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Protein

Adenylyl-sulfate kinase

Gene

met14

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the synthesis of activated sulfate. Required for methionine synthesis.1 Publication

Catalytic activityi

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate.

Pathwayi: hydrogen sulfide biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes sulfite from sulfate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Sulfate adenylyltransferase (sua1)
  2. Adenylyl-sulfate kinase (met14)
  3. Probable phosphoadenosine phosphosulfate reductase (met16)
This subpathway is part of the pathway hydrogen sulfide biosynthesis, which is itself part of Sulfur metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sulfite from sulfate, the pathway hydrogen sulfide biosynthesis and in Sulfur metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei103 – 1031Phosphoserine intermediateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi32 – 398ATPBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis, Methionine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-SPO-174362. Transport and synthesis of PAPS.
UniPathwayiUPA00140; UER00205.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylyl-sulfate kinase (EC:2.7.1.25)
Alternative name(s):
ATP adenosine-5'-phosphosulfate 3'-phosphotransferase
Adenosine-5'-phosphosulfate kinase
Short name:
APS kinase
Gene namesi
Name:met14
ORF Names:SPAC1782.11
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1782.11.
PomBaseiSPAC1782.11. met14.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Leads to methionine auxotrophy.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 202202Adenylyl-sulfate kinasePRO_0000105933Add
BLAST

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9P7G9.

Interactioni

Protein-protein interaction databases

BioGridi278824. 25 interactions.
MINTiMINT-4705440.

Structurei

3D structure databases

ProteinModelPortaliQ9P7G9.
SMRiQ9P7G9. Positions 2-202.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the APS kinase family.Curated

Phylogenomic databases

HOGENOMiHOG000228204.
InParanoidiQ9P7G9.
KOiK00860.
OMAiNIKWHNL.
OrthoDBiEOG092C20R5.
PhylomeDBiQ9P7G9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9P7G9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNITFHPG SVTKEERIKF VGHPGMTIWM TGLSASGKST IACALEQYLL
60 70 80 90 100
QRGVTTYRLD GDNVRFGLNS DLGFSEQDRN ENIRRIGHVA KLFADACVVA
110 120 130 140 150
VTSFISPYRK DRDQAREFHK KDGLPFIEVY VECPVEVAEQ RDPKGLYKRA
160 170 180 190 200
RAGEIKEFTG ISAPYEAPIS PEIVVSSHTQ SIEECVEKIV NYLLEKDLIT

LK
Length:202
Mass (Da):22,669
Last modified:October 1, 2000 - v1
Checksum:iA5F7E4D53D76353B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB76273.1.
PIRiT50101.
RefSeqiNP_594718.1. NM_001020145.2.

Genome annotation databases

EnsemblFungiiSPAC1782.11.1; SPAC1782.11.1:pep; SPAC1782.11.
GeneIDi2542359.
KEGGispo:SPAC1782.11.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB76273.1.
PIRiT50101.
RefSeqiNP_594718.1. NM_001020145.2.

3D structure databases

ProteinModelPortaliQ9P7G9.
SMRiQ9P7G9. Positions 2-202.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278824. 25 interactions.
MINTiMINT-4705440.

Proteomic databases

MaxQBiQ9P7G9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1782.11.1; SPAC1782.11.1:pep; SPAC1782.11.
GeneIDi2542359.
KEGGispo:SPAC1782.11.

Organism-specific databases

EuPathDBiFungiDB:SPAC1782.11.
PomBaseiSPAC1782.11. met14.

Phylogenomic databases

HOGENOMiHOG000228204.
InParanoidiQ9P7G9.
KOiK00860.
OMAiNIKWHNL.
OrthoDBiEOG092C20R5.
PhylomeDBiQ9P7G9.

Enzyme and pathway databases

UniPathwayiUPA00140; UER00205.
ReactomeiR-SPO-174362. Transport and synthesis of PAPS.

Miscellaneous databases

PROiQ9P7G9.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
HAMAPiMF_00065. Adenylyl_sulf_kinase. 1 hit.
InterProiIPR002891. APS_kinase.
IPR027417. P-loop_NTPase.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00455. apsK. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiKAPS_SCHPO
AccessioniPrimary (citable) accession number: Q9P7G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 9, 2003
Last sequence update: October 1, 2000
Last modified: September 7, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.