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Protein

Adenylyl-sulfate kinase

Gene

met14

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of activated sulfate. Required for methionine synthesis.1 Publication

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (sua1)
  2. Adenylyl-sulfate kinase (met14)
  3. Probable phosphoadenosine phosphosulfate reductase (met16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei103Phosphoserine intermediateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi32 – 39ATPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-174362. Transport and synthesis of PAPS.
UniPathwayiUPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase
Short name:
APS kinase
Gene namesi
Name:met14
ORF Names:SPAC1782.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1782.11.
PomBaseiSPAC1782.11. met14.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to methionine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001059331 – 202Adenylyl-sulfate kinaseAdd BLAST202

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P7G9.
PRIDEiQ9P7G9.

Interactioni

Protein-protein interaction databases

BioGridi278824. 25 interactors.
MINTiMINT-4705440.
STRINGi4896.SPAC1782.11.1.

Structurei

3D structure databases

ProteinModelPortaliQ9P7G9.
SMRiQ9P7G9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000228204.
InParanoidiQ9P7G9.
KOiK00860.
OMAiVSPFRAD.
OrthoDBiEOG092C20R5.
PhylomeDBiQ9P7G9.

Family and domain databases

CDDicd02027. APSK. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiView protein in InterPro
IPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P7G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNITFHPG SVTKEERIKF VGHPGMTIWM TGLSASGKST IACALEQYLL
60 70 80 90 100
QRGVTTYRLD GDNVRFGLNS DLGFSEQDRN ENIRRIGHVA KLFADACVVA
110 120 130 140 150
VTSFISPYRK DRDQAREFHK KDGLPFIEVY VECPVEVAEQ RDPKGLYKRA
160 170 180 190 200
RAGEIKEFTG ISAPYEAPIS PEIVVSSHTQ SIEECVEKIV NYLLEKDLIT

LK
Length:202
Mass (Da):22,669
Last modified:October 1, 2000 - v1
Checksum:iA5F7E4D53D76353B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB76273.1.
PIRiT50101.
RefSeqiNP_594718.1. NM_001020145.2.

Genome annotation databases

EnsemblFungiiSPAC1782.11.1; SPAC1782.11.1:pep; SPAC1782.11.
GeneIDi2542359.
KEGGispo:SPAC1782.11.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiKAPS_SCHPO
AccessioniPrimary (citable) accession number: Q9P7G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 9, 2003
Last sequence update: October 1, 2000
Last modified: July 5, 2017
This is version 115 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families