthologue of the s. cerevisiae CDC14 gene, is not required for cyclin degradation or rum1p stabilisation at the end of mitosis."
      Cueille N., Salimova E., Esteban V., Blanco M., Moreno S., Bueno A., Simanis V.
      J. Cell Sci. 114:2649-2664(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, PHOSPHORYLATION.
    4. "The S. pombe Cdc14-like phosphatase Clp1p regulates chromosome biorientation and interacts with Aurora kinase."
      Trautmann S., Rajagopalan S., McCollum D.
      Dev. Cell 7:755-762(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ARK1.
    5. "A role for the Cdc14-family phosphatase Flp1p at the end of the cell cycle in controlling the rapid degradation of the mitotic inducer Cdc25p in fission yeast."
      Esteban V., Blanco M., Cueille N., Simanis V., Moreno S., Bueno A.
      J. Cell Sci. 117:2461-2468(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEPHOSPHORYLATION OF CDC25, INTERACTION WITH CDC25.
    6. "The Clp1p/Flp1p phosphatase ensures completion of cytokinesis in response to minor perturbation of the cell division machinery in Schizosaccharomyces pombe."
      Mishra M., Karagiannis J., Trautmann S., Wang H., McCollum D., Balasubramanian M.K.
      J. Cell Sci. 117:3897-3910(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The 14-3-3 protein rad24p modulates function of the cdc14p family phosphatase clp1p/flp1p in fission yeast."
      Mishra M., Karagiannis J., Sevugan M., Singh P., Balasubramanian M.K.
      Curr. Biol. 15:1376-1383(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH RAD24, SUBCELLULAR LOCATION.
    8. "Distinct nuclear and cytoplasmic functions of the S. pombe Cdc14-like phosphatase Clp1p/Flp1p and a role for nuclear shuttling in its regulation."
      Trautmann S., McCollum D.
      Curr. Biol. 15:1384-1389(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-453; SER-468; SER-470 AND SER-513, IDENTIFICATION BY MASS SPECTROMETRY.
    10. "Cdc14-regulated midzone assembly controls anaphase B."
      Khmelinskii A., Lawrence C., Roostalu J., Schiebel E.
      J. Cell Biol. 177:981-993(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEPHOSPHORYLATION OF ASE1, MUTAGENESIS OF CYS-286.
    11. "The Clp1/Cdc14 phosphatase contributes to the robustness of cytokinesis by association with anillin-related Mid1."
      Clifford D.M., Wolfe B.A., Roberts-Galbraith R.H., McDonald W.H., Yates J.R. III, Gould K.L.
      J. Cell Biol. 181:79-88(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH MID1, SUBCELLULAR LOCATION, FUNCTION IN DEPHOSPHORYLATION OF CDC15.
    12. "A link between aurora kinase and Clp1/Cdc14 regulation uncovered by the identification of a fission yeast borealin-like protein."
      Bohnert K.A., Chen J.S., Clifford D.M., Vander Kooi C.W., Gould K.L.
      Mol. Biol. Cell 20:3646-3659(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH BIR1; NBL1 AND PIC1, FUNCTION, SUBCELLULAR LOCATION.
    13. "Cdk1 phosphorylation of the kinetochore protein Nsk1 prevents error-prone chromosome segregation."
      Chen J.S., Lu L.X., Ohi M.D., Creamer K.M., English C., Partridge J.F., Ohi R., Gould K.L.
      J. Cell Biol. 195:583-593(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN DEPHOSPHORYLATION OF NSK1.
    14. "A systematic screen reveals new elements acting at the G2/M cell cycle control."
      Navarro F.J., Nurse P.
      Genome Biol. 13:R36.1-R36.10(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    15. "Multiple protein kinases influence the redistribution of fission yeast Clp1/Cdc14 phosphatase upon genotoxic stress."
      Broadus M.R., Gould K.L.
      Mol. Biol. Cell 23:4118-4128(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION, SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiFLP1_SCHPO
    AccessioniPrimary (citable) accession number: Q9P7H1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 6, 2002
    Last sequence update: October 1, 2000
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3